##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063943_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1311055
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.234071034395964	32.0	32.0	32.0	32.0	32.0
2	30.833452448600553	32.0	32.0	32.0	32.0	32.0
3	30.836671993165808	32.0	32.0	32.0	32.0	32.0
4	30.874964055665092	32.0	32.0	32.0	32.0	32.0
5	30.732807548119645	32.0	32.0	32.0	32.0	32.0
6	34.396761386822064	36.0	36.0	36.0	32.0	36.0
7	34.356213888814736	36.0	36.0	36.0	32.0	36.0
8	34.30676439966287	36.0	36.0	36.0	32.0	36.0
9	34.47007028690635	36.0	36.0	36.0	32.0	36.0
10	34.11747028156714	36.0	36.0	36.0	32.0	36.0
11	34.43298717445111	36.0	36.0	36.0	32.0	36.0
12	34.23355847008707	36.0	36.0	36.0	32.0	36.0
13	34.33276559717174	36.0	36.0	36.0	32.0	36.0
14	34.231878143937514	36.0	36.0	36.0	32.0	36.0
15	34.1439939590635	36.0	36.0	36.0	32.0	36.0
16	34.14396039830518	36.0	36.0	36.0	32.0	36.0
17	34.06835792548749	36.0	36.0	36.0	32.0	36.0
18	34.06899024068403	36.0	36.0	36.0	32.0	36.0
19	34.08497050085618	36.0	36.0	36.0	32.0	36.0
20	34.06114465068208	36.0	36.0	36.0	32.0	36.0
21	34.05394052881077	36.0	36.0	36.0	32.0	36.0
22	34.02713921231375	36.0	36.0	36.0	32.0	36.0
23	33.97061374236779	36.0	36.0	36.0	32.0	36.0
24	33.95630541815561	36.0	36.0	36.0	32.0	36.0
25	33.541499784524675	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	4.0
4	20.0
5	20.0
6	54.0
7	20.0
8	70.0
9	84.0
10	110.0
11	34.0
12	85.0
13	66.0
14	114.0
15	221.0
16	366.0
17	495.0
18	682.0
19	905.0
20	1331.0
21	1876.0
22	3071.0
23	4519.0
24	6844.0
25	10190.0
26	14479.0
27	19353.0
28	26891.0
29	36773.0
30	49286.0
31	67415.0
32	94754.0
33	133871.0
34	275919.0
35	561133.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.389480527308926	17.093208421621284	11.101963330711083	26.41534772035871
2	16.70141137505902	19.66291149214602	37.428327120476034	26.207350012318926
3	18.932867133933932	23.7333022126928	28.469360280146788	28.864470373226485
4	12.558467354392262	15.333473685495214	35.97187769834659	36.136181261765934
5	14.545586305800507	36.56363469929515	33.90984957129344	14.9809294236109
6	34.20118135554561	35.42998641357208	16.90758864171575	13.46124358916656
7	30.254221641721585	30.315942219163745	20.958675441313574	18.4711606978011
8	28.067506195749043	32.96298726666748	19.50493523458388	19.464571302999598
9	27.398781292806422	14.090605576138673	18.45490516290765	40.05570796814725
10	15.610203950802706	26.689821386596822	32.14575720665855	25.554217455941924
11	37.08543012697526	20.90360167712965	22.64560028206549	19.365367913829605
12	24.664221309410962	23.472071486249064	29.05052364532245	22.81318355901752
13	29.56705046930978	19.451207505293	25.27449546718037	25.707246558216852
14	23.495410918388764	19.335355963897953	25.4794970137577	31.68973610395558
15	25.143254010654374	27.111794605650786	22.24398211042083	25.500969273274006
16	25.772270539881468	25.551463713416705	23.737535252386095	24.93873049431573
17	24.060535428215115	25.704660440331207	25.27328941774834	24.96151471370534
18	24.74978245271209	24.96794039967635	26.075294261331543	24.206982886280016
19	25.465005511063342	24.937485306375432	25.411345157439325	24.1861640251219
20	25.685023526214707	24.365738747568756	25.079998290112883	24.869239436103655
21	26.391747068881287	24.231497313141183	24.630556912554958	24.74619870542257
22	25.660672901647903	24.343296479159225	24.927253463284753	25.068777155908116
23	24.468924811295405	24.29953342941593	25.34481337176637	25.88672838752229
24	24.765885011144697	24.845577234282924	25.25602271686361	25.132515037708774
25	24.8920843539679	24.509885493007523	25.21573587593155	25.38229427709303
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	165.0
1	165.0
2	325.0
3	485.0
4	485.0
5	485.0
6	1224.5
7	1964.0
8	1964.0
9	1964.0
10	2077.5
11	2191.0
12	2191.0
13	2191.0
14	2747.5
15	3304.0
16	3304.0
17	3304.0
18	5604.5
19	7905.0
20	7905.0
21	7905.0
22	12936.0
23	17967.0
24	17967.0
25	17967.0
26	26621.0
27	35275.0
28	35275.0
29	35275.0
30	44872.0
31	54469.0
32	54469.0
33	54469.0
34	68255.5
35	82042.0
36	82042.0
37	82042.0
38	95157.5
39	108273.0
40	108273.0
41	108273.0
42	123965.0
43	139657.0
44	139657.0
45	139657.0
46	155453.5
47	171250.0
48	171250.0
49	171250.0
50	175959.0
51	180668.0
52	180668.0
53	180668.0
54	169243.5
55	157819.0
56	157819.0
57	157819.0
58	143669.0
59	129519.0
60	129519.0
61	129519.0
62	112503.0
63	95487.0
64	95487.0
65	95487.0
66	77651.0
67	59815.0
68	59815.0
69	59815.0
70	45291.5
71	30768.0
72	30768.0
73	30768.0
74	23259.0
75	15750.0
76	15750.0
77	15750.0
78	12471.5
79	9193.0
80	9193.0
81	9193.0
82	6286.0
83	3379.0
84	3379.0
85	3379.0
86	2601.0
87	1823.0
88	1823.0
89	1823.0
90	1336.0
91	849.0
92	849.0
93	849.0
94	589.5
95	330.0
96	330.0
97	330.0
98	519.0
99	708.0
100	708.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03630663854681916
2	0.0048815648466311486
3	0.0021356846204011273
4	0.005110388198816983
5	0.010297050848362578
6	0.014339596736978998
7	0.023568805275141012
8	0.036535461899005
9	0.0495783929735976
10	0.05636681908844404
11	0.05499387897532902
12	0.062316226245275746
13	0.06353661745693354
14	0.06712151664117828
15	0.06063818832924629
16	0.06559602762660605
17	0.062087402893089914
18	0.07589307847496864
19	0.07345229605165306
20	0.0783338608982842
21	0.0743675894603964
22	0.0783338608982842
23	0.08168993673034312
24	0.07741856748954087
25	0.07657954853152614
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1311055.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.903734382841
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.36414499427012	45.954803712694975
2	12.361670538719991	14.315737748044668
3	3.6238529891714992	6.295038627823526
4	1.4960264376654147	3.4650206990514403
5	0.8023458983113423	2.3229411889490956
6	0.49686639515816083	1.7262251861398716
7	0.3462479309759708	1.4034333758088615
8	0.23466423114936072	1.087034824770095
9	0.1926951166801248	1.004199016780485
>10	0.9484157400429972	9.674059284164976
>50	0.06533633084265525	2.6639138344185085
>100	0.0615278221307277	7.228886909273115
>500	0.005545407341017046	2.2788146550903523
>1k	6.601675405972674E-4	0.5798909369899795
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2528	0.19282181144193034	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1721	0.13126832970394073	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.2882335218583506E-4
2	0.0	0.0	0.0	0.0	2.2882335218583506E-4
3	0.0	0.0	0.0	0.0	2.2882335218583506E-4
4	0.0	0.0	0.0	0.0	2.2882335218583506E-4
5	0.0	0.0	0.0	0.0	2.2882335218583506E-4
6	0.0	0.0	0.0	0.0	2.2882335218583506E-4
7	0.0	0.0	0.0	0.0	2.2882335218583506E-4
8	0.0	0.0	0.0	0.0	2.2882335218583506E-4
9	0.0	0.0	0.0	0.0	3.050978029144468E-4
10	0.0	0.0	0.0	7.62744507286117E-5	3.050978029144468E-4
11	0.0	0.0	0.0	7.62744507286117E-5	3.050978029144468E-4
12	0.0	0.0	0.0	7.62744507286117E-5	5.339211551002818E-4
13	0.0	0.0	0.0	7.62744507286117E-5	6.101956058288936E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAGTGCG	60	7.679955E-8	17.417122	9
CGCGGTC	75	5.8411388E-8	15.2003975	10
AACCGCG	80	1.9998442E-6	13.061845	7
GTCGGCG	95	7.3769115E-8	13.000836	12
GCGTTAT	90	5.3985787E-7	12.66748	1
AAGACGG	185	0.0	12.322294	5
AATTCCG	85	3.9492443E-6	12.292093	5
TGCGTAC	55	0.0030647735	12.092609	16
CCGTGCG	55	0.0030665265	12.091686	13
GTGCGCT	55	0.0030665265	12.091686	11
CGGTCCA	190	0.0	12.000314	10
ATAACGA	80	2.8636705E-5	11.875764	12
TACCGGC	80	2.8670283E-5	11.874404	7
TTATACT	105	2.7269198E-7	11.75907	4
CTTATAC	90	7.4811614E-6	11.60787	3
TCGCGTA	75	2.072838E-4	11.400298	9
TCTATAC	110	4.983249E-7	11.224139	3
ATTCCGA	85	5.3238702E-5	11.175909	6
GCGGTCG	120	1.2738565E-7	11.083623	9
CGGTCGG	120	1.2738565E-7	11.083623	10
>>END_MODULE