##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063943_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1311055
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.232129849624922	32.0	32.0	32.0	32.0	32.0
2	31.27389163688785	32.0	32.0	32.0	32.0	32.0
3	31.393011734824245	32.0	32.0	32.0	32.0	32.0
4	31.503968178299157	32.0	32.0	32.0	32.0	32.0
5	31.41735167479625	32.0	32.0	32.0	32.0	32.0
6	35.024526812376294	36.0	36.0	36.0	36.0	36.0
7	35.04583484293184	36.0	36.0	36.0	36.0	36.0
8	34.97956149818276	36.0	36.0	36.0	36.0	36.0
9	35.12392920205483	36.0	36.0	36.0	36.0	36.0
10	34.93769063845529	36.0	36.0	36.0	36.0	36.0
11	35.12858499452731	36.0	36.0	36.0	36.0	36.0
12	35.015823134803654	36.0	36.0	36.0	36.0	36.0
13	35.06995663797476	36.0	36.0	36.0	36.0	36.0
14	35.01718387100465	36.0	36.0	36.0	36.0	36.0
15	34.97767751924977	36.0	36.0	36.0	36.0	36.0
16	34.99553718188787	36.0	36.0	36.0	36.0	36.0
17	34.95972403903726	36.0	36.0	36.0	36.0	36.0
18	34.95719782922913	36.0	36.0	36.0	36.0	36.0
19	34.9615706434894	36.0	36.0	36.0	36.0	36.0
20	34.95078848713441	36.0	36.0	36.0	36.0	36.0
21	34.94578488316661	36.0	36.0	36.0	36.0	36.0
22	34.934705256453775	36.0	36.0	36.0	36.0	36.0
23	34.878706842962345	36.0	36.0	36.0	32.0	36.0
24	34.84938465586875	36.0	36.0	36.0	32.0	36.0
25	34.82274961767432	36.0	36.0	36.0	32.0	36.0
26	34.75746402706218	36.0	36.0	36.0	32.0	36.0
27	34.756445763144946	36.0	36.0	36.0	32.0	36.0
28	34.72815938309224	36.0	36.0	36.0	32.0	36.0
29	34.707945890904654	36.0	36.0	36.0	32.0	36.0
30	34.68570273558318	36.0	36.0	36.0	32.0	36.0
31	34.688392172715865	36.0	36.0	36.0	32.0	36.0
32	34.63820663511447	36.0	36.0	36.0	32.0	36.0
33	34.61547761154185	36.0	36.0	36.0	32.0	36.0
34	34.60579151904383	36.0	36.0	36.0	32.0	36.0
35	34.56821491089237	36.0	36.0	36.0	32.0	36.0
36	34.540717208660205	36.0	36.0	36.0	32.0	36.0
37	34.53768987571078	36.0	36.0	36.0	32.0	36.0
38	34.50973757775227	36.0	36.0	36.0	32.0	36.0
39	34.49832386894524	36.0	36.0	36.0	32.0	36.0
40	34.47937729538425	36.0	36.0	36.0	32.0	36.0
41	34.459309487397555	36.0	36.0	36.0	32.0	36.0
42	34.39711224929542	36.0	36.0	36.0	32.0	36.0
43	34.392714264466406	36.0	36.0	36.0	32.0	36.0
44	34.33475636033576	36.0	36.0	36.0	32.0	36.0
45	34.29306093184496	36.0	36.0	36.0	32.0	36.0
46	34.27832928443124	36.0	36.0	36.0	32.0	36.0
47	34.25495574174997	36.0	36.0	36.0	32.0	36.0
48	34.21712742791111	36.0	36.0	36.0	32.0	36.0
49	34.19936463382543	36.0	36.0	36.0	32.0	36.0
50	33.57717563336397	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	0.0
19	4.0
20	12.0
21	37.0
22	131.0
23	366.0
24	1014.0
25	2378.0
26	4959.0
27	8865.0
28	14676.0
29	23219.0
30	33749.0
31	49076.0
32	72218.0
33	118634.0
34	246083.0
35	735632.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.452182529541936	18.247385640608634	11.739806687298284	26.560625142551146
2	16.113216297191254	20.493833940481203	36.83679010989682	26.55615965243073
3	18.1224347831393	24.349128064626115	28.854756763477184	28.6736803887574
4	12.361495131783181	16.08559518860765	36.10298576337377	35.4499239162354
5	14.278424627494651	36.9689296024957	33.5910392775284	15.161606492481248
6	34.35599808398141	35.83671738420749	16.5328301242056	13.274454407605496
7	30.276808031406816	30.5209629206447	20.86658586647527	18.335643181473213
8	28.014254180224462	33.26644058902227	19.235287028604468	19.484018202148807
9	27.496636268500524	13.903728631533344	18.181845918214798	40.41778918175133
10	15.491524622187235	26.946385026158765	32.16738251439895	25.394707837255048
11	37.36403125726991	20.802407221664996	22.59165328685677	19.24190823420833
12	24.518173192215105	23.626065175141793	29.0380549360361	22.817706696606997
13	29.69878272861309	19.40533024369073	25.17400215247814	25.72188487521805
14	23.451032797351132	19.395355329426543	25.384138374479708	31.769473498742613
15	25.238065453125287	27.29194254087006	22.158927950302978	25.31106405570167
16	25.82613802782524	25.64605198926043	23.662588479375152	24.865221503539175
17	24.15627109465278	25.79586668751502	25.2010785207333	24.8467836970989
18	24.876015407497807	25.023759582014414	25.99153350368026	24.10869150680752
19	25.661852477584844	24.942126760509666	25.280709047293975	24.115311714611515
20	25.81181300136456	24.31941799187977	24.99605277308899	24.87271623366668
21	26.57134902807281	24.290666676836594	24.533982174660864	24.60400212042973
22	25.70813362093952	24.319325244155078	24.959574448610734	25.012966686294668
23	24.540233874147912	24.21286193401152	25.409462818648	25.837441373192572
24	24.946912698848557	24.86377310365448	25.12936215912766	25.059952038369303
25	24.96922276615252	24.461608165768396	25.306437514206344	25.262731553872747
26	24.871565056541886	25.055855374816456	25.525810942237648	24.54676862640401
27	25.246115471749924	24.883940898723846	24.726347666231874	25.143595963294356
28	24.740042593959956	24.668874677341602	25.473842548803038	25.117240179895404
29	24.80183308212867	24.803282339803634	25.283062906936298	25.111821671131395
30	24.682990083905416	24.899923722349353	25.583829138062548	24.833257055682683
31	25.229051625959514	24.714462576347362	24.788988740217967	25.267497057475158
32	24.87362729147073	24.931216785926498	24.692620840684576	25.502535081918193
33	24.64986571288874	24.580071502833338	25.240179802089557	25.52988298218837
34	25.228448775606093	24.61579416269519	25.367043575364452	24.788713486334263
35	25.692481369325936	24.564572352613634	25.244429867049067	24.49851641101136
36	24.80971698213956	25.03779724808573	25.0003432646143	25.152142505160413
37	25.55648275136117	24.81575161593956	24.79157163757187	24.836193995127392
38	24.97559003463164	24.714937373182604	25.202907837124506	25.106564755061257
39	25.367602469879746	24.742275904942428	24.654098604495058	25.23602302068276
40	25.62004777929991	24.695582397935055	25.133177933652068	24.55119188911297
41	24.809308782474716	25.05346981739409	25.3816112645116	24.755610135619595
42	25.70888973470457	25.19105221928729	24.89349968612441	24.206558359883726
43	24.941058019046018	24.514753610696893	25.32007154652622	25.224116823730867
44	24.90465555484365	24.93875072843156	24.831431020963574	25.325162695761215
45	24.87748285522726	25.053068515782495	25.019049747490733	25.050398881499508
46	24.884404923717167	24.85839507811344	24.91224537429007	25.344954623879328
47	25.109779677888085	24.719019705804957	25.271331428985494	24.899869187321467
48	25.64685781408511	25.04565112926478	24.503634546882985	24.80385650976713
49	24.8467832296379	25.205960713961545	24.817417476513118	25.12983857988743
50	24.954501700179552	25.34762531673376	24.646577749844017	25.051295233242666
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	60.0
1	52.0
2	44.0
3	111.5
4	179.0
5	200.5
6	222.0
7	202.0
8	182.0
9	242.5
10	303.0
11	460.0
12	617.0
13	1135.0
14	1653.0
15	2125.0
16	2597.0
17	2755.5
18	2914.0
19	2995.5
20	3077.0
21	3566.0
22	4055.0
23	4530.0
24	5005.0
25	6242.5
26	7480.0
27	9205.0
28	10930.0
29	12931.0
30	14932.0
31	17339.0
32	19746.0
33	23010.5
34	26275.0
35	30826.5
36	35378.0
37	41802.5
38	48227.0
39	52956.0
40	57685.0
41	63554.5
42	69424.0
43	71672.5
44	73921.0
45	80767.5
46	87614.0
47	92404.5
48	97195.0
49	100421.5
50	103648.0
51	102210.0
52	100772.0
53	99211.0
54	97650.0
55	96747.0
56	95844.0
57	91907.5
58	87971.0
59	80564.0
60	73157.0
61	64181.0
62	55205.0
63	47653.5
64	40102.0
65	34152.5
66	28203.0
67	23848.0
68	19493.0
69	17223.0
70	14953.0
71	11727.5
72	8502.0
73	7327.0
74	6152.0
75	4803.5
76	3455.0
77	2987.0
78	2519.0
79	2061.5
80	1604.0
81	1272.0
82	940.0
83	770.0
84	600.0
85	437.0
86	274.0
87	191.5
88	109.0
89	90.0
90	71.0
91	59.0
92	47.0
93	38.0
94	29.0
95	24.0
96	19.0
97	16.0
98	13.0
99	10.5
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009305482988890626
2	5.339211551002818E-4
3	4.576467043716702E-4
4	0.0
5	0.0
6	2.288233521858351E-4
7	1.525489014572234E-4
8	7.62744507286117E-5
9	8.390189580147287E-4
10	0.0016780379160294574
11	0.0
12	2.288233521858351E-4
13	6.101956058288936E-4
14	3.050978029144468E-4
15	0.00541548600173143
16	0.0011441167609291755
17	0.0
18	3.813722536430585E-4
19	0.0
20	6.101956058288936E-4
21	0.0
22	2.288233521858351E-4
23	6.101956058288936E-4
24	5.339211551002818E-4
25	0.0016017634653008454
26	0.006101956058288936
27	0.006483328311931993
28	0.00541548600173143
29	0.002974703578415856
30	0.004195094790073643
31	0.00808509177723284
32	0.003966271437887808
33	0.004423918142259478
34	0.002974703578415856
35	0.003432350282787526
36	0.008619012932333121
37	0.00404254588861642
38	0.008771561833790345
39	0.004576467043716702
40	0.002059410169672516
41	0.0026696057755014094
42	0.001983135718943904
43	0.001525489014572234
44	0.001449214563843622
45	0.0010678423102005636
46	9.91567859471952E-4
47	0.001525489014572234
48	0.0019068612682152924
49	3.050978029144468E-4
50	0.0012966656623863988
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1311055.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.01696698799692
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.40149540939963	49.05855391180923
2	12.024989586548173	14.674567852668332
3	3.3488094120074843	6.130025800246622
4	1.3847997004831987	3.3798511043748545
5	0.7331143860969385	2.2366208147451263
6	0.4695138982648685	1.7188988418499935
7	0.2913813680712996	1.2445445121566707
8	0.22039493065183816	1.0758264166324063
9	0.17906842863842656	0.9833591158941021
>10	0.8353917562653785	8.892312408146415
>50	0.05595622896504752	2.398818408312584
>100	0.05068315354462125	6.275677695002255
>500	0.0040244489721043015	1.6917526520282327
>1k	3.7729209113477826E-4	0.23919046613317613
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	7.62744507286117E-5
3	0.0	0.0	0.0	0.0	7.62744507286117E-5
4	0.0	0.0	0.0	0.0	7.62744507286117E-5
5	0.0	0.0	0.0	0.0	1.525489014572234E-4
6	0.0	0.0	0.0	0.0	1.525489014572234E-4
7	0.0	0.0	0.0	0.0	1.525489014572234E-4
8	0.0	0.0	0.0	0.0	1.525489014572234E-4
9	0.0	0.0	0.0	0.0	1.525489014572234E-4
10	0.0	0.0	0.0	7.62744507286117E-5	1.525489014572234E-4
11	0.0	0.0	0.0	7.62744507286117E-5	2.2882335218583506E-4
12	0.0	0.0	0.0	7.62744507286117E-5	4.576467043716701E-4
13	0.0	0.0	0.0	7.62744507286117E-5	6.101956058288936E-4
14	0.0	0.0	0.0	7.62744507286117E-5	6.101956058288936E-4
15	0.0	0.0	0.0	7.62744507286117E-5	7.627445072861169E-4
16	0.0	0.0	0.0	1.525489014572234E-4	7.627445072861169E-4
17	0.0	0.0	0.0	1.525489014572234E-4	7.627445072861169E-4
18	0.0	0.0	0.0	2.2882335218583506E-4	8.390189580147286E-4
19	0.0	0.0	0.0	2.2882335218583506E-4	8.390189580147286E-4
20	0.0	0.0	0.0	2.2882335218583506E-4	0.0010678423102005636
21	0.0	0.0	0.0	2.2882335218583506E-4	0.0010678423102005636
22	0.0	0.0	0.0	3.050978029144468E-4	0.0010678423102005636
23	0.0	0.0	0.0	6.864700565575052E-4	0.0011441167609291753
24	0.0	0.0	0.0	8.390189580147286E-4	0.0011441167609291753
25	0.0	0.0	0.0	0.0011441167609291753	0.0011441167609291753
26	0.0	0.0	0.0	0.001449214563843622	0.0011441167609291753
27	0.0	0.0	0.0	0.002059410169672516	0.0011441167609291753
28	0.0	0.0	0.0	0.0033560758320589144	0.0011441167609291753
29	0.0	0.0	0.0	0.0069409750163036636	0.0011441167609291753
30	0.0	0.0	0.0	0.013805675581878715	0.0011441167609291753
31	0.0	0.0	0.0	0.029289389079786888	0.0011441167609291753
32	0.0	0.0	0.0	0.04797662950829675	0.0012203912116577871
33	0.0	0.0	0.0	0.06628249768316356	0.0013729401131150104
34	0.0	0.0	0.0	0.08374934690001563	0.001449214563843622
35	0.0	0.0	0.0	0.10625030986495608	0.001449214563843622
36	0.0	0.0	0.0	0.1369889135085866	0.001449214563843622
37	0.0	0.0	0.0	0.1846604452139689	0.001449214563843622
38	0.0	0.0	0.0	0.25666352670177833	0.001449214563843622
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTCGATC	40	7.0299936E-4	27.501644	27
TAACGGC	110	5.2514224E-9	22.001316	36
GTATCAA	1905	0.0	21.48324	1
ACAATCG	60	0.0074115	18.33373	23
TTAACGG	135	5.5228156E-8	17.926998	35
TAGGACG	210	0.0	17.807873	4
CGGTCCA	300	0.0	17.600382	10
CTAGCGG	290	0.0	17.448654	29
GTATAAA	245	0.0	17.063547	1
GGTATCA	955	0.0	17.049438	1
CGCAATA	285	0.0	16.983473	36
GTCTATA	130	7.307535E-7	16.92538	1
TAGCGGC	295	0.0	16.407135	30
CGGGTAA	135	1.086235E-6	16.295406	44
GTATAGA	150	1.8350693E-7	16.135529	1
AATACGA	315	0.0	16.063229	39
CGACGGT	290	0.0	15.929557	7
AACGCAG	2505	0.0	15.894733	6
ACGGTAT	305	0.0	15.867986	9
CGTCTTA	125	9.436048E-6	15.840947	15
>>END_MODULE