FastQCFastQC Report
Thu 2 Feb 2017
SRR4063942_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063942_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2023214
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT29320.14491793749944396No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA30650.014.0698381
ACCGTTA1650.013.8183268
GGTATCA8850.013.5235581
CGAATGA1450.013.10520614
ACGCGAG751.4781943E-512.66742413
GCGTTAT905.416714E-712.664921
GTAGGAC8250.012.5479223
GACCGCG1301.9826984E-1012.4225927
TAGGACC7100.012.3081614
ACGACGT1405.2750693E-1112.2132056
CGAAATT1501.4551915E-1112.03405413
GCGAAAT1459.640644E-1111.79410112
GGACCGT2150.011.4873546
TGTAGGA9550.011.4365152
CTTACAC1751.8189894E-1211.3967363
GTCCTAA5700.011.3317691
GTCTTAC1604.5474735E-1111.2796941
TAGGACG5750.011.2332334
TATACTG1950.011.2035825
CACGCGA855.319879E-511.17741712
Produced by FastQC (version 0.11.5)