##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063939_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1109518
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.241549934295794	32.0	32.0	32.0	32.0	32.0
2	30.826909522873898	32.0	32.0	32.0	32.0	32.0
3	30.86455920498811	32.0	32.0	32.0	32.0	32.0
4	30.890731831299718	32.0	32.0	32.0	32.0	32.0
5	30.797688726095476	32.0	32.0	32.0	32.0	32.0
6	34.454512680280985	36.0	36.0	36.0	32.0	36.0
7	34.38526909883391	36.0	36.0	36.0	32.0	36.0
8	34.34209449508705	36.0	36.0	36.0	32.0	36.0
9	34.48378485071896	36.0	36.0	36.0	32.0	36.0
10	34.17640543010569	36.0	36.0	36.0	32.0	36.0
11	34.481625354433184	36.0	36.0	36.0	32.0	36.0
12	34.27807480365348	36.0	36.0	36.0	32.0	36.0
13	34.36330190226747	36.0	36.0	36.0	32.0	36.0
14	34.27176485645118	36.0	36.0	36.0	32.0	36.0
15	34.20916470034736	36.0	36.0	36.0	32.0	36.0
16	34.20549373692	36.0	36.0	36.0	32.0	36.0
17	34.13972283460025	36.0	36.0	36.0	32.0	36.0
18	34.13555796300736	36.0	36.0	36.0	32.0	36.0
19	34.152639254162615	36.0	36.0	36.0	32.0	36.0
20	34.12665229405922	36.0	36.0	36.0	32.0	36.0
21	34.10166036062506	36.0	36.0	36.0	32.0	36.0
22	34.09353791466204	36.0	36.0	36.0	32.0	36.0
23	34.02692520535945	36.0	36.0	36.0	32.0	36.0
24	34.02419068460358	36.0	36.0	36.0	32.0	36.0
25	33.615538459042575	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	4.0
4	9.0
5	18.0
6	44.0
7	21.0
8	64.0
9	85.0
10	100.0
11	28.0
12	55.0
13	47.0
14	104.0
15	214.0
16	327.0
17	459.0
18	583.0
19	786.0
20	1215.0
21	1703.0
22	2602.0
23	3965.0
24	5995.0
25	8716.0
26	12238.0
27	16242.0
28	22243.0
29	29804.0
30	39332.0
31	53783.0
32	75558.0
33	106776.0
34	229640.0
35	496757.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.93636899706244	17.010194016312592	11.020375409572962	26.033061577052013
2	16.762178941751632	19.913453079707534	37.50153228248799	25.82283569605284
3	18.81955303989653	23.973068828611215	28.6623193434851	28.545058788007154
4	12.418192847208891	15.376454862641499	36.273027907945156	35.93232438220446
5	14.31686334743089	36.99662523931596	33.80412326529729	14.882388147955854
6	33.772656101406305	35.7784089731097	17.139086625700067	13.30984829978393
7	29.968157919410327	30.65386252010415	21.026862022458946	18.351117538026585
8	28.02508317479781	32.88573721272011	19.69786585640739	19.39131375607469
9	27.6671854626512	13.838416992943053	18.389093381473753	40.105304162932
10	15.440035928662127	26.903930509061524	32.263740188156525	25.392293374119816
11	37.019796123018764	21.008581446184703	22.79241485286373	19.179207577932804
12	24.404321711368844	23.742988041341246	29.41929258130265	22.433397665987265
13	29.64440387955836	19.559139455224	25.361341142474743	25.43511552274289
14	23.42161963745706	19.567019216633792	25.71774898334113	31.293612162568014
15	25.088086835989394	27.23346461060072	22.580531704497186	25.0979168489127
16	25.428603639151316	25.749137561723522	24.174065973709723	24.648192825415435
17	23.956858409074922	25.66192346056237	25.557938482017722	24.82327964834499
18	24.428206638458462	25.094145099358595	26.42340625312877	24.054242009054168
19	25.342902524314574	24.87658936571489	25.762854954960645	24.017653155009892
20	25.62747485680783	24.326793848374148	25.345690704911377	24.700040589906642
21	26.48703838830627	24.265962657065003	24.717754264690896	24.52924468993783
22	25.65809412477811	24.322332626170788	25.30676783420646	24.71280541484464
23	24.395486636978077	24.276329379164526	25.53762834661863	25.79055563723876
24	24.542127846113466	25.04119834647381	25.490569254262574	24.926104553150154
25	24.74331139351944	24.526468940746266	25.576134341295948	25.15408532443834
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	224.0
1	224.0
2	388.0
3	552.0
4	552.0
5	552.0
6	1436.5
7	2321.0
8	2321.0
9	2321.0
10	2352.5
11	2384.0
12	2384.0
13	2384.0
14	2673.5
15	2963.0
16	2963.0
17	2963.0
18	5188.5
19	7414.0
20	7414.0
21	7414.0
22	11801.5
23	16189.0
24	16189.0
25	16189.0
26	24029.5
27	31870.0
28	31870.0
29	31870.0
30	40020.0
31	48170.0
32	48170.0
33	48170.0
34	59308.0
35	70446.0
36	70446.0
37	70446.0
38	81517.0
39	92588.0
40	92588.0
41	92588.0
42	105507.0
43	118426.0
44	118426.0
45	118426.0
46	131728.0
47	145030.0
48	145030.0
49	145030.0
50	149476.5
51	153923.0
52	153923.0
53	153923.0
54	142028.5
55	130134.0
56	130134.0
57	130134.0
58	118043.0
59	105952.0
60	105952.0
61	105952.0
62	92338.5
63	78725.0
64	78725.0
65	78725.0
66	64431.5
67	50138.0
68	50138.0
69	50138.0
70	37817.0
71	25496.0
72	25496.0
73	25496.0
74	19405.5
75	13315.0
76	13315.0
77	13315.0
78	10424.5
79	7534.0
80	7534.0
81	7534.0
82	5169.0
83	2804.0
84	2804.0
85	2804.0
86	2161.5
87	1519.0
88	1519.0
89	1519.0
90	1057.0
91	595.0
92	595.0
93	595.0
94	418.5
95	242.0
96	242.0
97	242.0
98	403.0
99	564.0
100	564.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03929634309673209
2	0.0055880120917371325
3	0.002072972227579904
4	0.004416332137018057
5	0.009553698092324776
6	0.014330547138487163
7	0.026497992822108338
8	0.03677272473272178
9	0.048669782734484704
10	0.05894451464509814
11	0.05533934555365483
12	0.06291020064568578
13	0.0659745943734126
14	0.06660549896441519
15	0.06029645305438939
16	0.0669660158735595
17	0.06345097600940228
18	0.07724074778417295
19	0.07399609560187397
20	0.07823216928431986
21	0.07399609560187397
22	0.07769139392060337
23	0.08102617533018842
24	0.07724074778417295
25	0.07922359078446677
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1109518.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.84232478114721
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.89524475998533	43.49851556794056
2	13.209508503487916	14.752993281021961
3	3.9275355007870947	6.5796813907331595
4	1.637219154568906	3.6570449506940848
5	0.8314452470355301	2.321491776134963
6	0.5231967465940897	1.752991358864804
7	0.33999787443960366	1.329039021054924
8	0.23883052086779585	1.066948121116001
9	0.19799460269111432	0.9950831017552269
>10	1.045261264091667	10.578554918711928
>50	0.08104058511680019	3.134034219915064
>100	0.0660695835500569	7.298278518225773
>500	0.005681658230326304	2.1479653183890446
>1k	9.739985537702236E-4	0.8873784554425135
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2775	0.2501086057188797	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2122	0.19125422030106767	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1687	0.15204800643162167	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.8025845457216556E-4
2	0.0	0.0	0.0	0.0	1.8025845457216556E-4
3	0.0	0.0	0.0	9.012922728608278E-5	1.8025845457216556E-4
4	0.0	0.0	0.0	2.703876818582484E-4	1.8025845457216556E-4
5	0.0	0.0	0.0	2.703876818582484E-4	1.8025845457216556E-4
6	0.0	0.0	0.0	2.703876818582484E-4	1.8025845457216556E-4
7	0.0	0.0	0.0	2.703876818582484E-4	1.8025845457216556E-4
8	0.0	0.0	0.0	2.703876818582484E-4	1.8025845457216556E-4
9	0.0	0.0	0.0	2.703876818582484E-4	1.8025845457216556E-4
10	0.0	0.0	0.0	3.605169091443311E-4	1.8025845457216556E-4
11	0.0	0.0	0.0	4.506461364304139E-4	1.8025845457216556E-4
12	0.0	0.0	0.0	4.506461364304139E-4	3.605169091443311E-4
13	0.0	0.0	0.0	5.407753637164968E-4	3.605169091443311E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGCCGG	40	0.0052717733	14.25247	18
GCTCGTA	60	2.5649382E-5	14.251184	9
TATACCC	50	0.0015001744	13.298706	5
CTCGTAG	65	5.447793E-5	13.1543455	10
GGTATAT	65	5.461646E-5	13.150786	1
TATACTG	90	5.406655E-7	12.665434	5
CGTTATT	60	4.1062213E-4	12.660865	2
CGAGCCG	170	0.0	12.296249	15
CGAACGA	70	1.0885933E-4	12.216403	16
ATAACGA	70	1.0899698E-4	12.214749	12
ATCCCGT	55	0.0030666182	12.091369	10
GTGTTAG	80	2.8714532E-5	11.872237	1
ACGGTAT	170	1.8189894E-12	11.73627	9
CCGATAA	65	8.014645E-4	11.693279	9
CCGTGTA	65	8.014645E-4	11.693279	9
TCGCGTA	65	8.014645E-4	11.693279	9
TAACGAA	65	8.017679E-4	11.692752	13
CGAGATT	65	8.026788E-4	11.69117	4
GCGTTAT	65	8.0359064E-4	11.689588	1
CGACCCG	90	7.4631334E-6	11.609981	5
>>END_MODULE