##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063940_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1752527
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.275733840334556	32.0	32.0	32.0	32.0	32.0
2	30.852955760453334	32.0	32.0	32.0	32.0	32.0
3	30.865856560269826	32.0	32.0	32.0	32.0	32.0
4	30.907873601947358	32.0	32.0	32.0	32.0	32.0
5	30.802323730247807	32.0	32.0	32.0	32.0	32.0
6	34.46637056091004	36.0	36.0	36.0	32.0	36.0
7	34.40078754849426	36.0	36.0	36.0	32.0	36.0
8	34.37079885217175	36.0	36.0	36.0	32.0	36.0
9	34.51673840117727	36.0	36.0	36.0	32.0	36.0
10	34.19872903527307	36.0	36.0	36.0	32.0	36.0
11	34.486156276051666	36.0	36.0	36.0	32.0	36.0
12	34.29155841821552	36.0	36.0	36.0	32.0	36.0
13	34.37903552983777	36.0	36.0	36.0	32.0	36.0
14	34.27739544098322	36.0	36.0	36.0	32.0	36.0
15	34.21344721079903	36.0	36.0	36.0	32.0	36.0
16	34.211947661861984	36.0	36.0	36.0	32.0	36.0
17	34.1424331836257	36.0	36.0	36.0	32.0	36.0
18	34.133864984676414	36.0	36.0	36.0	32.0	36.0
19	34.15244272984096	36.0	36.0	36.0	32.0	36.0
20	34.133733175009574	36.0	36.0	36.0	32.0	36.0
21	34.117794476204935	36.0	36.0	36.0	32.0	36.0
22	34.09045167349775	36.0	36.0	36.0	32.0	36.0
23	34.03371132085269	36.0	36.0	36.0	32.0	36.0
24	34.01590788615525	36.0	36.0	36.0	32.0	36.0
25	33.61998987747407	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	10.0
4	15.0
5	28.0
6	56.0
7	29.0
8	98.0
9	131.0
10	172.0
11	46.0
12	113.0
13	104.0
14	120.0
15	253.0
16	456.0
17	569.0
18	768.0
19	1037.0
20	1624.0
21	2412.0
22	3691.0
23	5750.0
24	8684.0
25	13047.0
26	18995.0
27	25424.0
28	34675.0
29	47491.0
30	62871.0
31	85874.0
32	122021.0
33	174714.0
34	373029.0
35	768219.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.187520478211184	17.14067660580571	11.284050912686045	26.387752003297066
2	16.700068020068205	19.46264146119889	37.71923917955928	26.118051339173626
3	18.877190019263953	23.443420127635097	28.5683961618172	29.11099369128375
4	12.780151605415066	15.519240557758193	35.720570814245704	35.98003702258104
5	14.76752845440305	36.376896365768545	33.609778784671	15.245796395157402
6	34.247359446299804	35.14652875903678	16.985146243417162	13.62096555124626
7	30.553297962687314	30.198297825709773	20.62020007853379	18.62820413306912
8	27.97457790625061	33.15997492953212	19.31653292325537	19.5489142409619
9	27.612207848555087	14.159425915988264	18.378737882922486	39.84962835253417
10	16.231389406385084	26.20221993971986	31.45791951876375	26.108471135131307
11	37.014274181669535	20.720343192388015	23.0074550941621	19.25792753178035
12	25.3126944883778	23.13351109690018	28.729694699638575	22.82409971508345
13	29.73887930276457	19.376843552547886	25.042737873235527	25.841539271452014
14	23.5727740894424	19.753248555230147	24.871339683221255	31.802637672106194
15	25.11727466022322	27.641420450782327	21.80132507804988	25.439979810944568
16	25.655227228994498	25.44498809196849	23.99598250253092	24.90380217650609
17	24.0543196314286	25.87308176093447	25.220350905641226	24.852247701995704
18	24.76705347847961	24.65427064339895	26.38998419898204	24.188691679139403
19	25.937171118563775	24.479657103896127	25.03790255944349	24.54526921809661
20	26.08336878652988	24.279510146276557	24.772333372355643	24.86478769483792
21	26.816431065643748	23.941365753100026	24.151222450954922	25.09098073030131
22	26.248966380527293	24.35081844388303	24.506946379704967	24.89326879588471
23	24.593028734270106	24.303030476087137	25.278219577452322	25.825721212190434
24	25.159692504131502	24.62194020253795	25.072037222943642	25.146330070386906
25	25.000913690510178	24.411983051041037	25.230478431193394	25.356624827255388
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	97.0
1	97.0
2	256.0
3	415.0
4	415.0
5	415.0
6	1085.0
7	1755.0
8	1755.0
9	1755.0
10	2002.0
11	2249.0
12	2249.0
13	2249.0
14	3086.0
15	3923.0
16	3923.0
17	3923.0
18	6785.0
19	9647.0
20	9647.0
21	9647.0
22	16245.0
23	22843.0
24	22843.0
25	22843.0
26	35486.0
27	48129.0
28	48129.0
29	48129.0
30	61708.0
31	75287.0
32	75287.0
33	75287.0
34	93161.5
35	111036.0
36	111036.0
37	111036.0
38	127189.0
39	143342.0
40	143342.0
41	143342.0
42	163409.0
43	183476.0
44	183476.0
45	183476.0
46	204560.5
47	225645.0
48	225645.0
49	225645.0
50	231621.0
51	237597.0
52	237597.0
53	237597.0
54	222002.0
55	206407.0
56	206407.0
57	206407.0
58	190301.5
59	174196.0
60	174196.0
61	174196.0
62	152646.5
63	131097.0
64	131097.0
65	131097.0
66	107595.0
67	84093.0
68	84093.0
69	84093.0
70	63802.5
71	43512.0
72	43512.0
73	43512.0
74	33239.5
75	22967.0
76	22967.0
77	22967.0
78	18442.5
79	13918.0
80	13918.0
81	13918.0
82	9698.0
83	5478.0
84	5478.0
85	5478.0
86	4143.0
87	2808.0
88	2808.0
89	2808.0
90	2033.5
91	1259.0
92	1259.0
93	1259.0
94	860.0
95	461.0
96	461.0
97	461.0
98	675.5
99	890.0
100	890.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.037945207120917396
2	0.005877227569104499
3	0.0017688743169149465
4	0.004736018332385178
5	0.009243794817426492
6	0.01386569222613974
7	0.023680091661925893
8	0.037945207120917396
9	0.049471420411782534
10	0.059228759385732715
11	0.05551982936639493
12	0.06487774510749335
13	0.06579071249686881
14	0.06904315882151887
15	0.06179648016835118
16	0.06778782866112762
17	0.06367947540893806
18	0.07840107456261729
19	0.07566217239449093
20	0.07942816287566469
21	0.07600453516550673
22	0.07982758610851644
23	0.08456360444090162
24	0.07697456301671814
25	0.07914286056648485
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1752527.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.17360169626131
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.31975685145433	43.51210516011242
2	11.389697951905546	12.340419205745084
3	3.2937963555173693	5.353104354971851
4	1.4873610344930162	3.2230281704465535
5	0.8201954614499234	2.22164711208347
6	0.5491362685273429	1.784921369290288
7	0.37671939547283606	1.4285772537121237
8	0.273564123400575	1.18559630875917
9	0.20417307336141893	0.9954711678044743
>10	1.1162910558703547	10.874024360955138
>50	0.08156062254730093	3.0675086547101125
>100	0.07580189130566006	8.58025876458545
>500	0.008985865035671874	3.270128240899797
>1k	0.0029600496588095583	2.1632098759241183
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3128	0.17848512462290167	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2079	0.11862870015697333	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	5.706046183596601E-5
7	0.0	0.0	0.0	0.0	5.706046183596601E-5
8	0.0	0.0	0.0	0.0	5.706046183596601E-5
9	0.0	0.0	0.0	0.0	5.706046183596601E-5
10	0.0	0.0	0.0	0.0	5.706046183596601E-5
11	0.0	0.0	0.0	0.0	5.706046183596601E-5
12	0.0	0.0	0.0	0.0	1.7118138550789802E-4
13	0.0	0.0	0.0	0.0	2.2824184734386403E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCGTA	40	2.7589628E-4	16.6269	13
CTACCGA	40	0.0052844607	14.247965	4
GCGTTAT	120	3.6379788E-12	14.247151	1
GCTACCG	40	0.0052954825	14.243491	3
CGTTATT	105	1.382432E-9	13.564455	2
GGTATCA	745	0.0	13.386585	1
CGCATCG	300	0.0	13.301519	13
GCATCGC	320	0.0	13.064738	14
GTATCAA	2110	0.0	12.784175	1
AACCGCG	135	2.7284841E-11	12.667028	7
TAAGGGA	60	4.0974194E-4	12.664858	4
TCGCCAG	340	0.0	12.574966	17
ATCGCCA	330	0.0	12.380556	16
CGGTCCA	340	0.0	12.29482	10
GTAATAC	85	3.964313E-6	12.288503	3
ACCGTTT	55	0.0030674	12.0916	8
TACTCCG	55	0.003071339	12.089527	5
CATCGCC	375	0.0	11.908708	15
TCTTATA	115	7.125345E-8	11.559277	2
CGCCAGT	355	0.0	11.241363	18
>>END_MODULE