##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063941_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	970987
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.2398034165236	32.0	32.0	32.0	32.0	32.0
2	31.000352218927752	32.0	32.0	32.0	32.0	32.0
3	31.023318540824956	32.0	32.0	32.0	32.0	32.0
4	31.031733689534462	32.0	32.0	32.0	32.0	32.0
5	31.006049514566108	32.0	32.0	32.0	32.0	32.0
6	34.5683278972839	36.0	36.0	36.0	32.0	36.0
7	34.515362203613435	36.0	36.0	36.0	32.0	36.0
8	34.48709611972148	36.0	36.0	36.0	32.0	36.0
9	34.57455249143398	36.0	36.0	36.0	32.0	36.0
10	34.39760882483493	36.0	36.0	36.0	32.0	36.0
11	34.552307085470765	36.0	36.0	36.0	32.0	36.0
12	34.44991436548584	36.0	36.0	36.0	32.0	36.0
13	34.492664680371625	36.0	36.0	36.0	32.0	36.0
14	34.43178950902535	36.0	36.0	36.0	32.0	36.0
15	34.3801255835557	36.0	36.0	36.0	32.0	36.0
16	34.37241899222132	36.0	36.0	36.0	32.0	36.0
17	34.32287558947751	36.0	36.0	36.0	32.0	36.0
18	34.31259120873915	36.0	36.0	36.0	32.0	36.0
19	34.312783796281515	36.0	36.0	36.0	32.0	36.0
20	34.25891489793375	36.0	36.0	36.0	32.0	36.0
21	34.23920505629839	36.0	36.0	36.0	32.0	36.0
22	34.228115309473765	36.0	36.0	36.0	32.0	36.0
23	34.19043097384414	36.0	36.0	36.0	32.0	36.0
24	34.15560867447247	36.0	36.0	36.0	32.0	36.0
25	33.69216992606492	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	6.0
5	8.0
6	49.0
7	10.0
8	51.0
9	54.0
10	86.0
11	30.0
12	57.0
13	59.0
14	105.0
15	201.0
16	374.0
17	531.0
18	678.0
19	837.0
20	1136.0
21	1432.0
22	2006.0
23	2836.0
24	4047.0
25	5717.0
26	8171.0
27	11259.0
28	15752.0
29	22145.0
30	30265.0
31	43613.0
32	65493.0
33	98897.0
34	221257.0
35	433822.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.153346884404144	17.05807145528404	11.082275879334343	25.706305780977466
2	16.117609981275837	19.775413054180596	38.51770143922681	25.589275525316758
3	19.02861986325001	24.499443339591686	28.646743613853786	27.825193183304513
4	12.071242007777998	15.40881863423637	36.65216531540439	35.86777404258124
5	13.809071601754253	37.434004395930366	34.298247601704816	14.458676400610567
6	33.67636077106498	36.12963697321461	17.146565176414846	13.047437079305569
7	29.54247894723288	30.578146326388712	21.241070278900878	18.63830444747753
8	27.754574731779886	33.974788236433426	19.383346142513634	18.88729088927305
9	27.46610064707579	13.704818035692206	18.396941845608538	40.43213947162346
10	15.086037671299927	27.58790536732042	32.84156682436032	24.484490137019332
11	37.37542247135438	20.795585689555686	22.708865715934383	19.12012612315555
12	24.264698305266393	23.63280565694371	30.254694368818384	21.847801668971513
13	29.883676354807726	19.855914492640117	25.384585262099186	24.87582389045297
14	22.960305699086735	19.71441703439733	26.116846633126055	31.208430633389877
15	24.738157851348678	27.931168799050322	22.976777352766604	24.3538959968344
16	24.94167281540992	25.994138427103387	24.99917558749696	24.065013169989736
17	23.24959270086983	26.139896559932485	26.044576712897648	24.565934026300035
18	23.830195096311414	25.522678786754476	27.41901905615847	23.228107060775642
19	25.07008112921311	25.010099927445417	25.944549337114964	23.9752696062265
20	25.15490501632532	24.953415124830975	25.969831140133902	23.921848718709803
21	26.136151662089112	24.54986267346171	24.984876045407933	24.32910961904124
22	25.07348363984153	25.216637603011076	25.50418228065185	24.205696476495543
23	23.995906116912728	25.0865775879484	25.955651661259004	24.96186463387987
24	24.416404370706125	25.359330006451756	25.89443437860077	24.32983124424134
25	24.403360720955448	25.145939060135962	25.99941459473053	24.451285624178063
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	85.0
1	85.0
2	265.0
3	445.0
4	445.0
5	445.0
6	1409.0
7	2373.0
8	2373.0
9	2373.0
10	2427.0
11	2481.0
12	2481.0
13	2481.0
14	2600.0
15	2719.0
16	2719.0
17	2719.0
18	4776.5
19	6834.0
20	6834.0
21	6834.0
22	11191.0
23	15548.0
24	15548.0
25	15548.0
26	23792.0
27	32036.0
28	32036.0
29	32036.0
30	41064.5
31	50093.0
32	50093.0
33	50093.0
34	58410.5
35	66728.0
36	66728.0
37	66728.0
38	75097.0
39	83466.0
40	83466.0
41	83466.0
42	93854.0
43	104242.0
44	104242.0
45	104242.0
46	116234.5
47	128227.0
48	128227.0
49	128227.0
50	128172.0
51	128117.0
52	128117.0
53	128117.0
54	118905.0
55	109693.0
56	109693.0
57	109693.0
58	99541.0
59	89389.0
60	89389.0
61	89389.0
62	77571.5
63	65754.0
64	65754.0
65	65754.0
66	53141.5
67	40529.0
68	40529.0
69	40529.0
70	30626.5
71	20724.0
72	20724.0
73	20724.0
74	15689.5
75	10655.0
76	10655.0
77	10655.0
78	8435.5
79	6216.0
80	6216.0
81	6216.0
82	4256.0
83	2296.0
84	2296.0
85	2296.0
86	1729.5
87	1163.0
88	1163.0
89	1163.0
90	800.0
91	437.0
92	437.0
93	437.0
94	294.5
95	152.0
96	152.0
97	152.0
98	368.5
99	585.0
100	585.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.034809940812801816
2	0.00504641153795056
3	0.0018537838302675525
4	0.004428483594528043
5	0.009165931160767343
6	0.01256453484959119
7	0.021833454000928953
8	0.03388304889766804
9	0.04809539159638594
10	0.05561351490802657
11	0.052214911219202735
12	0.05860016663456875
13	0.060865902427117966
14	0.06395554214423056
15	0.05715833476658287
16	0.06168980635168133
17	0.05870315462513917
18	0.07178262942758244
19	0.07034079755959657
20	0.07363641325785
21	0.06920792966332195
22	0.07240055737100497
23	0.07816788484294845
24	0.07301848531442749
25	0.07404836522013168
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	970987.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.10935286559891
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.4956595251166	42.3601260011588
2	14.553499698931091	16.33174900073424
3	4.756965539878895	8.007307741396772
4	1.9946369006550693	4.476711427903993
5	0.9578592508388049	2.687243135044636
6	0.5586814627453606	1.8808353199589032
7	0.33222981639837457	1.3048840000538675
8	0.23235477769058063	1.042982096915886
9	0.154285103016306	0.7791153558342472
>10	0.8182649869889237	8.379387131651992
>50	0.07554371666477565	3.0424914704203463
>100	0.06432211935553402	7.024079844022543
>500	0.004594436870802813	1.617689327682192
>1k	0.0011026648489926752	1.0653981472215968
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2892	0.29784126872965344	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2142	0.22060027580183875	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1803	0.1856873469984665	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1240	0.1277051083073203	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1173	0.12080491293910217	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1082	0.11143300579719399	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.0597598114083918E-4
2	0.0	0.0	0.0	0.0	2.0597598114083918E-4
3	0.0	0.0	0.0	0.0	2.0597598114083918E-4
4	0.0	0.0	0.0	0.0	2.0597598114083918E-4
5	0.0	0.0	0.0	0.0	2.0597598114083918E-4
6	0.0	0.0	0.0	0.0	8.239039245633567E-4
7	0.0	0.0	0.0	0.0	8.239039245633567E-4
8	0.0	0.0	0.0	0.0	8.239039245633567E-4
9	0.0	0.0	0.0	0.0	8.239039245633567E-4
10	0.0	0.0	0.0	0.0	9.268919151337763E-4
11	0.0	0.0	0.0	1.0298799057041959E-4	9.268919151337763E-4
12	0.0	0.0	0.0	1.0298799057041959E-4	0.0014418318679858741
13	0.0	0.0	0.0	1.0298799057041959E-4	0.0017507958396971329
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTATT	55	1.1351149E-5	15.534325	2
GCGTTAT	50	8.738841E-5	15.193815	1
CGAACCC	45	6.756166E-4	14.77862	10
CGTTTAT	65	3.3832257E-6	14.609438	1
ACGAACG	60	2.5586882E-5	14.254487	15
GCCGAAC	40	0.005273411	14.251546	12
GGCGAGA	220	0.0	13.821819	19
CGATAAC	70	7.2515395E-6	13.572203	10
CGCCAGT	100	1.0017175E-8	13.303502	18
CGAACGA	65	5.4322925E-5	13.157989	16
TAACGAA	65	5.4401848E-5	13.155952	13
CGGTTCT	130	1.4551915E-11	13.155273	12
ATAACGA	65	5.442818E-5	13.155273	12
CCGATAA	65	5.4454522E-5	13.154596	9
CGCCGGT	95	7.384915E-8	12.998731	7
GCGTAAC	75	1.4763602E-5	12.667389	11
GTCCTAA	280	0.0	12.548464	1
CGCTATT	115	5.307811E-9	12.392011	11
CGCATCG	100	1.4372381E-7	12.351977	13
GTACTAT	100	1.4478974E-7	12.3449745	1
>>END_MODULE