FastQCFastQC Report
Thu 2 Feb 2017
SRR4063943_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063943_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1311055
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGATC407.0299936E-427.50164427
TAACGGC1105.2514224E-922.00131636
GTATCAA19050.021.483241
ACAATCG600.007411518.3337323
TTAACGG1355.5228156E-817.92699835
TAGGACG2100.017.8078734
CGGTCCA3000.017.60038210
CTAGCGG2900.017.44865429
GTATAAA2450.017.0635471
GGTATCA9550.017.0494381
CGCAATA2850.016.98347336
GTCTATA1307.307535E-716.925381
TAGCGGC2950.016.40713530
CGGGTAA1351.086235E-616.29540644
GTATAGA1501.8350693E-716.1355291
AATACGA3150.016.06322939
CGACGGT2900.015.9295577
AACGCAG25050.015.8947336
ACGGTAT3050.015.8679869
CGTCTTA1259.436048E-615.84094715