FastQCFastQC Report
Thu 2 Feb 2017
SRR4063939_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063939_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1109518
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT27750.2501086057188797No Hit
TATCAACGCAGAGTACTTTTTTTTT21220.19125422030106767No Hit
GTACTTTTTTTTTTTTTTTTTTTTT16870.15204800643162167No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGCCGG400.005271773314.2524718
GCTCGTA602.5649382E-514.2511849
TATACCC500.001500174413.2987065
CTCGTAG655.447793E-513.154345510
GGTATAT655.461646E-513.1507861
TATACTG905.406655E-712.6654345
CGTTATT604.1062213E-412.6608652
CGAGCCG1700.012.29624915
CGAACGA701.0885933E-412.21640316
ATAACGA701.0899698E-412.21474912
ATCCCGT550.003066618212.09136910
GTGTTAG802.8714532E-511.8722371
ACGGTAT1701.8189894E-1211.736279
CCGATAA658.014645E-411.6932799
CCGTGTA658.014645E-411.6932799
TCGCGTA658.014645E-411.6932799
TAACGAA658.017679E-411.69275213
CGAGATT658.026788E-411.691174
GCGTTAT658.0359064E-411.6895881
CGACCCG907.4631334E-611.6099815
Produced by FastQC (version 0.11.5)