FastQCFastQC Report
Thu 2 Feb 2017
SRR4063941_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063941_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences970987
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT28920.29784126872965344No Hit
TATCAACGCAGAGTACTTTTTTTTT21420.22060027580183875No Hit
GTACTTTTTTTTTTTTTTTTTTTTT18030.1856873469984665No Hit
GAGTACTTTTTTTTTTTTTTTTTTT12400.1277051083073203No Hit
ACGCAGAGTACTTTTTTTTTTTTTT11730.12080491293910217No Hit
GGTATCAACGCAGAGTACTTTTTTT10820.11143300579719399No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATT551.1351149E-515.5343252
GCGTTAT508.738841E-515.1938151
CGAACCC456.756166E-414.7786210
CGTTTAT653.3832257E-614.6094381
ACGAACG602.5586882E-514.25448715
GCCGAAC400.00527341114.25154612
GGCGAGA2200.013.82181919
CGATAAC707.2515395E-613.57220310
CGCCAGT1001.0017175E-813.30350218
CGAACGA655.4322925E-513.15798916
TAACGAA655.4401848E-513.15595213
CGGTTCT1301.4551915E-1113.15527312
ATAACGA655.442818E-513.15527312
CCGATAA655.4454522E-513.1545969
CGCCGGT957.384915E-812.9987317
GCGTAAC751.4763602E-512.66738911
GTCCTAA2800.012.5484641
CGCTATT1155.307811E-912.39201111
CGCATCG1001.4372381E-712.35197713
GTACTAT1001.4478974E-712.34497451
Produced by FastQC (version 0.11.5)