##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063942_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2023214
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.260359012936842	32.0	32.0	32.0	32.0	32.0
2	31.366660175344773	32.0	32.0	32.0	32.0	32.0
3	31.449654856085417	32.0	32.0	32.0	32.0	32.0
4	31.540517216666156	32.0	32.0	32.0	32.0	32.0
5	31.480723739554985	32.0	32.0	32.0	32.0	32.0
6	35.08497667572486	36.0	36.0	36.0	36.0	36.0
7	35.113203052173425	36.0	36.0	36.0	36.0	36.0
8	35.04313137414035	36.0	36.0	36.0	36.0	36.0
9	35.164311832559484	36.0	36.0	36.0	36.0	36.0
10	35.01189295843149	36.0	36.0	36.0	36.0	36.0
11	35.160935521403076	36.0	36.0	36.0	36.0	36.0
12	35.0768406110278	36.0	36.0	36.0	36.0	36.0
13	35.11508174617218	36.0	36.0	36.0	36.0	36.0
14	35.0665115010078	36.0	36.0	36.0	36.0	36.0
15	35.02834104548506	36.0	36.0	36.0	36.0	36.0
16	35.05575139357478	36.0	36.0	36.0	36.0	36.0
17	35.025764452005575	36.0	36.0	36.0	36.0	36.0
18	35.01936028517003	36.0	36.0	36.0	36.0	36.0
19	35.01273666552327	36.0	36.0	36.0	36.0	36.0
20	35.00888240196044	36.0	36.0	36.0	36.0	36.0
21	35.00273920603554	36.0	36.0	36.0	36.0	36.0
22	34.9851375089338	36.0	36.0	36.0	36.0	36.0
23	34.941874166548864	36.0	36.0	36.0	36.0	36.0
24	34.91322272384434	36.0	36.0	36.0	36.0	36.0
25	34.88610201392438	36.0	36.0	36.0	32.0	36.0
26	34.831699464317666	36.0	36.0	36.0	32.0	36.0
27	34.81950352261303	36.0	36.0	36.0	32.0	36.0
28	34.808216036464756	36.0	36.0	36.0	32.0	36.0
29	34.784294691515576	36.0	36.0	36.0	32.0	36.0
30	34.76755746055533	36.0	36.0	36.0	32.0	36.0
31	34.7583354998532	36.0	36.0	36.0	32.0	36.0
32	34.715606950129846	36.0	36.0	36.0	32.0	36.0
33	34.703451537998454	36.0	36.0	36.0	32.0	36.0
34	34.696281263375994	36.0	36.0	36.0	32.0	36.0
35	34.66512538960288	36.0	36.0	36.0	32.0	36.0
36	34.645627699294295	36.0	36.0	36.0	32.0	36.0
37	34.63025315166858	36.0	36.0	36.0	32.0	36.0
38	34.60928255735676	36.0	36.0	36.0	32.0	36.0
39	34.58959902412696	36.0	36.0	36.0	32.0	36.0
40	34.58596273058609	36.0	36.0	36.0	32.0	36.0
41	34.563419391127184	36.0	36.0	36.0	32.0	36.0
42	34.53229465592864	36.0	36.0	36.0	32.0	36.0
43	34.51929207686384	36.0	36.0	36.0	32.0	36.0
44	34.47163226430818	36.0	36.0	36.0	32.0	36.0
45	34.44637888033594	36.0	36.0	36.0	32.0	36.0
46	34.42271702350814	36.0	36.0	36.0	32.0	36.0
47	34.401777073507795	36.0	36.0	36.0	32.0	36.0
48	34.362566194184105	36.0	36.0	36.0	32.0	36.0
49	34.34457452350567	36.0	36.0	36.0	32.0	36.0
50	33.74649196773055	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	1.0
18	0.0
19	3.0
20	17.0
21	56.0
22	179.0
23	582.0
24	1426.0
25	3331.0
26	6892.0
27	12787.0
28	21266.0
29	33632.0
30	49306.0
31	70626.0
32	103314.0
33	167339.0
34	350915.0
35	1201541.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.353703777849944	18.281942572251804	11.809074412675251	26.555279237222994
2	15.841061998691186	20.140677855520252	37.67796030454665	26.340299841241908
3	18.470598702062563	24.00981608434122	28.878020571270408	28.641564642325807
4	12.329244459557911	16.102399449588624	36.18159028160145	35.38676580925201
5	13.975338248944503	37.14135034652785	33.69045489009071	15.192856514436931
6	33.79688712491536	36.44416545983857	16.688233055392175	13.070714359853897
7	30.26779681625217	30.705961260628516	20.346498366706818	18.679743556412497
8	27.97069415296652	34.197717097647605	18.75861871260282	19.07297003678306
9	27.690646184110694	13.97257294809916	18.254484883982432	40.08229598380771
10	15.671008466286839	27.588460068969916	32.104719204863976	24.63581225987927
11	37.09241830078282	21.36654847188681	22.45536062917714	19.085672598153238
12	24.43642859155215	24.08055899205867	29.38524020950834	22.097772206880837
13	30.16008272027932	20.25302441078606	24.877570427895556	24.709322441039063
14	23.2059220684733	19.760064689216946	25.33155200740209	31.702461234907663
15	25.265816127554174	27.708887657560716	22.115516267289603	24.90977994759551
16	25.115114669829975	26.683323448003165	23.945778964017396	24.255782918149464
17	23.823975120773184	26.374570361810466	25.2387537848196	24.56270073259675
18	24.043202676146002	26.081846256044855	26.374796857268258	23.500154210540884
19	25.7820110883036	24.950956720819807	25.48357488806171	23.783457302814877
20	25.875580330377456	24.872911786817028	25.249295547006945	24.00221233579857
21	26.473373553168372	24.321747477034066	24.756056452752897	24.448822517044665
22	25.956054036848336	25.11872211117767	24.96016235569975	23.96506149627424
23	24.91597419518107	25.27461610092587	25.328244335177946	24.481165368715114
24	24.858998609633122	25.391519503441813	25.203352894686503	24.54612899223856
25	24.70849094420217	25.187885006714676	25.369776969808406	24.733847079274746
26	24.583684690767562	25.372966122388053	25.659901161876835	24.38344802496755
27	25.101873966925808	25.32672824909248	25.252337021424676	24.31906076255704
28	24.534334950630903	25.415841212221867	25.467147901665143	24.582675935482083
29	24.797248270530716	25.165137378877837	25.028816250190296	25.00879810040115
30	24.40572927787927	25.645725086399835	25.578305535125388	24.370240100595506
31	25.24780542732565	24.993191004315744	25.16090731906484	24.598096249293768
32	24.690174198392313	25.611656656713556	25.01531026076624	24.682858884127892
33	24.50988000259005	25.296780676645607	25.392078697975958	24.801260622788387
34	24.677830720259397	25.04918048992665	25.71477292947666	24.55821586033729
35	24.843054070659157	25.196908383910426	25.567419898386127	24.392617647044286
36	24.56438406271703	25.29481403125471	25.458576262138198	24.682225643890057
37	24.95173358719539	24.87240169518166	25.105256862332233	25.07060785529072
38	24.738304457066707	25.208187411427357	25.527160134390677	24.52634799711526
39	25.333517206472727	24.838097723630764	25.169267087597756	24.65911798229875
40	25.164025097297998	24.99453826329624	25.449320520412066	24.392116118993695
41	24.77029575240529	25.0939502606091	25.682489391516672	24.453264595468934
42	25.018634068415203	25.261717220844847	25.407626424987246	24.3120222857527
43	24.502467397923272	24.806641412743478	25.736265213643446	24.954625975689805
44	24.20210084282825	24.99352507033454	25.76755101354891	25.036823073288296
45	24.393211921348126	25.105254365127617	25.655967742094926	24.845565971429334
46	24.57002371993181	24.848049592895983	25.733135691948323	24.848790995223887
47	24.59759990865833	24.93395275638811	25.650797040094943	24.81765029485862
48	24.280928288561874	25.46580504595772	25.33803488001961	24.915231785460794
49	24.595864187828624	24.950301649955566	25.521029494772158	24.93280466744365
50	24.02800726971133	25.444681638739635	25.46232704903002	25.06498404251901
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	6.0
1	15.0
2	24.0
3	64.0
4	104.0
5	145.0
6	186.0
7	191.0
8	196.0
9	265.5
10	335.0
11	528.5
12	722.0
13	1238.5
14	1755.0
15	2317.0
16	2879.0
17	2950.5
18	3022.0
19	3109.0
20	3196.0
21	3888.0
22	4580.0
23	5871.5
24	7163.0
25	9118.5
26	11074.0
27	14263.0
28	17452.0
29	21351.5
30	25251.0
31	30417.0
32	35583.0
33	42543.5
34	49504.0
35	58029.5
36	66555.0
37	74330.5
38	82106.0
39	88667.5
40	95229.0
41	103430.5
42	111632.0
43	117155.0
44	122678.0
45	130252.0
46	137826.0
47	143013.0
48	148200.0
49	152857.0
50	157514.0
51	160661.5
52	163809.0
53	159645.5
54	155482.0
55	149972.5
56	144463.0
57	135076.0
58	125689.0
59	114850.5
60	104012.0
61	91226.0
62	78440.0
63	66973.0
64	55506.0
65	46998.5
66	38491.0
67	31618.0
68	24745.0
69	20827.0
70	16909.0
71	13614.5
72	10320.0
73	8910.0
74	7500.0
75	6123.0
76	4746.0
77	3986.0
78	3226.0
79	2788.5
80	2351.0
81	1820.5
82	1290.0
83	985.0
84	680.0
85	498.0
86	316.0
87	252.0
88	188.0
89	147.0
90	106.0
91	84.5
92	63.0
93	53.0
94	43.0
95	33.0
96	23.0
97	22.5
98	22.0
99	22.0
100	22.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009143867134173646
2	4.942630883337106E-4
3	1.9770523533348426E-4
4	0.0
5	0.0
6	1.9770523533348426E-4
7	4.9426308833371065E-5
8	0.0
9	0.001433362956167761
10	6.425420148338238E-4
11	0.0
12	4.9426308833371065E-5
13	4.942630883337106E-4
14	9.885261766674213E-5
15	0.005189762427503962
16	6.919683236671949E-4
17	0.0
18	2.9655785300022636E-4
19	4.9426308833371065E-5
20	6.425420148338238E-4
21	0.0
22	9.885261766674213E-5
23	0.0012850840296676476
24	3.4598416183359744E-4
25	0.0017299208091679872
26	0.005338041354004074
27	0.006227714913004755
28	0.004250662559669912
29	0.0024713154416685533
30	0.0030644311476690053
31	0.007661077869172514
32	0.003015004838835635
33	0.004300088868503283
34	0.002669020677002037
35	0.0032127100741691193
36	0.007858783104505999
37	0.0033609890006692325
38	0.007759930486839256
39	0.0039541047066696845
40	0.002372462824001811
41	0.003410415309502603
42	0.0018781997356681003
43	0.001482789265001132
44	0.0012850840296676476
45	9.885261766674211E-4
46	0.0010379524855007923
47	0.002026478662168214
48	0.0024713154416685533
49	3.954104706669685E-4
50	0.0012356577208342766
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2023214.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.761465201330005
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.64222089592185	37.27187079544848
2	15.340534590930982	16.187781639784085
3	6.126641120965518	9.697516869145788
4	2.8731811825142426	6.063729959133656
5	1.5659280302652425	4.131032863831342
6	0.9315818438549216	2.9490973822045374
7	0.5845220213154004	2.15881668009305
8	0.38422732707326746	1.6217917397420993
9	0.2675730509623533	1.2705791595447513
>10	1.1604393926907497	10.514355133872861
>50	0.0730576347937739	2.6932851549671732
>100	0.04801846702685634	4.700555184287046
>500	0.001885856077204953	0.6245150752405276
>1k	1.8858560772049316E-4	0.11507236270463848
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.4827892650011318E-4
2	0.0	0.0	0.0	0.0	1.4827892650011318E-4
3	0.0	0.0	0.0	0.0	1.4827892650011318E-4
4	0.0	0.0	0.0	0.0	1.4827892650011318E-4
5	0.0	0.0	0.0	0.0	1.4827892650011318E-4
6	0.0	0.0	0.0	0.0	1.4827892650011318E-4
7	0.0	0.0	0.0	0.0	1.4827892650011318E-4
8	0.0	0.0	0.0	0.0	1.4827892650011318E-4
9	0.0	0.0	0.0	0.0	1.4827892650011318E-4
10	0.0	0.0	0.0	0.0	1.4827892650011318E-4
11	0.0	0.0	0.0	4.9426308833371065E-5	1.4827892650011318E-4
12	0.0	0.0	0.0	4.9426308833371065E-5	3.4598416183359744E-4
13	0.0	0.0	0.0	4.9426308833371065E-5	3.954104706669685E-4
14	0.0	0.0	0.0	9.885261766674213E-5	3.954104706669685E-4
15	0.0	0.0	0.0	9.885261766674213E-5	5.931157060004527E-4
16	0.0	0.0	0.0	1.4827892650011318E-4	5.931157060004527E-4
17	0.0	0.0	0.0	1.4827892650011318E-4	5.931157060004527E-4
18	0.0	0.0	0.0	1.9770523533348426E-4	6.425420148338238E-4
19	0.0	0.0	0.0	1.9770523533348426E-4	6.919683236671949E-4
20	0.0	0.0	0.0	3.4598416183359744E-4	8.40247250167308E-4
21	0.0	0.0	0.0	3.954104706669685E-4	9.885261766674213E-4
22	0.0	0.0	0.0	4.4483677950033954E-4	0.0011368051031675345
23	0.0	0.0	0.0	6.919683236671949E-4	0.0011368051031675345
24	0.0	0.0	0.0	6.919683236671949E-4	0.0011368051031675345
25	0.0	0.0	0.0	9.885261766674213E-4	0.0011368051031675345
26	0.0	4.9426308833371065E-5	0.0	0.0011368051031675345	0.0012356577208342766
27	0.0	4.9426308833371065E-5	0.0	0.0020264786621682135	0.0013345103385010186
28	0.0	4.9426308833371065E-5	0.0	0.005733451824671043	0.0013345103385010186
29	0.0	4.9426308833371065E-5	0.0	0.014580761105844463	0.0013345103385010186
30	0.0	4.9426308833371065E-5	0.0	0.033708742624359064	0.0013345103385010186
31	0.0	4.9426308833371065E-5	0.0	0.07077847424938737	0.0013345103385010186
32	0.0	4.9426308833371065E-5	0.0	0.11234599997825243	0.0013839366473343898
33	0.0	4.9426308833371065E-5	0.0	0.15430893617778446	0.0014333629561677607
34	0.0	4.9426308833371065E-5	0.0	0.20279614514332148	0.0014333629561677607
35	0.0	4.9426308833371065E-5	0.0	0.25162933827069206	0.0014333629561677607
36	0.0	4.9426308833371065E-5	0.0	0.3296240536097516	0.0014333629561677607
37	0.0	4.9426308833371065E-5	0.0	0.4481483421921754	0.0014333629561677607
38	0.0	4.9426308833371065E-5	0.0	0.61565410282847	0.001631068191501245
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3085	0.0	29.095936	1
GGTATCA	1240	0.0	26.613188	1
TAAACGT	55	0.004485003	19.99972	4
TCAACGC	4500	0.0	19.60417	4
ATCAACG	4520	0.0	19.56513	3
TCGGGTA	270	0.0	19.555283	25
AGGTATA	490	0.0	19.305851	7
GGTATAA	490	0.0	19.305851	8
CAACGCA	4605	0.0	19.15717	5
TATCAAC	4650	0.0	19.112293	2
AACGCAG	4770	0.0	18.54062	6
CCGTTAT	250	0.0	18.481112	9
ACCGTTA	250	0.0	18.47974	8
TAGACGT	120	3.1650416E-7	18.333076	4
CCTTATA	465	0.0	17.97691	2
CGAAATT	295	0.0	17.898054	13
GTCCTAA	805	0.0	17.764164	1
GTATAGG	510	0.0	17.255087	1
TTAGGAC	895	0.0	17.205612	3
CGCACGA	295	0.0	17.152302	42
>>END_MODULE