##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063942_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2023214
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.292995204659515	32.0	32.0	32.0	32.0	32.0
2	30.928326909560727	32.0	32.0	32.0	32.0	32.0
3	30.93374601006122	32.0	32.0	32.0	32.0	32.0
4	30.97147113454138	32.0	32.0	32.0	32.0	32.0
5	30.866356203545447	32.0	32.0	32.0	32.0	32.0
6	34.529334020029516	36.0	36.0	36.0	32.0	36.0
7	34.49214319394785	36.0	36.0	36.0	32.0	36.0
8	34.44357789141436	36.0	36.0	36.0	32.0	36.0
9	34.59211680029893	36.0	36.0	36.0	32.0	36.0
10	34.29617183352824	36.0	36.0	36.0	32.0	36.0
11	34.55434669787773	36.0	36.0	36.0	32.0	36.0
12	34.39562053247951	36.0	36.0	36.0	32.0	36.0
13	34.46215575811555	36.0	36.0	36.0	32.0	36.0
14	34.37280435979585	36.0	36.0	36.0	32.0	36.0
15	34.30293236405046	36.0	36.0	36.0	32.0	36.0
16	34.30157709466226	36.0	36.0	36.0	32.0	36.0
17	34.25212854398991	36.0	36.0	36.0	32.0	36.0
18	34.23416455204442	36.0	36.0	36.0	32.0	36.0
19	34.24752695463752	36.0	36.0	36.0	32.0	36.0
20	34.22774407452697	36.0	36.0	36.0	32.0	36.0
21	34.21589955387814	36.0	36.0	36.0	32.0	36.0
22	34.19461806808375	36.0	36.0	36.0	32.0	36.0
23	34.13242741499416	36.0	36.0	36.0	32.0	36.0
24	34.12006984925964	36.0	36.0	36.0	32.0	36.0
25	33.724056377624905	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	3.0
4	19.0
5	25.0
6	83.0
7	34.0
8	109.0
9	112.0
10	182.0
11	65.0
12	127.0
13	101.0
14	151.0
15	314.0
16	524.0
17	639.0
18	859.0
19	1084.0
20	1603.0
21	2337.0
22	3767.0
23	5840.0
24	9027.0
25	13567.0
26	20062.0
27	27182.0
28	37844.0
29	52141.0
30	70245.0
31	96229.0
32	136985.0
33	194256.0
34	417904.0
35	929793.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.26933583851469	17.29895301548845	11.180509029777872	26.25120211621899
2	16.436683035813584	19.335579045185273	38.338206182050946	25.8895317369502
3	19.13686118939844	23.42823701767619	28.584590351596056	28.850311441329318
4	12.646103551069455	15.411264783353772	36.159785521198025	35.78284614437875
5	14.30341059237645	36.67964890132134	34.014031490528275	15.002909015773936
6	33.720868154455005	36.05868238490369	16.933075229441243	13.287374231200054
7	30.268940798417994	30.579310344827586	20.3425040168088	18.80924483994562
8	27.937047896404927	33.96382676799391	18.94862273731885	19.150502598282316
9	27.637217600802284	14.106781338979916	18.44230071218548	39.81370034803231
10	15.80909444290034	27.32358535449099	32.03475128410812	24.832568918500552
11	36.787737470296896	21.479752434974618	22.535291368605332	19.197218726123154
12	24.39249960681987	24.01682712042786	29.430793867774756	22.159879404977513
13	29.892988329461616	20.28592497560498	24.994547286279587	24.826539408653815
14	23.154117609237016	19.79909637883835	25.39323842096282	31.65354759096181
15	25.139539093201137	27.654754121385906	22.16542516520657	25.04028162020639
16	24.970003313731212	26.641113018017798	24.031475500645435	24.357408167605556
17	23.742031908469507	26.304333741680765	25.366437006267645	24.58719734358208
18	23.911317627308282	26.038155263401308	26.422982390449164	23.627544718841246
19	25.633664742446104	24.97364934010825	25.46366527168507	23.929020645760573
20	25.689375356454065	24.97189175766911	25.24661798363613	24.092114902240695
21	26.33654560985852	24.310056323245075	24.73321023701071	24.620187829885694
22	25.798382633066247	25.025510948562115	25.0033017078362	24.172804710535438
23	24.777525279756706	25.300483087963954	25.45308664815308	24.46890498412626
24	24.749244940134364	25.396573368670033	25.20198407869836	24.652197612497243
25	24.586051973323322	25.23135335716487	25.344921652220837	24.83767301729097
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	65.0
1	65.0
2	177.5
3	290.0
4	290.0
5	290.0
6	875.0
7	1460.0
8	1460.0
9	1460.0
10	1993.5
11	2527.0
12	2527.0
13	2527.0
14	3629.0
15	4731.0
16	4731.0
17	4731.0
18	8364.5
19	11998.0
20	11998.0
21	11998.0
22	19477.5
23	26957.0
24	26957.0
25	26957.0
26	40395.5
27	53834.0
28	53834.0
29	53834.0
30	71936.5
31	90039.0
32	90039.0
33	90039.0
34	109521.0
35	129003.0
36	129003.0
37	129003.0
38	152804.0
39	176605.0
40	176605.0
41	176605.0
42	205697.5
43	234790.0
44	234790.0
45	234790.0
46	253533.5
47	272277.0
48	272277.0
49	272277.0
50	277708.0
51	283139.0
52	283139.0
53	283139.0
54	263201.5
55	243264.0
56	243264.0
57	243264.0
58	217293.5
59	191323.0
60	191323.0
61	191323.0
62	164583.0
63	137843.0
64	137843.0
65	137843.0
66	110942.0
67	84041.0
68	84041.0
69	84041.0
70	62901.0
71	41761.0
72	41761.0
73	41761.0
74	30900.5
75	20040.0
76	20040.0
77	20040.0
78	14843.5
79	9647.0
80	9647.0
81	9647.0
82	6787.5
83	3928.0
84	3928.0
85	3928.0
86	2838.0
87	1748.0
88	1748.0
89	1748.0
90	1159.0
91	570.0
92	570.0
93	570.0
94	448.0
95	326.0
96	326.0
97	326.0
98	667.0
99	1008.0
100	1008.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03652604222786122
2	0.004893204574503735
3	0.0019770523533348423
4	0.0038058257801695714
5	0.009539277604840614
6	0.012653135061342993
7	0.022933807298684175
8	0.035784647595360645
9	0.04883319312737061
10	0.05871845489404483
11	0.055308039584542217
12	0.06128862295338011
13	0.06351280685088181
14	0.06667609061621757
15	0.05931157060004527
16	0.06544043289538329
17	0.061436901879880226
18	0.07547397358855762
19	0.07132216364655444
20	0.07735217332422571
21	0.07369462647055625
22	0.07577053144155785
23	0.08086144125139505
24	0.07512798942672401
25	0.07527626835322412
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2023214.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.5574816688719
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.50595771979584	33.264767861875
2	16.214282771117897	15.746494768649255
3	6.520977839678096	9.499267857398673
4	3.122143982369319	6.064137967659077
5	1.7133020658738174	4.159681682845413
6	1.0271662127568362	2.992596272809479
7	0.6421749327032068	2.1827678266061508
8	0.4292978492916132	1.6676497957970908
9	0.3171440974011986	1.3859746826354662
>10	1.3347913197289303	11.389042435145747
>50	0.09847694439532251	3.330174340790671
>100	0.06916269289433638	6.348908892096467
>500	0.004404551914484713	1.4576753067126702
>1k	7.170200791021616E-4	0.5108603089789409
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2932	0.14491793749944396	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.9426308833371065E-5
2	0.0	0.0	0.0	0.0	4.9426308833371065E-5
3	0.0	0.0	0.0	0.0	4.9426308833371065E-5
4	0.0	0.0	0.0	0.0	4.9426308833371065E-5
5	0.0	0.0	0.0	0.0	4.9426308833371065E-5
6	0.0	0.0	0.0	0.0	4.9426308833371065E-5
7	0.0	0.0	0.0	0.0	4.9426308833371065E-5
8	0.0	0.0	0.0	0.0	4.9426308833371065E-5
9	0.0	0.0	0.0	0.0	4.9426308833371065E-5
10	0.0	0.0	0.0	0.0	4.9426308833371065E-5
11	0.0	0.0	0.0	4.9426308833371065E-5	4.9426308833371065E-5
12	0.0	0.0	0.0	4.9426308833371065E-5	1.4827892650011318E-4
13	0.0	0.0	0.0	4.9426308833371065E-5	2.4713154416685534E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3065	0.0	14.069838	1
ACCGTTA	165	0.0	13.818326	8
GGTATCA	885	0.0	13.523558	1
CGAATGA	145	0.0	13.105206	14
ACGCGAG	75	1.4781943E-5	12.667424	13
GCGTTAT	90	5.416714E-7	12.66492	1
GTAGGAC	825	0.0	12.547922	3
GACCGCG	130	1.9826984E-10	12.422592	7
TAGGACC	710	0.0	12.308161	4
ACGACGT	140	5.2750693E-11	12.213205	6
CGAAATT	150	1.4551915E-11	12.034054	13
GCGAAAT	145	9.640644E-11	11.794101	12
GGACCGT	215	0.0	11.487354	6
TGTAGGA	955	0.0	11.436515	2
CTTACAC	175	1.8189894E-12	11.396736	3
GTCCTAA	570	0.0	11.331769	1
GTCTTAC	160	4.5474735E-11	11.279694	1
TAGGACG	575	0.0	11.233233	4
TATACTG	195	0.0	11.203582	5
CACGCGA	85	5.319879E-5	11.177417	12
>>END_MODULE