FastQCFastQC Report
Thu 2 Feb 2017
SRR4063941_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063941_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences970987
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTA1400.023.56977310
ATACCGT1550.022.7069136
GATATAC4200.020.9498311
GTATAGG1057.752169E-820.9498311
GGGTATA856.1178307E-620.7033631
CCGTCGT1653.6379788E-1219.9985949
GGCGTTA1553.45608E-1119.87264342
TATACAG2400.019.2476565
GATTTCG1759.094947E-1218.85873241
ATACACT5050.017.8592134
ACCGTCG1852.1827873E-1117.8356678
TAATACC1254.856229E-717.5978584
CTATACA1751.9099389E-1017.5978584
TAGGACG4500.017.5978584
TACCGTC2003.6379788E-1217.5978587
GTCCTAC4300.017.3932321
GTCGTAG1903.274181E-1117.36720311
TAACGCC2550.017.2528024
AACGCCG2550.017.2528025
CCTTATA1154.276091E-617.2152962
Produced by FastQC (version 0.11.5)