FastQCFastQC Report
Thu 2 Feb 2017
SRR4063942_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063942_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2023214
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA30850.029.0959361
GGTATCA12400.026.6131881
TAAACGT550.00448500319.999724
TCAACGC45000.019.604174
ATCAACG45200.019.565133
TCGGGTA2700.019.55528325
AGGTATA4900.019.3058517
GGTATAA4900.019.3058518
CAACGCA46050.019.157175
TATCAAC46500.019.1122932
AACGCAG47700.018.540626
CCGTTAT2500.018.4811129
ACCGTTA2500.018.479748
TAGACGT1203.1650416E-718.3330764
CCTTATA4650.017.976912
CGAAATT2950.017.89805413
GTCCTAA8050.017.7641641
GTATAGG5100.017.2550871
TTAGGAC8950.017.2056123
CGCACGA2950.017.15230242
Produced by FastQC (version 0.11.5)