##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063938_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2492
Sequences flagged as poor quality	0
Sequence length	25
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.252407704654896	32.0	32.0	32.0	32.0	32.0
2	30.957865168539325	32.0	32.0	32.0	32.0	32.0
3	30.91813804173355	32.0	32.0	32.0	32.0	32.0
4	31.015650080256822	32.0	32.0	32.0	32.0	32.0
5	31.036115569823433	32.0	32.0	32.0	32.0	32.0
6	34.49077046548957	36.0	36.0	36.0	32.0	36.0
7	34.47512038523274	36.0	36.0	36.0	32.0	36.0
8	34.46027287319422	36.0	36.0	36.0	32.0	36.0
9	34.541332263242374	36.0	36.0	36.0	32.0	36.0
10	34.43659711075441	36.0	36.0	36.0	32.0	36.0
11	34.42616372391653	36.0	36.0	36.0	32.0	36.0
12	34.364365971107546	36.0	36.0	36.0	32.0	36.0
13	34.543739967897274	36.0	36.0	36.0	32.0	36.0
14	34.44863563402889	36.0	36.0	36.0	32.0	36.0
15	34.29935794542536	36.0	36.0	36.0	32.0	36.0
16	34.33988764044944	36.0	36.0	36.0	32.0	36.0
17	34.14767255216693	36.0	36.0	36.0	32.0	36.0
18	34.24317817014446	36.0	36.0	36.0	32.0	36.0
19	34.44060995184591	36.0	36.0	36.0	32.0	36.0
20	34.102728731942214	36.0	36.0	36.0	32.0	36.0
21	34.20545746388443	36.0	36.0	36.0	32.0	36.0
22	34.26243980738363	36.0	36.0	36.0	32.0	36.0
23	34.18860353130016	36.0	36.0	36.0	32.0	36.0
24	34.307383627608345	36.0	36.0	36.0	32.0	36.0
25	33.8045746388443	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	1.0
12	0.0
13	0.0
14	1.0
15	0.0
16	2.0
17	2.0
18	1.0
19	0.0
20	7.0
21	3.0
22	4.0
23	8.0
24	9.0
25	16.0
26	24.0
27	38.0
28	48.0
29	67.0
30	86.0
31	88.0
32	156.0
33	228.0
34	443.0
35	1259.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	50.04012841091493	15.208667736757626	10.79454253611557	23.95666131621188
2	25.40128410914928	19.58266452648475	32.142857142857146	22.87319422150883
3	26.364365971107546	21.187800963081862	27.32744783306581	25.120385232744784
4	21.629213483146067	15.890850722311397	31.09951845906902	31.380417335473513
5	20.626003210272874	32.985553772070624	31.621187800963078	14.767255216693421
6	39.24558587479936	31.781701444622794	16.051364365971107	12.921348314606742
7	36.75762439807384	29.735152487961475	16.934189406099517	16.573033707865168
8	35.608189482135685	28.904054596547574	17.824167001204337	17.663588920112407
9	33.89558232931727	15.783132530120481	17.83132530120482	32.48995983935743
10	24.136546184738954	26.385542168674696	29.67871485943775	19.799196787148592
11	41.68674698795181	19.518072289156628	21.244979919678713	17.55020080321285
12	33.21285140562249	21.044176706827308	25.863453815261046	19.879518072289155
13	34.17670682730924	18.755020080321287	24.538152610441767	22.53012048192771
14	31.08433734939759	17.14859437751004	23.6144578313253	28.152610441767067
15	33.13253012048193	25.622489959839356	20.32128514056225	20.923694779116467
16	32.00803212851406	27.148594377510037	19.95983935742972	20.883534136546185
17	30.44176706827309	24.819277108433734	21.847389558232933	22.89156626506024
18	30.76305220883534	23.815261044176708	21.72690763052209	23.694779116465863
19	29.116465863453815	22.48995983935743	25.702811244979916	22.690763052208833
20	33.93574297188755	22.690763052208833	22.73092369477912	20.6425702811245
21	31.606425702811247	23.57429718875502	23.012048192771083	21.80722891566265
22	33.29317269076305	20.160642570281123	23.895582329317268	22.650602409638555
23	30.361445783132528	24.97991967871486	22.89156626506024	21.76706827309237
24	29.799196787148595	23.93574297188755	24.417670682730925	21.847389558232933
25	33.93574297188755	21.566265060240962	24.738955823293175	19.759036144578314
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1.0
1	1.0
2	0.5
3	0.0
4	0.0
5	0.0
6	1.0
7	2.0
8	2.0
9	2.0
10	2.0
11	2.0
12	2.0
13	2.0
14	8.0
15	14.0
16	14.0
17	14.0
18	20.5
19	27.0
20	27.0
21	27.0
22	32.0
23	37.0
24	37.0
25	37.0
26	47.0
27	57.0
28	57.0
29	57.0
30	77.0
31	97.0
32	97.0
33	97.0
34	129.0
35	161.0
36	161.0
37	161.0
38	182.5
39	204.0
40	204.0
41	204.0
42	216.5
43	229.0
44	229.0
45	229.0
46	253.0
47	277.0
48	277.0
49	277.0
50	282.5
51	288.0
52	288.0
53	288.0
54	297.0
55	306.0
56	306.0
57	306.0
58	285.0
59	264.0
60	264.0
61	264.0
62	206.0
63	148.0
64	148.0
65	148.0
66	112.5
67	77.0
68	77.0
69	77.0
70	66.5
71	56.0
72	56.0
73	56.0
74	37.5
75	19.0
76	19.0
77	19.0
78	12.5
79	6.0
80	6.0
81	6.0
82	4.5
83	3.0
84	3.0
85	3.0
86	2.0
87	1.0
88	1.0
89	1.0
90	2.0
91	3.0
92	3.0
93	3.0
94	3.0
95	3.0
96	3.0
97	3.0
98	106.5
99	210.0
100	210.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.04012841091492777
9	0.08025682182985554
10	0.08025682182985554
11	0.08025682182985554
12	0.08025682182985554
13	0.08025682182985554
14	0.08025682182985554
15	0.08025682182985554
16	0.08025682182985554
17	0.08025682182985554
18	0.08025682182985554
19	0.08025682182985554
20	0.08025682182985554
21	0.08025682182985554
22	0.08025682182985554
23	0.08025682182985554
24	0.08025682182985554
25	0.08025682182985554
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2492.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	71.22792937399679
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.53521126760563	61.63723916532905
2	7.830985915492958	11.15569823434992
3	2.3661971830985915	5.056179775280898
4	1.352112676056338	3.8523274478330656
5	0.7323943661971831	2.608346709470305
6	0.3380281690140845	1.4446227929373996
7	0.16901408450704225	0.8426966292134831
8	0.11267605633802817	0.6420545746388443
9	0.22535211267605634	1.4446227929373996
>10	0.28169014084507044	3.0497592295345104
>50	0.0	0.0
>100	0.056338028169014086	8.26645264847512
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGGGGGGGGGGGGGGGGGGGGGGGG	206	8.26645264847512	No Hit
GACATGAGAAGGTCACACTGACATG	20	0.8025682182985553	No Hit
TCTCATGTCAGTGTGACCTTCTCAT	18	0.7223113964686998	No Hit
CTCCTAGCATGGACAGGCCAGCTTG	15	0.6019261637239166	No Hit
GTGTAGCAATGGGGGATGGGGAACT	12	0.4815409309791332	No Hit
GACATGAGAAGGTCAAACTGACATG	11	0.44141252006420545	No Hit
CCTGTAGGGTTCTCAGAGGCTCTTG	9	0.3611556982343499	No Hit
TCTCAGGACAGTATGACCCACTGGG	9	0.3611556982343499	No Hit
GTCCTGAGAAGGTCACACTGACATG	9	0.3611556982343499	No Hit
GGGTCATACTGTCCTGAGAAGGTCA	9	0.3611556982343499	No Hit
CCTCTAAGGTCCCTCCCTTTGAGAC	8	0.32102728731942215	No Hit
GTATCAACGCAGAGTACATGGGATG	8	0.32102728731942215	No Hit
GTCTCGGGATGGCCTTTCCTTCAGT	7	0.2808988764044944	No Hit
GTCTTATTACTAGAGGTGGACTCTA	7	0.2808988764044944	No Hit
CCATAGAGCATCCTCCCCTCTGCTG	7	0.2808988764044944	No Hit
GGGATAATTATGGGTTAGAGTTTTT	6	0.2407704654895666	No Hit
TCTCATGTCAGTGTGACCTTCTCAG	6	0.2407704654895666	No Hit
AGCATGGACAGGCCAGCTTGGATGT	6	0.2407704654895666	No Hit
GCCCAGCACTTGTCCTGCATTCTCC	6	0.2407704654895666	No Hit
CTCTATTTCTTTTAGACAGGAGCAA	6	0.2407704654895666	No Hit
CCTTTCCCTCAGTCTCTTCTCCACT	6	0.2407704654895666	No Hit
GTGCTGGGCAAAGGGCCTGAAGCCC	5	0.20064205457463882	No Hit
CGCCTAACCTCTGGATATGGTCCCC	5	0.20064205457463882	No Hit
TGTGTACTCTGTGGATAGCATTTTC	5	0.20064205457463882	No Hit
GTACATGGGATGGGTGGGCTAGGTG	5	0.20064205457463882	No Hit
GAGATGTACAGAGGATCAGGAAATT	5	0.20064205457463882	No Hit
TATCAACGCAGAGTACATGGGGTGA	5	0.20064205457463882	No Hit
GGGCATGTTGTCCTGAGGGTCACAT	5	0.20064205457463882	No Hit
GGGCAAAGGGCCTGAAGCCCCTGGG	5	0.20064205457463882	No Hit
CCTCAGGACAACATGCCCTGTGGGA	5	0.20064205457463882	No Hit
CTTCTGGGGAGAATGCAGGACAAGT	5	0.20064205457463882	No Hit
GTATCAACGCAGAGTACATGGGACC	5	0.20064205457463882	No Hit
GTGTGTAGCAATGGGGGATGGGGAA	5	0.20064205457463882	No Hit
CTTGTAGGCTATTTCAGCTAATGTC	5	0.20064205457463882	No Hit
GTACATGGGACCTCCTTTCCCTCAG	4	0.16051364365971107	No Hit
GACATGAGAAGGTCAAACTGCCCCA	4	0.16051364365971107	No Hit
GTCCTGAGGGTCACATTGCCCCAGT	4	0.16051364365971107	No Hit
TAATTATCCCTGTCTAAAAGACCTG	4	0.16051364365971107	No Hit
GGCCTGAAGCCCCTGGGGCAGTTTG	4	0.16051364365971107	No Hit
GCCCCAGGGGCTTCAGGCCCTTTGC	4	0.16051364365971107	No Hit
GTGGTGGACCATAGAGCATCCTCCC	4	0.16051364365971107	No Hit
CTTTTAGACAGGAGCAATTCTGAAT	4	0.16051364365971107	No Hit
TGTCAGTGTGACCTTCTCATGTCAG	4	0.16051364365971107	No Hit
CACCCAACCATGTCCAAAATTGTAA	4	0.16051364365971107	No Hit
ACACTGACATGAGAAGGTCAAACTG	4	0.16051364365971107	No Hit
GGAATGGAATTGCCATCCCATAGTA	4	0.16051364365971107	No Hit
TATCAACGCAGAGTACATGGGATGG	4	0.16051364365971107	No Hit
CCCCTCTGCTGACTCCTTCTGGGGA	4	0.16051364365971107	No Hit
GGATAATTATGGGTTAGAGTTTTTT	4	0.16051364365971107	No Hit
GGGGATAGCTCCCAGAAACTCCCAG	4	0.16051364365971107	No Hit
ACGCAGAGTACATGGGATGGGTGGG	4	0.16051364365971107	No Hit
GATGTACAGAGGATCAGGAAATTGA	4	0.16051364365971107	No Hit
GGACAGTATGACCCACTGGGGCAAT	4	0.16051364365971107	No Hit
CTTCAGGCCCTTTGCCCAGCACTTG	4	0.16051364365971107	No Hit
ATCCAGAGGTTAGGCGTGGCCCCCT	4	0.16051364365971107	No Hit
GAACAGAGGTGTGTAGCAATGGGGG	4	0.16051364365971107	No Hit
GGTCACACTGACATGAGAAGGTCAA	4	0.16051364365971107	No Hit
CATTGCCCCAGTGGGTCATACTGTC	4	0.16051364365971107	No Hit
CACTTGTCCTGCATTCTCCCCAGAA	3	0.1203852327447833	No Hit
GTATCAACGCAGAGTACATGGGGTG	3	0.1203852327447833	No Hit
GTACACAGGAGACATCCAAGCTGGC	3	0.1203852327447833	No Hit
GTACATGGGGGATCCCCCATGTGCC	3	0.1203852327447833	No Hit
GTATCAACGCAGAGTACATGGGGCC	3	0.1203852327447833	No Hit
GGGCTAGGTGGTGGACCATAGAGCA	3	0.1203852327447833	No Hit
GGACAGGCCAGCTTGGATGTCTCCT	3	0.1203852327447833	No Hit
GTATCAACGCAGAGTACATGGGCCC	3	0.1203852327447833	No Hit
AGTTTGGTGTGGCCGGGACCTAGTG	3	0.1203852327447833	No Hit
GGGTTCTCAGAGGCTCTTGTTTTCC	3	0.1203852327447833	No Hit
CCTCTGGAGGTAAGAGGTAGAGGGG	3	0.1203852327447833	No Hit
GGACAACATGCCCTGTGGGAAAATG	3	0.1203852327447833	No Hit
CCCCTGAGGGCCAGCAGAAAAAAAT	3	0.1203852327447833	No Hit
CAGTGTGACCTTCTCATGTCAGTGT	3	0.1203852327447833	No Hit
GTATCAACGCAGAGTACATGGGGAA	3	0.1203852327447833	No Hit
GAACTGGGGATAGCTCCCAGAAACT	3	0.1203852327447833	No Hit
CTGGTGAGAAATCCCGAGTCACTCT	3	0.1203852327447833	No Hit
GGAGTAAACCCCTGAGGGCCAGCAG	3	0.1203852327447833	No Hit
GAGAGAGTCTGGCTAGCTCAGGATG	3	0.1203852327447833	No Hit
CCAGTGGGTCATACTGTCCTGAGAA	3	0.1203852327447833	No Hit
ATGCCCAACAGTGGGGAAAGGGAAC	3	0.1203852327447833	No Hit
TATCAACGCAGAGTACATGGGATCC	3	0.1203852327447833	No Hit
ACTCTGTGGATAGCATTTTCCCACA	3	0.1203852327447833	No Hit
CTAAGGTCCCTCCCTTTGAGACCTG	3	0.1203852327447833	No Hit
CCTTAGAGGAAATGCCCAACAGTGG	3	0.1203852327447833	No Hit
GACCTGGGAGGTGAGAGATGCTCAG	3	0.1203852327447833	No Hit
TATCAACGCAGAGTACATGGGGAAG	3	0.1203852327447833	No Hit
GCTTGGATGTCTCCTGTGTACTCTG	3	0.1203852327447833	No Hit
CATCTAACCTCCTGCTGGAAGGCCT	3	0.1203852327447833	No Hit
ATTTCAGCTAATGTCATACCTGTAG	3	0.1203852327447833	No Hit
TCACATGGCCATGGCCGACCTCTCT	3	0.1203852327447833	No Hit
GGTATCAACGCAGAGTACATGGGGA	3	0.1203852327447833	No Hit
GGAGGGGGGATCAGGAGTAAACCCC	3	0.1203852327447833	No Hit
ATCAACGCAGAGTACATGGGATCCC	3	0.1203852327447833	No Hit
GTCTAAAAGAAATAGAGGGATGAAG	3	0.1203852327447833	No Hit
GATCCCTAGGTGGGTCAGTCTCGGG	3	0.1203852327447833	No Hit
GTACATGGGGCCTCTCCTAGCATGG	3	0.1203852327447833	No Hit
GTACATGGGGTGAGGAGGAAGAGAT	3	0.1203852327447833	No Hit
CTACAGGTATGACATTAGCTGAAAT	3	0.1203852327447833	No Hit
GGTTAGGCGTGGCCCCCTGGTTGAA	3	0.1203852327447833	No Hit
AAATATCGATATCAGGATATTCAGA	3	0.1203852327447833	No Hit
CTCTACAAGTTCCCTTTCCCCACTG	3	0.1203852327447833	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	pass
>>END_MODULE