##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063941_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	970987
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.178081683894842	32.0	32.0	32.0	32.0	32.0
2	31.161008334818078	32.0	32.0	32.0	32.0	32.0
3	31.338455612691003	32.0	32.0	32.0	32.0	32.0
4	31.47200219982348	32.0	32.0	32.0	32.0	32.0
5	31.36321701526385	32.0	32.0	32.0	32.0	32.0
6	34.961487640926194	36.0	36.0	36.0	36.0	36.0
7	34.99316468706584	36.0	36.0	36.0	36.0	36.0
8	34.92290936953842	36.0	36.0	36.0	36.0	36.0
9	35.073895942994085	36.0	36.0	36.0	36.0	36.0
10	34.8774617991796	36.0	36.0	36.0	32.0	36.0
11	35.08793938538827	36.0	36.0	36.0	36.0	36.0
12	34.97581018077482	36.0	36.0	36.0	36.0	36.0
13	35.02662342544236	36.0	36.0	36.0	36.0	36.0
14	34.968215846350155	36.0	36.0	36.0	32.0	36.0
15	34.91920180187788	36.0	36.0	36.0	32.0	36.0
16	34.93948837626044	36.0	36.0	36.0	36.0	36.0
17	34.89956508171582	36.0	36.0	36.0	32.0	36.0
18	34.89777206079999	36.0	36.0	36.0	32.0	36.0
19	34.893726692530386	36.0	36.0	36.0	32.0	36.0
20	34.88353294122373	36.0	36.0	36.0	32.0	36.0
21	34.88486766558152	36.0	36.0	36.0	32.0	36.0
22	34.86073140011143	36.0	36.0	36.0	32.0	36.0
23	34.79692519055353	36.0	36.0	36.0	32.0	36.0
24	34.77447895800871	36.0	36.0	36.0	32.0	36.0
25	34.74240437822546	36.0	36.0	36.0	32.0	36.0
26	34.68524192393925	36.0	36.0	36.0	32.0	36.0
27	34.6733344524695	36.0	36.0	36.0	32.0	36.0
28	34.644034369152216	36.0	36.0	36.0	32.0	36.0
29	34.60308016482198	36.0	36.0	36.0	32.0	36.0
30	34.58292129554773	36.0	36.0	36.0	32.0	36.0
31	34.57584087119601	36.0	36.0	36.0	32.0	36.0
32	34.53358180902525	36.0	36.0	36.0	32.0	36.0
33	34.509857495517444	36.0	36.0	36.0	32.0	36.0
34	34.49229495348548	36.0	36.0	36.0	32.0	36.0
35	34.44876192987136	36.0	36.0	36.0	32.0	36.0
36	34.41058016224728	36.0	36.0	36.0	32.0	36.0
37	34.387094780877604	36.0	36.0	36.0	32.0	36.0
38	34.33186850081412	36.0	36.0	36.0	32.0	36.0
39	34.31153043243627	36.0	36.0	36.0	32.0	36.0
40	34.30258592545523	36.0	36.0	36.0	32.0	36.0
41	34.27873699647884	36.0	36.0	36.0	32.0	36.0
42	34.200489810883155	36.0	36.0	36.0	32.0	36.0
43	34.204689661138616	36.0	36.0	36.0	32.0	36.0
44	34.13104706860133	36.0	36.0	36.0	32.0	36.0
45	34.07692070027714	36.0	36.0	36.0	32.0	36.0
46	34.048274590699975	36.0	36.0	36.0	32.0	36.0
47	34.009255530712565	36.0	36.0	36.0	32.0	36.0
48	33.97045377538525	36.0	36.0	36.0	32.0	36.0
49	33.932049553701546	36.0	36.0	36.0	32.0	36.0
50	33.27600163544929	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	2.0
20	10.0
21	23.0
22	93.0
23	289.0
24	735.0
25	1812.0
26	3781.0
27	7072.0
28	11742.0
29	18812.0
30	27747.0
31	39988.0
32	59580.0
33	98670.0
34	198240.0
35	502390.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.02737194890213	18.11311115940432	11.689828523638896	26.16968836805466
2	15.429486552315433	20.67004124677518	37.91304706045923	25.98742514045016
3	18.329138276222885	25.071370897975665	28.899034381617	27.700456444184457
4	11.802732683341796	16.16355316806507	36.701212271637004	35.33250187695613
5	13.603271722484441	37.81955886124119	33.8717202187053	14.70544919756907
6	33.90048878150663	36.508456352614765	16.753382644034005	12.837672221844599
7	29.49888051938957	30.810227953853094	21.117297262782365	18.573594263974968
8	27.744964659671034	34.239284357051126	19.22095764412912	18.794793339148722
9	27.486897244945563	13.456418727902458	18.142410906930014	40.914273120221964
10	14.879281880901893	27.729584821948112	32.90672011089815	24.484413186251842
11	37.50678433387883	20.727774934164927	22.71091168058893	19.054529051367318
12	24.271204734156825	23.682318797593375	30.02030925265658	22.026167215593222
13	30.022739818575793	19.84955467855289	25.324104207690347	24.80360129518097
14	22.9221636563246	19.665989004990802	25.93446249482485	31.477384843859745
15	24.916370392647185	28.072461141070377	22.61006768642605	24.40110077985639
16	24.96294991776375	25.897054840301013	24.905688359116482	24.23430688281875
17	23.464474807592687	26.245459516965724	25.878410318572755	24.411655356868835
18	23.997585946583918	25.58968713085014	27.071075541127993	23.34165138143795
19	25.298175979699007	25.019387489224883	25.761518949275324	23.920917581800786
20	25.33999050446815	24.980972890359563	25.66203527765162	24.017001327520667
21	26.269146754796925	24.440697970209694	24.845337785160872	24.44481748983251
22	25.08334303136911	25.224024626488305	25.410123925449053	24.28250841669353
23	24.076521022683387	25.032673343803907	25.770282448054786	25.120523185457916
24	24.618119351091263	25.36519654288845	25.598671038863667	24.418013067156615
25	24.701227223854037	24.983830635692893	25.8619198081922	24.45302233226087
26	24.025806159243036	25.855905311499995	26.259023555741862	23.85926497351511
27	24.646087835102936	25.415656899085725	25.572825313384133	24.3654299524272
28	23.779813338990976	25.631592715647844	25.933768504919886	24.654825440441297
29	24.010204354874258	25.29223715134949	25.739939317663584	24.957619176112665
30	24.023383188081002	25.773105626013177	25.92213431326304	24.28137687264278
31	24.54612775678401	25.31261490355054	25.4940917044915	24.647165635173955
32	24.217016867737904	25.808318725887414	25.256188167247874	24.718476239126808
33	24.009368183194535	25.303775905148175	25.947787003602674	24.73906890805462
34	24.415092202087614	25.537274773035072	26.00083422162488	24.046798803252432
35	24.6686035673715	25.50601616738777	26.004797279413204	23.82058298582753
36	24.07686635109359	25.98771077686182	25.594068379659955	24.34135449238464
37	24.678617700795705	25.686690107336847	25.266797258801066	24.36789493306638
38	24.119647591248693	25.8739666995565	25.59526139179622	24.411124317398585
39	24.687677017354137	25.211802873474433	25.54621762191668	24.554302487254752
40	25.265946216503753	25.59386673285498	25.548036133050868	23.592150917590402
41	24.13694019551703	25.527928944842444	26.297679419628327	24.037451440012195
42	25.03455307579019	26.120168491302515	25.495947351617453	23.349331081289844
43	24.15116336552088	25.530909105889332	25.820308637906603	24.49761889068319
44	23.885294559895527	25.893072317075433	25.453204720208777	24.768428402820263
45	24.127266285742536	26.1431843554706	25.38363619136288	24.345913167423983
46	24.014937501222995	25.678722196875526	25.378303753222266	24.92803654867922
47	24.51241805832342	25.556945924930353	25.72512912411879	24.205506892627437
48	24.712269004149505	26.011499970647577	24.862841788376233	24.41338923682668
49	24.223979896599314	25.90485900842448	25.2460400832149	24.625121011761316
50	24.37684028181866	26.3937737067434	24.74193571642641	24.487450295011527
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	23.0
1	17.0
2	11.0
3	52.5
4	94.0
5	128.0
6	162.0
7	164.0
8	166.0
9	222.0
10	278.0
11	418.0
12	558.0
13	1021.5
14	1485.0
15	1997.0
16	2509.0
17	2810.5
18	3112.0
19	3349.0
20	3586.0
21	4120.5
22	4655.0
23	5360.0
24	6065.0
25	6783.0
26	7501.0
27	9666.5
28	11832.0
29	13491.0
30	15150.0
31	17340.5
32	19531.0
33	22638.0
34	25745.0
35	30023.5
36	34302.0
37	37506.0
38	40710.0
39	42846.5
40	44983.0
41	48159.0
42	51335.0
43	52597.5
44	53860.0
45	58616.0
46	63372.0
47	66787.0
48	70202.0
49	72333.5
50	74465.0
51	71998.0
52	69531.0
53	67925.0
54	66319.0
55	65483.0
56	64647.0
57	62429.5
58	60212.0
59	54790.0
60	49368.0
61	43602.5
62	37837.0
63	32702.0
64	27567.0
65	23237.0
66	18907.0
67	16111.0
68	13315.0
69	11853.0
70	10391.0
71	8097.5
72	5804.0
73	5036.5
74	4269.0
75	3344.0
76	2419.0
77	2153.5
78	1888.0
79	1557.0
80	1226.0
81	974.0
82	722.0
83	560.5
84	399.0
85	311.0
86	223.0
87	167.0
88	111.0
89	80.0
90	49.0
91	43.0
92	37.0
93	25.5
94	14.0
95	14.0
96	14.0
97	13.0
98	12.0
99	13.0
100	14.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.006797207377647693
2	2.0597598114083915E-4
3	3.0896397171125876E-4
4	0.0
5	0.0
6	1.0298799057041958E-4
7	1.0298799057041958E-4
8	0.0
9	8.239039245633566E-4
10	6.179279434225175E-4
11	0.0
12	1.0298799057041958E-4
13	3.0896397171125876E-4
14	1.0298799057041958E-4
15	0.004016531632246364
16	4.119519622816783E-4
17	0.0
18	6.179279434225175E-4
19	0.0
20	4.119519622816783E-4
21	0.0
22	0.0
23	0.001235855886845035
24	3.0896397171125876E-4
25	0.0015448198585562936
26	0.0057673274719434965
27	0.005561351490802657
28	0.002986651726542168
29	0.002986651726542168
30	0.0033986036888238465
31	0.006591231396506853
32	0.002162747801978811
33	0.004222507613387203
34	0.0022657357925492307
35	0.0020597598114083914
36	0.005664339481373078
37	0.0025746997642604897
38	0.007106171349358952
39	0.0038105556511055243
40	0.0020597598114083914
41	0.0023687237831196504
42	0.0017507958396971329
43	0.0011328678962746154
44	0.0013388438774154545
45	0.0015448198585562936
46	8.239039245633566E-4
47	0.0022657357925492307
48	0.0028836637359717482
49	7.209159339929371E-4
50	8.239039245633566E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	970987.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.70723976276281
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.13800623262256	46.8283543918531
2	13.881454660572912	16.85409592670642
3	4.403010251968318	8.018837971324302
4	1.7886298896518433	4.343311342317538
5	0.8859872417512046	2.6892919955869634
6	0.48306006390779527	1.7595145871679632
7	0.3073297184456673	1.3059997232732459
8	0.18975086156152582	0.9215400838405089
9	0.13163928610232958	0.7192311933251747
>10	0.6805925915646132	7.4979632004114265
>50	0.06090524886303144	2.615603582896971
>100	0.04690493873627223	5.436491728643757
>500	0.0027290142518407285	1.009764272652688
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.179279434225175E-4
2	0.0	0.0	0.0	0.0	6.179279434225175E-4
3	0.0	0.0	0.0	0.0	6.179279434225175E-4
4	0.0	0.0	0.0	0.0	6.179279434225175E-4
5	0.0	0.0	0.0	0.0	6.179279434225175E-4
6	0.0	0.0	0.0	0.0	6.179279434225175E-4
7	0.0	0.0	0.0	0.0	6.179279434225175E-4
8	0.0	0.0	0.0	0.0	6.179279434225175E-4
9	0.0	0.0	0.0	0.0	6.179279434225175E-4
10	0.0	0.0	0.0	0.0	6.179279434225175E-4
11	0.0	0.0	0.0	1.0298799057041959E-4	6.179279434225175E-4
12	0.0	0.0	0.0	1.0298799057041959E-4	0.001956771820837972
13	0.0	0.0	0.0	1.0298799057041959E-4	0.002162747801978811
14	0.0	0.0	0.0	1.0298799057041959E-4	0.002162747801978811
15	0.0	0.0	0.0	1.0298799057041959E-4	0.0026776877548309094
16	0.0	0.0	0.0	2.0597598114083918E-4	0.0027806757454013286
17	0.0	0.0	0.0	2.0597598114083918E-4	0.002986651726542168
18	0.0	0.0	0.0	3.0896397171125876E-4	0.0030896397171125876
19	0.0	0.0	0.0	5.14939952852098E-4	0.0030896397171125876
20	0.0	0.0	0.0	5.14939952852098E-4	0.003398603688823846
21	0.0	0.0	0.0	8.239039245633567E-4	0.0035015916793942658
22	0.0	0.0	0.0	9.268919151337763E-4	0.0036045796699646854
23	0.0	0.0	0.0	0.002059759811408392	0.0036045796699646854
24	0.0	0.0	0.0	0.0038105556511055247	0.003707567660535105
25	0.0	0.0	0.0	0.00484043555680972	0.003707567660535105
26	0.0	0.0	0.0	0.005973303453084336	0.003707567660535105
27	0.0	0.0	0.0	0.0070031833587885315	0.003707567660535105
28	0.0	0.0	0.0	0.010298799057041959	0.003707567660535105
29	0.0	0.0	0.0	0.015345210594992519	0.003707567660535105
30	0.0	0.0	0.0	0.025746997642604897	0.003707567660535105
31	0.0	0.0	0.0	0.04376989599242832	0.003707567660535105
32	0.0	0.0	0.0	0.0651913980310756	0.003707567660535105
33	0.0	0.0	0.0	0.08300832039975818	0.0038105556511055247
34	0.0	0.0	0.0	0.10442982243840546	0.004325495603957622
35	0.0	0.0	0.0	0.13100072400557372	0.004325495603957622
36	0.0	0.0	0.0	0.1687973165449177	0.004325495603957622
37	0.0	0.0	0.0	0.22060027580183875	0.004325495603957622
38	0.0	0.0	0.0	0.2904261334085832	0.0045314715850984615
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCGTA	140	0.0	23.569773	10
ATACCGT	155	0.0	22.706913	6
GATATAC	420	0.0	20.949831	1
GTATAGG	105	7.752169E-8	20.949831	1
GGGTATA	85	6.1178307E-6	20.703363	1
CCGTCGT	165	3.6379788E-12	19.998594	9
GGCGTTA	155	3.45608E-11	19.872643	42
TATACAG	240	0.0	19.247656	5
GATTTCG	175	9.094947E-12	18.858732	41
ATACACT	505	0.0	17.859213	4
ACCGTCG	185	2.1827873E-11	17.835667	8
TAATACC	125	4.856229E-7	17.597858	4
CTATACA	175	1.9099389E-10	17.597858	4
TAGGACG	450	0.0	17.597858	4
TACCGTC	200	3.6379788E-12	17.597858	7
GTCCTAC	430	0.0	17.393232	1
GTCGTAG	190	3.274181E-11	17.367203	11
TAACGCC	255	0.0	17.252802	4
AACGCCG	255	0.0	17.252802	5
CCTTATA	115	4.276091E-6	17.215296	2
>>END_MODULE