FastQCFastQC Report
Thu 2 Feb 2017
SRR4063940_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063940_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1752527
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA23000.025.4418951
GGTATCA10400.020.3064271
TAGGACG10050.019.4824854
GTATTAC1851.8189894E-1219.0258431
GACGTGA6000.018.3332397
GTCTATA1456.31735E-918.2057631
GTATAAT1355.5337296E-817.924811
TTAGGAC7000.017.9131723
ACCGTCG4300.017.9068838
AACGCAG32800.017.7742986
TACCGTC4500.017.599917
AGGACGT11250.017.5999075
TCAACGC33250.017.4675794
GGACGTG11450.017.2924866
GTCCTAC9900.017.1100461
CAACGCA34250.016.9575775
ATACCGT4450.016.80896
TATCAAC34700.016.7362332
GTCCTAA5800.016.6886161
ATCAACG35050.016.5695823
Produced by FastQC (version 0.11.5)