##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063939_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1109518
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.295505796210605	32.0	32.0	32.0	32.0	32.0
2	31.39360244718878	32.0	32.0	32.0	32.0	32.0
3	31.476807947234747	32.0	32.0	32.0	32.0	32.0
4	31.559867437932507	32.0	32.0	32.0	32.0	32.0
5	31.505777283469037	32.0	32.0	32.0	32.0	32.0
6	35.09181013737497	36.0	36.0	36.0	36.0	36.0
7	35.11334651623498	36.0	36.0	36.0	36.0	36.0
8	35.05829918937773	36.0	36.0	36.0	36.0	36.0
9	35.177082300602606	36.0	36.0	36.0	36.0	36.0
10	35.02146247289363	36.0	36.0	36.0	36.0	36.0
11	35.17928325633293	36.0	36.0	36.0	36.0	36.0
12	35.080334884156905	36.0	36.0	36.0	36.0	36.0
13	35.11731039965102	36.0	36.0	36.0	36.0	36.0
14	35.07056397462682	36.0	36.0	36.0	36.0	36.0
15	35.03493318720381	36.0	36.0	36.0	36.0	36.0
16	35.05000459659059	36.0	36.0	36.0	36.0	36.0
17	35.0090165278977	36.0	36.0	36.0	36.0	36.0
18	35.00765557656568	36.0	36.0	36.0	36.0	36.0
19	35.013278739056055	36.0	36.0	36.0	36.0	36.0
20	35.010586579037025	36.0	36.0	36.0	36.0	36.0
21	34.99626234094445	36.0	36.0	36.0	36.0	36.0
22	34.9856631438156	36.0	36.0	36.0	36.0	36.0
23	34.93437150185937	36.0	36.0	36.0	36.0	36.0
24	34.90394928248122	36.0	36.0	36.0	32.0	36.0
25	34.88371887612459	36.0	36.0	36.0	32.0	36.0
26	34.82045536890794	36.0	36.0	36.0	32.0	36.0
27	34.81892497462862	36.0	36.0	36.0	32.0	36.0
28	34.79545171867424	36.0	36.0	36.0	32.0	36.0
29	34.770188496265945	36.0	36.0	36.0	32.0	36.0
30	34.75290621693384	36.0	36.0	36.0	32.0	36.0
31	34.74172748887354	36.0	36.0	36.0	32.0	36.0
32	34.69471157745976	36.0	36.0	36.0	32.0	36.0
33	34.684407102904146	36.0	36.0	36.0	32.0	36.0
34	34.66436596792481	36.0	36.0	36.0	32.0	36.0
35	34.62102462510748	36.0	36.0	36.0	32.0	36.0
36	34.59662934715796	36.0	36.0	36.0	32.0	36.0
37	34.58186888360531	36.0	36.0	36.0	32.0	36.0
38	34.54129811323475	36.0	36.0	36.0	32.0	36.0
39	34.54490238103393	36.0	36.0	36.0	32.0	36.0
40	34.53124690180781	36.0	36.0	36.0	32.0	36.0
41	34.50244700852082	36.0	36.0	36.0	32.0	36.0
42	34.436811300042	36.0	36.0	36.0	32.0	36.0
43	34.43221470945041	36.0	36.0	36.0	32.0	36.0
44	34.366706083182066	36.0	36.0	36.0	32.0	36.0
45	34.32553955861915	36.0	36.0	36.0	32.0	36.0
46	34.30146784459558	36.0	36.0	36.0	32.0	36.0
47	34.263811853435456	36.0	36.0	36.0	32.0	36.0
48	34.22574487299891	36.0	36.0	36.0	32.0	36.0
49	34.209123240902805	36.0	36.0	36.0	32.0	36.0
50	33.604971708435556	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	3.0
18	2.0
19	2.0
20	6.0
21	26.0
22	104.0
23	322.0
24	926.0
25	2076.0
26	4184.0
27	7689.0
28	12639.0
29	19334.0
30	27748.0
31	39124.0
32	56971.0
33	92104.0
34	192128.0
35	654130.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.047523545896134	17.867569713789706	11.51204808960285	26.57285865071131
2	15.982403106224888	20.56260669085744	37.18078365843063	26.274206544487043
3	18.128281276053052	24.534774203142813	28.980770877365337	28.356173643438797
4	12.142840404572075	15.944130694589903	36.3224391132005	35.59058978763752
5	13.943982882657155	37.36703685744621	33.62811599271035	15.060864267186291
6	33.80272677209993	36.196921723596844	16.859318063625885	13.14103344067734
7	29.938162281425164	30.734454722189923	20.968493497621036	18.35888949876388
8	28.062365820112877	33.040653689259656	19.490445400615403	19.40653509001206
9	27.7423617113516	13.615752553607047	18.371820327730553	40.2700654073108
10	15.378604409698344	27.088739253129084	32.21659310559896	25.316063231573604
11	37.271049230386524	20.971809380289457	22.636406079036124	19.12073531028789
12	24.42161769490688	23.787017233625082	29.31194384583562	22.479421225632414
13	29.7653919297708	19.51474074140837	25.316761453254138	25.403105875566688
14	23.49091584243191	19.389210050526625	25.76272133564786	31.357152771393604
15	25.151085517332135	27.306346898657107	22.457189056766694	25.085378527244067
16	25.453331158197006	25.769146532385044	24.02504170763824	24.752480601779705
17	23.949859308276206	25.757581219953167	25.593906543201644	24.698652928568983
18	24.581190880700127	25.029314610437893	26.445248599613347	23.944245909248636
19	25.340463156073177	24.800679213856828	25.794714461595035	24.06414316847496
20	25.748931514158453	24.27477255807498	25.35632718469528	24.61996874307129
21	26.568834394755196	24.086134699932764	24.913701264873573	24.43132964043846
22	25.61562768697759	24.28847481519002	25.364978305894994	24.7309191919374
23	24.384750371560752	24.13752389570522	25.632779905345515	25.84494582738851
24	24.612646066073914	24.857798227153303	25.663555697759833	24.866000009012946
25	24.76964662173916	24.500964856857784	25.641668116280346	25.08772040512271
26	24.630068114156334	25.12769714302711	25.84642452506217	24.395810217754388
27	25.13864865668763	24.773605874574905	25.235452594462704	24.85229287427476
28	24.471708640048025	24.700285631105938	25.988554926528177	24.83945080231786
29	24.50754445056337	24.881770108995926	25.745138987157173	24.86554645328353
30	24.60751813014769	24.994276587728645	25.839538954355113	24.558666327768556
31	25.02034823193469	24.767606516121297	25.116974166083033	25.095071085860976
32	24.7654302293511	24.98174817346536	25.01527745480307	25.237544142380468
33	24.638882930109222	24.540998707495625	25.48351741455861	25.33660094783654
34	24.980148518822627	24.808447853020624	25.620263021985796	24.59114060617095
35	25.568801989398647	24.557518335149496	25.50111268241637	24.372566993035484
36	24.687768155236192	25.23381333871747	25.285551525465284	24.792866980581056
37	25.415804772527544	24.893575790351292	25.12999391604137	24.56062552107979
38	24.757308681724194	24.835005957992994	25.427109679350014	24.9805756809328
39	25.076027566414833	24.821310432225953	25.021496828203837	25.081165173155373
40	25.376139595040986	24.98100487158572	25.453201681846245	24.189653851527044
41	24.582213060748444	25.223325945468	25.654339837599156	24.540121156184398
42	25.466698392783748	25.337450518073073	25.234070244507418	23.961780844635765
43	24.810274898602973	24.572960793150067	25.581433077963045	25.035331230283912
44	24.589728059644457	25.18701983221392	25.071923771617687	25.15132833652394
45	24.741191124698737	25.36579342518202	25.09792646457072	24.795088985548524
46	24.595518550130823	25.00299682651844	25.07483053284026	25.32665409051047
47	24.692608729353275	25.028526414648788	25.46466960733593	24.814195248662006
48	25.25777470130582	25.27372789086216	24.857052211094047	24.61144519673798
49	24.70031869867113	25.314372444822585	24.975304458176208	25.010004398330075
50	24.71052145345405	25.637429641413434	24.794613765722243	24.85743513941027
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	72.0
1	58.5
2	45.0
3	136.0
4	227.0
5	254.0
6	281.0
7	267.0
8	253.0
9	324.5
10	396.0
11	591.5
12	787.0
13	1404.5
14	2022.0
15	2608.5
16	3195.0
17	3403.5
18	3612.0
19	3594.0
20	3576.0
21	3947.5
22	4319.0
23	4909.5
24	5500.0
25	6244.5
26	6989.0
27	8413.5
28	9838.0
29	11677.5
30	13517.0
31	15871.5
32	18226.0
33	21098.5
34	23971.0
35	27329.5
36	30688.0
37	35576.0
38	40464.0
39	44746.0
40	49028.0
41	53529.0
42	58030.0
43	59990.5
44	61951.0
45	68036.0
46	74121.0
47	77822.5
48	81524.0
49	84240.5
50	86957.0
51	85551.0
52	84145.0
53	82824.5
54	81504.0
55	80552.0
56	79600.0
57	76186.5
58	72773.0
59	66400.0
60	60027.0
61	52350.5
62	44674.0
63	38946.5
64	33219.0
65	28151.5
66	23084.0
67	20058.0
68	17032.0
69	15252.0
70	13472.0
71	10550.5
72	7629.0
73	6282.0
74	4935.0
75	3858.0
76	2781.0
77	2452.5
78	2124.0
79	1699.0
80	1274.0
81	980.0
82	686.0
83	597.5
84	509.0
85	367.0
86	225.0
87	155.0
88	85.0
89	70.0
90	55.0
91	47.0
92	39.0
93	29.0
94	19.0
95	20.0
96	21.0
97	14.0
98	7.0
99	8.5
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008021501228461368
2	3.605169091443311E-4
3	2.7038768185824833E-4
4	0.0
5	0.0
6	9.012922728608278E-5
7	9.012922728608278E-5
8	0.0
9	8.11163045574745E-4
10	8.11163045574745E-4
11	0.0
12	9.012922728608278E-5
13	7.210338182886622E-4
14	3.605169091443311E-4
15	0.004236073682445891
16	8.11163045574745E-4
17	0.0
18	2.7038768185824833E-4
19	0.0
20	3.605169091443311E-4
21	0.0
22	0.0
23	8.11163045574745E-4
24	2.7038768185824833E-4
25	0.0024334891367242353
26	0.005137365955306719
27	0.005317624409878884
28	0.004055815227873726
29	0.0024334891367242353
30	0.0036051690914433113
31	0.007660984319317036
32	0.0036051690914433113
33	0.003965686000587642
34	0.0026137475912964008
35	0.003334781409585063
36	0.00775111354660312
37	0.00387555677330156
38	0.007570855092030954
39	0.004686719818876304
40	0.002072972227579904
41	0.0036952983187293945
42	0.001982843000293821
43	0.00162232609114949
44	9.012922728608278E-4
45	0.0010815507274329933
46	9.914215001469106E-4
47	0.0018025845457216557
48	0.001982843000293821
49	5.407753637164967E-4
50	0.002072972227579904
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1109518.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.10192464600358
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.7196744490406	46.524842674451314
2	13.010054749899547	15.378385509378878
3	3.7092146586612396	6.576651757561454
4	1.5670494937674144	3.704625643887991
5	0.7629254470254508	2.2545181140308395
6	0.47414739953093	1.6813814326905343
7	0.3234427859931946	1.3381263815544275
8	0.2308734966480027	1.0916054401319693
9	0.1656541595476344	0.8811431689393209
>10	0.913953119817287	9.76903380932436
>50	0.06528535316105955	2.7373012821492013
>100	0.05265860923675473	6.064391670647414
>500	0.005066277670901451	1.9979931152522679
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.8025845457216556E-4
2	0.0	0.0	0.0	0.0	1.8025845457216556E-4
3	0.0	0.0	0.0	0.0	1.8025845457216556E-4
4	0.0	0.0	0.0	1.8025845457216556E-4	1.8025845457216556E-4
5	0.0	0.0	0.0	1.8025845457216556E-4	1.8025845457216556E-4
6	0.0	0.0	0.0	1.8025845457216556E-4	4.506461364304139E-4
7	0.0	0.0	0.0	1.8025845457216556E-4	4.506461364304139E-4
8	0.0	0.0	0.0	1.8025845457216556E-4	4.506461364304139E-4
9	0.0	0.0	0.0	1.8025845457216556E-4	4.506461364304139E-4
10	0.0	0.0	0.0	2.703876818582484E-4	4.506461364304139E-4
11	0.0	0.0	0.0	3.605169091443311E-4	4.506461364304139E-4
12	0.0	0.0	0.0	3.605169091443311E-4	9.012922728608278E-4
13	0.0	0.0	0.0	3.605169091443311E-4	9.012922728608278E-4
14	0.0	0.0	0.0	3.605169091443311E-4	9.012922728608278E-4
15	0.0	0.0	0.0	3.605169091443311E-4	0.0011716799547190763
16	0.0	0.0	0.0	3.605169091443311E-4	0.0011716799547190763
17	0.0	0.0	0.0	3.605169091443311E-4	0.0011716799547190763
18	0.0	0.0	0.0	3.605169091443311E-4	0.001261809182005159
19	0.0	0.0	0.0	3.605169091443311E-4	0.001261809182005159
20	0.0	0.0	0.0	3.605169091443311E-4	0.0015321968638634074
21	0.0	0.0	0.0	3.605169091443311E-4	0.001712455318435573
22	0.0	0.0	0.0	7.210338182886622E-4	0.001712455318435573
23	0.0	0.0	0.0	0.0014420676365773245	0.001712455318435573
24	0.0	0.0	0.0	0.0024334891367242353	0.001712455318435573
25	0.0	0.0	0.0	0.0026137475912964008	0.001712455318435573
26	0.0	0.0	0.0	0.003064393727726815	0.001982843000293821
27	0.0	0.0	0.0	0.003965686000587642	0.002163101454865987
28	0.0	0.0	0.0	0.006309045910025795	0.002163101454865987
29	0.0	0.0	0.0	0.012077316456335094	0.002163101454865987
30	0.0	0.0	0.0	0.021450756094087704	0.002163101454865987
31	0.0	0.0	0.0	0.04209034914260066	0.002163101454865987
32	0.0	0.0	0.0	0.0670561451008456	0.002163101454865987
33	0.0	0.0	0.0	0.08967858114965237	0.00225323068215207
34	0.0	0.0	0.0	0.11473450633518338	0.00225323068215207
35	0.0	0.0	0.0	0.14537844361245153	0.00225323068215207
36	0.0	0.0	0.0	0.1843142698000393	0.00225323068215207
37	0.0	0.0	0.0	0.24749485812758332	0.00225323068215207
38	0.0	0.0	0.0	0.337443826959094	0.0023433599094381525
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGCATA	50	0.0025792161	22.001598	37
GTAATAC	165	0.0	21.332	3
TAGTACG	75	5.4747466E-5	20.532051	4
CGTGCGG	65	4.93389E-4	20.306423	10
GTATCAA	2160	0.0	19.658836	1
AATCACG	135	2.5956979E-9	19.556978	34
GTATAGG	120	3.158966E-7	18.334667	1
GTCCTAA	195	1.8189894E-12	18.052595	1
ACCGTCG	275	0.0	17.5989	8
GTATATG	165	1.6570993E-9	17.334595	1
ATACCGT	270	0.0	17.110043	6
GTAGCAC	155	1.4397301E-8	17.031195	3
GTATTAG	300	0.0	16.867893	1
TATGGTA	105	3.7759153E-5	16.760857	2
CGTGATT	80	0.001987245	16.5012	28
CGTCGTA	280	0.0	16.49897	10
TACGAGC	215	1.2732926E-11	16.371069	14
AACGCAG	2620	0.0	16.28906	6
GTATTGG	205	1.05501385E-10	16.098732	1
GTCTTAG	330	0.0	16.001163	1
>>END_MODULE