FastQCFastQC Report
Thu 2 Feb 2017
SRR4063940_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063940_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1752527
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT31280.17848512462290167No Hit
TATCAACGCAGAGTACTTTTTTTTT20790.11862870015697333No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGTA402.7589628E-416.626913
CTACCGA400.005284460714.2479654
GCGTTAT1203.6379788E-1214.2471511
GCTACCG400.005295482514.2434913
CGTTATT1051.382432E-913.5644552
GGTATCA7450.013.3865851
CGCATCG3000.013.30151913
GCATCGC3200.013.06473814
GTATCAA21100.012.7841751
AACCGCG1352.7284841E-1112.6670287
TAAGGGA604.0974194E-412.6648584
TCGCCAG3400.012.57496617
ATCGCCA3300.012.38055616
CGGTCCA3400.012.2948210
GTAATAC853.964313E-612.2885033
ACCGTTT550.003067412.09168
TACTCCG550.00307133912.0895275
CATCGCC3750.011.90870815
TCTTATA1157.125345E-811.5592772
CGCCAGT3550.011.24136318
Produced by FastQC (version 0.11.5)