##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063940_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1752527
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.370257918993545	32.0	32.0	32.0	32.0	32.0
2	31.434311711032127	32.0	32.0	32.0	32.0	32.0
3	31.49791529602682	32.0	32.0	32.0	32.0	32.0
4	31.571466231333382	32.0	32.0	32.0	32.0	32.0
5	31.528457478829143	32.0	32.0	32.0	32.0	32.0
6	35.127029711953085	36.0	36.0	36.0	36.0	36.0
7	35.14486909474148	36.0	36.0	36.0	36.0	36.0
8	35.09306732506832	36.0	36.0	36.0	36.0	36.0
9	35.195729366794346	36.0	36.0	36.0	36.0	36.0
10	35.05264341148524	36.0	36.0	36.0	36.0	36.0
11	35.19553422001487	36.0	36.0	36.0	36.0	36.0
12	35.0958204923519	36.0	36.0	36.0	36.0	36.0
13	35.138188455869724	36.0	36.0	36.0	36.0	36.0
14	35.0821145694189	36.0	36.0	36.0	36.0	36.0
15	35.058170858423296	36.0	36.0	36.0	36.0	36.0
16	35.07095240187455	36.0	36.0	36.0	36.0	36.0
17	35.039243903232304	36.0	36.0	36.0	36.0	36.0
18	35.03425396584475	36.0	36.0	36.0	36.0	36.0
19	35.02770913087216	36.0	36.0	36.0	36.0	36.0
20	35.02489890312674	36.0	36.0	36.0	36.0	36.0
21	35.01463429664707	36.0	36.0	36.0	36.0	36.0
22	35.001469877496895	36.0	36.0	36.0	36.0	36.0
23	34.95532394080091	36.0	36.0	36.0	36.0	36.0
24	34.91509175036961	36.0	36.0	36.0	32.0	36.0
25	34.89516794891035	36.0	36.0	36.0	32.0	36.0
26	34.8346148162054	36.0	36.0	36.0	32.0	36.0
27	34.82774816022806	36.0	36.0	36.0	32.0	36.0
28	34.80233685415403	36.0	36.0	36.0	32.0	36.0
29	34.77253417493711	36.0	36.0	36.0	32.0	36.0
30	34.75678776988885	36.0	36.0	36.0	32.0	36.0
31	34.749234105951004	36.0	36.0	36.0	32.0	36.0
32	34.71065267468062	36.0	36.0	36.0	32.0	36.0
33	34.695496845412364	36.0	36.0	36.0	32.0	36.0
34	34.690061265817874	36.0	36.0	36.0	32.0	36.0
35	34.6501189425327	36.0	36.0	36.0	32.0	36.0
36	34.624416628103305	36.0	36.0	36.0	32.0	36.0
37	34.61193750509978	36.0	36.0	36.0	32.0	36.0
38	34.5770484563148	36.0	36.0	36.0	32.0	36.0
39	34.56754503639601	36.0	36.0	36.0	32.0	36.0
40	34.54761895251828	36.0	36.0	36.0	32.0	36.0
41	34.54180449145719	36.0	36.0	36.0	32.0	36.0
42	34.47949446713232	36.0	36.0	36.0	32.0	36.0
43	34.48092782593364	36.0	36.0	36.0	32.0	36.0
44	34.40765477507622	36.0	36.0	36.0	32.0	36.0
45	34.386610876751114	36.0	36.0	36.0	32.0	36.0
46	34.36003953148796	36.0	36.0	36.0	32.0	36.0
47	34.33758338673242	36.0	36.0	36.0	32.0	36.0
48	34.30097339441846	36.0	36.0	36.0	32.0	36.0
49	34.28209494062003	36.0	36.0	36.0	32.0	36.0
50	33.6869697299956	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	2.0
17	5.0
18	2.0
19	4.0
20	22.0
21	59.0
22	143.0
23	435.0
24	1292.0
25	3068.0
26	6039.0
27	11151.0
28	18948.0
29	29328.0
30	42984.0
31	61314.0
32	88343.0
33	143068.0
34	302037.0
35	1044283.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.34994287853385	17.988753747704582	11.832988474132137	26.828314899629426
2	15.971951183327285	20.16415256693805	37.28616457709944	26.57773167263523
3	18.17836342233454	24.03768737985179	28.870034801255105	28.91391439655856
4	12.470392752864862	16.11501563171352	35.789006389059914	35.62558522636171
5	14.37022082969335	36.856322327701655	33.35075579434725	15.422701048257744
6	34.385149647023376	35.460398830486774	16.746247127000828	13.40820439548902
7	30.372217017037123	30.46111726730445	20.65989320557869	18.506772510079735
8	28.033421472777007	33.27733796816709	19.153439093057678	19.535801465998222
9	27.72010791416168	14.015831062297146	18.282925459799234	39.98113556374194
10	16.174143669611592	26.505514671268077	31.461957070642228	25.858384588478106
11	37.29939681385793	20.639567892534608	22.89111665611999	19.169918637487466
12	25.272792114235177	23.302663300095578	28.653799517838547	22.770745067830703
13	29.816013302003174	19.36493662261957	25.019258004767998	25.79979207060926
14	23.666508228422682	19.736859373600232	24.805780009734537	31.790852388242552
15	25.175512328232447	27.761391111027418	21.758507133763704	25.30458942697643
16	25.778979604762746	25.508739715506323	23.850496172077076	24.861784507653848
17	24.21937008673761	25.92273899346487	25.05473524801615	24.803155671781376
18	25.03064152045849	24.639947869472827	26.323747920142605	24.005662689926073
19	26.00029899699063	24.493616642492036	25.054464241899975	24.451620118617356
20	26.318032863589913	24.2585378629708	24.652428478582518	24.771000794856768
21	26.934135679507364	23.931956540469848	24.131725217357562	25.002182562665226
22	26.236490176151957	24.442274871456604	24.476967751028376	24.844267201363067
23	24.64229978060102	24.29611158820324	25.234363186620374	25.82722544457537
24	25.40006698928799	24.608805953031037	25.00138372251599	24.98974333516498
25	25.18636191415255	24.396064145930623	25.164336286821598	25.25323765309523
26	24.721370223377452	25.178282541521273	25.47575774003304	24.624589495068232
27	25.35225814992856	24.812372461207662	24.711482714071153	25.12388667479263
28	24.748267286901683	24.650803958334997	25.25977857336056	25.341150181402767
29	24.954264761098287	24.614003713598688	25.003166944178474	25.428564581124558
30	24.760680366203548	24.833720216289258	25.347852286033184	25.057747131474013
31	25.67674216490157	24.287621055202486	24.518903471404695	25.516733308491258
32	25.072112545214782	24.811280541909728	24.408762722764283	25.707844190111206
33	24.606050853463756	24.628362512532476	25.17160290724912	25.593983726754647
34	25.405782323211863	24.546199982995518	25.32481131014979	24.72320638364283
35	25.788699740309834	24.46354380352793	25.092768904285546	24.65498755187669
36	24.767445509976405	24.885113716449297	25.065039931293636	25.282400842280662
37	25.681215391427802	24.644898359787636	24.53596552495469	25.137920723829872
38	25.12112002209549	24.784551875890923	24.929838837613	25.164489264400586
39	25.76338752787523	24.28431705975399	24.475306598982456	25.476988813388328
40	26.06561782980814	24.501891889270127	25.00146934545938	24.431020935462353
41	25.180945642125923	24.565639158668386	25.445659244718854	24.807755954486836
42	25.835308134621627	25.187432560512228	24.84688989481865	24.130369410047493
43	25.028787347946313	24.387591255037073	25.290526822732705	25.29309457428391
44	24.844665207802546	24.785264521356574	24.847575327887796	25.522494942953088
45	24.96202572094069	25.066733162073422	24.82718992617412	25.144051190811766
46	24.98151216773257	24.48673440973601	24.923252671605887	25.608500750925533
47	25.432213593230202	24.42587404207727	25.16014356715302	24.981768797539498
48	25.671629697385036	25.024607829655043	24.29638725676765	25.00737521619227
49	25.10734548384354	24.95533571961274	24.605895856193285	25.331422940350436
50	24.949515180253204	25.503749771042283	24.463982169543403	25.082752879161106
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	17.0
1	22.5
2	28.0
3	85.5
4	143.0
5	165.5
6	188.0
7	180.0
8	172.0
9	234.5
10	297.0
11	485.0
12	673.0
13	1225.0
14	1777.0
15	2357.5
16	2938.0
17	3078.0
18	3218.0
19	3184.0
20	3150.0
21	3564.5
22	3979.0
23	4655.0
24	5331.0
25	7025.0
26	8719.0
27	11482.5
28	14246.0
29	17345.5
30	20445.0
31	23952.0
32	27459.0
33	33289.0
34	39119.0
35	46397.0
36	53675.0
37	61062.0
38	68449.0
39	72662.5
40	76876.0
41	82973.5
42	89071.0
43	91097.5
44	93124.0
45	101585.5
46	110047.0
47	117625.0
48	125203.0
49	130159.5
50	135116.0
51	132624.0
52	130132.0
53	128780.5
54	127429.0
55	128180.5
56	128932.0
57	125365.5
58	121799.0
59	111343.0
60	100887.0
61	88365.0
62	75843.0
63	66293.5
64	56744.0
65	48181.0
66	39618.0
67	33857.0
68	28096.0
69	25286.0
70	22476.0
71	17684.0
72	12892.0
73	11023.5
74	9155.0
75	7193.5
76	5232.0
77	4592.5
78	3953.0
79	3271.5
80	2590.0
81	2037.5
82	1485.0
83	1206.5
84	928.0
85	684.5
86	441.0
87	304.0
88	167.0
89	132.0
90	97.0
91	83.5
92	70.0
93	48.0
94	26.0
95	34.5
96	43.0
97	30.0
98	17.0
99	16.0
100	15.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0069043158821518865
2	6.276650801956261E-4
3	2.2824184734386403E-4
4	0.0
5	0.0
6	1.7118138550789802E-4
7	5.706046183596601E-5
8	5.706046183596601E-5
9	0.0013123906222272182
10	0.0011412092367193202
11	0.0
12	1.1412092367193202E-4
13	5.135441565236941E-4
14	2.2824184734386403E-4
15	0.004907199717893077
16	5.706046183596601E-4
17	0.0
18	1.7118138550789802E-4
19	5.706046183596601E-5
20	5.706046183596601E-4
21	0.0
22	3.4236277101579605E-4
23	6.847255420315921E-4
24	4.5648369468772806E-4
25	0.0013123906222272182
26	0.006390771725628193
27	0.0067901949584799545
28	0.004165413714025518
29	0.0027959626299623344
30	0.0033665672483219945
31	0.007075497267659786
32	0.0033095067864860285
33	0.004564836946877281
34	0.0027389021681263684
35	0.003081264939142165
36	0.008102585580707172
37	0.004051292790353586
38	0.0075890414241834795
39	0.00427953463769745
40	0.0026247812444544364
41	0.0038230509430097225
42	0.0017118138550789802
43	0.0011982696985552862
44	0.0012553301603912522
45	0.0014265115458991502
46	0.0013123906222272182
47	0.0021112370879307423
48	0.0020541766260947763
49	4.5648369468772806E-4
50	0.0011412092367193202
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1752527.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.24834866684691
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.6125983578983	47.53799084758198
2	10.984066408607903	12.796074598967882
3	2.972093189157974	5.193585610573039
4	1.3223651338031128	3.0810234151458156
5	0.7551099161481877	2.1991952838796585
6	0.4837380684532301	1.6906166204814481
7	0.3288369463474903	1.3407946373772892
8	0.25807721055424193	1.2026057074664598
9	0.18610401965287887	0.9756226642538356
>10	0.9621386620908657	10.015616428211745
>50	0.06698280868406566	2.7328310688074327
>100	0.05931741229319662	7.255850769023654
>500	0.006798376727503833	2.663491544495504
>1k	0.0017734895810879567	1.3147008037341736
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.1412092367193202E-4
2	0.0	0.0	0.0	0.0	1.1412092367193202E-4
3	0.0	0.0	0.0	0.0	1.1412092367193202E-4
4	0.0	0.0	0.0	0.0	1.1412092367193202E-4
5	0.0	0.0	0.0	0.0	1.1412092367193202E-4
6	0.0	0.0	0.0	0.0	2.8530230917983004E-4
7	0.0	0.0	0.0	5.706046183596601E-5	2.8530230917983004E-4
8	0.0	0.0	0.0	5.706046183596601E-5	2.8530230917983004E-4
9	0.0	0.0	0.0	5.706046183596601E-5	2.8530230917983004E-4
10	0.0	0.0	0.0	5.706046183596601E-5	2.8530230917983004E-4
11	0.0	0.0	0.0	5.706046183596601E-5	2.8530230917983004E-4
12	0.0	0.0	0.0	5.706046183596601E-5	4.5648369468772806E-4
13	0.0	0.0	0.0	5.706046183596601E-5	4.5648369468772806E-4
14	0.0	0.0	0.0	5.706046183596601E-5	4.5648369468772806E-4
15	0.0	0.0	0.0	1.7118138550789802E-4	4.5648369468772806E-4
16	0.0	0.0	0.0	1.7118138550789802E-4	4.5648369468772806E-4
17	0.0	0.0	0.0	1.7118138550789802E-4	4.5648369468772806E-4
18	0.0	0.0	0.0	1.7118138550789802E-4	4.5648369468772806E-4
19	0.0	0.0	0.0	1.7118138550789802E-4	4.5648369468772806E-4
20	0.0	0.0	0.0	3.9942323285176206E-4	5.706046183596601E-4
21	0.0	5.706046183596601E-5	0.0	3.9942323285176206E-4	6.276650801956261E-4
22	0.0	5.706046183596601E-5	0.0	5.706046183596601E-4	6.847255420315921E-4
23	0.0	5.706046183596601E-5	0.0	6.276650801956261E-4	7.417860038675581E-4
24	0.0	5.706046183596601E-5	0.0	0.0011412092367193202	7.417860038675581E-4
25	0.0	5.706046183596601E-5	0.0	0.0012553301603912522	7.417860038675581E-4
26	0.0	5.706046183596601E-5	0.0	0.0017688743169149462	7.417860038675581E-4
27	0.0	5.706046183596601E-5	0.0	0.0020541766260947763	7.417860038675581E-4
28	0.0	5.706046183596601E-5	0.0	0.0038230509430097225	7.417860038675581E-4
29	0.0	5.706046183596601E-5	0.0	0.007132557729495751	7.417860038675581E-4
30	0.0	5.706046183596601E-5	0.0	0.013466268993287978	7.417860038675581E-4
31	0.0	5.706046183596601E-5	0.0	0.029785561078374257	7.417860038675581E-4
32	0.0	5.706046183596601E-5	0.0	0.047645485633031615	8.559069275394901E-4
33	0.0	5.706046183596601E-5	0.0	0.06807313097030744	8.559069275394901E-4
34	0.0	5.706046183596601E-5	0.0	0.08998434831531839	0.0010270883130473881
35	0.0	5.706046183596601E-5	0.0	0.11526213290865134	0.0010270883130473881
36	0.0	5.706046183596601E-5	0.0	0.15246555402570117	0.0010270883130473881
37	0.0	5.706046183596601E-5	0.0	0.20752889969740837	0.0010270883130473881
38	0.0	5.706046183596601E-5	0.0	0.28347637440107915	0.0010270883130473881
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2300	0.0	25.441895	1
GGTATCA	1040	0.0	20.306427	1
TAGGACG	1005	0.0	19.482485	4
GTATTAC	185	1.8189894E-12	19.025843	1
GACGTGA	600	0.0	18.333239	7
GTCTATA	145	6.31735E-9	18.205763	1
GTATAAT	135	5.5337296E-8	17.92481	1
TTAGGAC	700	0.0	17.913172	3
ACCGTCG	430	0.0	17.906883	8
AACGCAG	3280	0.0	17.774298	6
TACCGTC	450	0.0	17.59991	7
AGGACGT	1125	0.0	17.599907	5
TCAACGC	3325	0.0	17.467579	4
GGACGTG	1145	0.0	17.292486	6
GTCCTAC	990	0.0	17.110046	1
CAACGCA	3425	0.0	16.957577	5
ATACCGT	445	0.0	16.8089	6
TATCAAC	3470	0.0	16.736233	2
GTCCTAA	580	0.0	16.688616	1
ATCAACG	3505	0.0	16.569582	3
>>END_MODULE