FastQCFastQC Report
Thu 2 Feb 2017
SRR4063943_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063943_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1311055
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT25280.19282181144193034No Hit
TATCAACGCAGAGTACTTTTTTTTT17210.13126832970394073No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGTGCG607.679955E-817.4171229
CGCGGTC755.8411388E-815.200397510
AACCGCG801.9998442E-613.0618457
GTCGGCG957.3769115E-813.00083612
GCGTTAT905.3985787E-712.667481
AAGACGG1850.012.3222945
AATTCCG853.9492443E-612.2920935
TGCGTAC550.003064773512.09260916
CCGTGCG550.003066526512.09168613
GTGCGCT550.003066526512.09168611
CGGTCCA1900.012.00031410
ATAACGA802.8636705E-511.87576412
TACCGGC802.8670283E-511.8744047
TTATACT1052.7269198E-711.759074
CTTATAC907.4811614E-611.607873
TCGCGTA752.072838E-411.4002989
TCTATAC1104.983249E-711.2241393
ATTCCGA855.3238702E-511.1759096
GCGGTCG1201.2738565E-711.0836239
CGGTCGG1201.2738565E-711.08362310
Produced by FastQC (version 0.11.5)