FastQCFastQC Report
Thu 2 Feb 2017
SRR4063938_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063938_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2492
Sequences flagged as poor quality0
Sequence length25
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGG2068.26645264847512No Hit
GACATGAGAAGGTCACACTGACATG200.8025682182985553No Hit
TCTCATGTCAGTGTGACCTTCTCAT180.7223113964686998No Hit
CTCCTAGCATGGACAGGCCAGCTTG150.6019261637239166No Hit
GTGTAGCAATGGGGGATGGGGAACT120.4815409309791332No Hit
GACATGAGAAGGTCAAACTGACATG110.44141252006420545No Hit
CCTGTAGGGTTCTCAGAGGCTCTTG90.3611556982343499No Hit
TCTCAGGACAGTATGACCCACTGGG90.3611556982343499No Hit
GTCCTGAGAAGGTCACACTGACATG90.3611556982343499No Hit
GGGTCATACTGTCCTGAGAAGGTCA90.3611556982343499No Hit
CCTCTAAGGTCCCTCCCTTTGAGAC80.32102728731942215No Hit
GTATCAACGCAGAGTACATGGGATG80.32102728731942215No Hit
GTCTCGGGATGGCCTTTCCTTCAGT70.2808988764044944No Hit
GTCTTATTACTAGAGGTGGACTCTA70.2808988764044944No Hit
CCATAGAGCATCCTCCCCTCTGCTG70.2808988764044944No Hit
GGGATAATTATGGGTTAGAGTTTTT60.2407704654895666No Hit
TCTCATGTCAGTGTGACCTTCTCAG60.2407704654895666No Hit
AGCATGGACAGGCCAGCTTGGATGT60.2407704654895666No Hit
GCCCAGCACTTGTCCTGCATTCTCC60.2407704654895666No Hit
CTCTATTTCTTTTAGACAGGAGCAA60.2407704654895666No Hit
CCTTTCCCTCAGTCTCTTCTCCACT60.2407704654895666No Hit
GTGCTGGGCAAAGGGCCTGAAGCCC50.20064205457463882No Hit
CGCCTAACCTCTGGATATGGTCCCC50.20064205457463882No Hit
TGTGTACTCTGTGGATAGCATTTTC50.20064205457463882No Hit
GTACATGGGATGGGTGGGCTAGGTG50.20064205457463882No Hit
GAGATGTACAGAGGATCAGGAAATT50.20064205457463882No Hit
TATCAACGCAGAGTACATGGGGTGA50.20064205457463882No Hit
GGGCATGTTGTCCTGAGGGTCACAT50.20064205457463882No Hit
GGGCAAAGGGCCTGAAGCCCCTGGG50.20064205457463882No Hit
CCTCAGGACAACATGCCCTGTGGGA50.20064205457463882No Hit
CTTCTGGGGAGAATGCAGGACAAGT50.20064205457463882No Hit
GTATCAACGCAGAGTACATGGGACC50.20064205457463882No Hit
GTGTGTAGCAATGGGGGATGGGGAA50.20064205457463882No Hit
CTTGTAGGCTATTTCAGCTAATGTC50.20064205457463882No Hit
GTACATGGGACCTCCTTTCCCTCAG40.16051364365971107No Hit
GACATGAGAAGGTCAAACTGCCCCA40.16051364365971107No Hit
GTCCTGAGGGTCACATTGCCCCAGT40.16051364365971107No Hit
TAATTATCCCTGTCTAAAAGACCTG40.16051364365971107No Hit
GGCCTGAAGCCCCTGGGGCAGTTTG40.16051364365971107No Hit
GCCCCAGGGGCTTCAGGCCCTTTGC40.16051364365971107No Hit
GTGGTGGACCATAGAGCATCCTCCC40.16051364365971107No Hit
CTTTTAGACAGGAGCAATTCTGAAT40.16051364365971107No Hit
TGTCAGTGTGACCTTCTCATGTCAG40.16051364365971107No Hit
CACCCAACCATGTCCAAAATTGTAA40.16051364365971107No Hit
ACACTGACATGAGAAGGTCAAACTG40.16051364365971107No Hit
GGAATGGAATTGCCATCCCATAGTA40.16051364365971107No Hit
TATCAACGCAGAGTACATGGGATGG40.16051364365971107No Hit
CCCCTCTGCTGACTCCTTCTGGGGA40.16051364365971107No Hit
GGATAATTATGGGTTAGAGTTTTTT40.16051364365971107No Hit
GGGGATAGCTCCCAGAAACTCCCAG40.16051364365971107No Hit
ACGCAGAGTACATGGGATGGGTGGG40.16051364365971107No Hit
GATGTACAGAGGATCAGGAAATTGA40.16051364365971107No Hit
GGACAGTATGACCCACTGGGGCAAT40.16051364365971107No Hit
CTTCAGGCCCTTTGCCCAGCACTTG40.16051364365971107No Hit
ATCCAGAGGTTAGGCGTGGCCCCCT40.16051364365971107No Hit
GAACAGAGGTGTGTAGCAATGGGGG40.16051364365971107No Hit
GGTCACACTGACATGAGAAGGTCAA40.16051364365971107No Hit
CATTGCCCCAGTGGGTCATACTGTC40.16051364365971107No Hit
CACTTGTCCTGCATTCTCCCCAGAA30.1203852327447833No Hit
GTATCAACGCAGAGTACATGGGGTG30.1203852327447833No Hit
GTACACAGGAGACATCCAAGCTGGC30.1203852327447833No Hit
GTACATGGGGGATCCCCCATGTGCC30.1203852327447833No Hit
GTATCAACGCAGAGTACATGGGGCC30.1203852327447833No Hit
GGGCTAGGTGGTGGACCATAGAGCA30.1203852327447833No Hit
GGACAGGCCAGCTTGGATGTCTCCT30.1203852327447833No Hit
GTATCAACGCAGAGTACATGGGCCC30.1203852327447833No Hit
AGTTTGGTGTGGCCGGGACCTAGTG30.1203852327447833No Hit
GGGTTCTCAGAGGCTCTTGTTTTCC30.1203852327447833No Hit
CCTCTGGAGGTAAGAGGTAGAGGGG30.1203852327447833No Hit
GGACAACATGCCCTGTGGGAAAATG30.1203852327447833No Hit
CCCCTGAGGGCCAGCAGAAAAAAAT30.1203852327447833No Hit
CAGTGTGACCTTCTCATGTCAGTGT30.1203852327447833No Hit
GTATCAACGCAGAGTACATGGGGAA30.1203852327447833No Hit
GAACTGGGGATAGCTCCCAGAAACT30.1203852327447833No Hit
CTGGTGAGAAATCCCGAGTCACTCT30.1203852327447833No Hit
GGAGTAAACCCCTGAGGGCCAGCAG30.1203852327447833No Hit
GAGAGAGTCTGGCTAGCTCAGGATG30.1203852327447833No Hit
CCAGTGGGTCATACTGTCCTGAGAA30.1203852327447833No Hit
ATGCCCAACAGTGGGGAAAGGGAAC30.1203852327447833No Hit
TATCAACGCAGAGTACATGGGATCC30.1203852327447833No Hit
ACTCTGTGGATAGCATTTTCCCACA30.1203852327447833No Hit
CTAAGGTCCCTCCCTTTGAGACCTG30.1203852327447833No Hit
CCTTAGAGGAAATGCCCAACAGTGG30.1203852327447833No Hit
GACCTGGGAGGTGAGAGATGCTCAG30.1203852327447833No Hit
TATCAACGCAGAGTACATGGGGAAG30.1203852327447833No Hit
GCTTGGATGTCTCCTGTGTACTCTG30.1203852327447833No Hit
CATCTAACCTCCTGCTGGAAGGCCT30.1203852327447833No Hit
ATTTCAGCTAATGTCATACCTGTAG30.1203852327447833No Hit
TCACATGGCCATGGCCGACCTCTCT30.1203852327447833No Hit
GGTATCAACGCAGAGTACATGGGGA30.1203852327447833No Hit
GGAGGGGGGATCAGGAGTAAACCCC30.1203852327447833No Hit
ATCAACGCAGAGTACATGGGATCCC30.1203852327447833No Hit
GTCTAAAAGAAATAGAGGGATGAAG30.1203852327447833No Hit
GATCCCTAGGTGGGTCAGTCTCGGG30.1203852327447833No Hit
GTACATGGGGCCTCTCCTAGCATGG30.1203852327447833No Hit
GTACATGGGGTGAGGAGGAAGAGAT30.1203852327447833No Hit
CTACAGGTATGACATTAGCTGAAAT30.1203852327447833No Hit
GGTTAGGCGTGGCCCCCTGGTTGAA30.1203852327447833No Hit
AAATATCGATATCAGGATATTCAGA30.1203852327447833No Hit
CTCTACAAGTTCCCTTTCCCCACTG30.1203852327447833No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers