FastQCFastQC Report
Thu 2 Feb 2017
SRR4063938_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063938_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2492
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGT210.8426966292134831No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGG150.6019261637239166No Hit
AGCATGGACAGGCCAGCTTGGATGTCTCCTGTGTACTCTGTGGATAGCAT110.44141252006420545No Hit
CCTGTAGGGTTCTCAGAGGCTCTTGTTTTCCTGGCATCTGGGAGTTTCTG100.40128410914927765No Hit
GACATGAGAAGGTCAAACTGACATGAGAAGGTCACACTGACATGAGAAGG100.40128410914927765No Hit
TGTCATACCTGTAGGGTTCTCAGAGGCTCTTGTTTTCCTGGCATCTGGGA90.3611556982343499No Hit
GTGTAGCAATGGGGGATGGGGAACTGGGGATAGCTCCCAGAAACTCCCAG90.3611556982343499No Hit
CTCCTAGCATGGACAGGCCAGCTTGGATGTCTCCTGTGTACTCTGTGGAT90.3611556982343499No Hit
TCTCATGTCAGTGTGACCTTCTCATGTCAGTTTGACCTTCTCATGTCAGT90.3611556982343499No Hit
GACATGAGAAGGTCAAACTGCCCCAGGGGCTTCAGGCCCTTTGCCCAGCA90.3611556982343499No Hit
GTATCAACGCAGAGTACATGGGATGGGTGGGCTAGGTGGTGGACCATAGA80.32102728731942215No Hit
GTGCTGGGCAAAGGGCCTGAAGCCCCTGGGGCAGTTTGACCTTCTCATGT80.32102728731942215No Hit
CTTCTGGGGAGAATGCAGGACAAGTGCTGGGCAAAGGGCCTGAAGCCCCT70.2808988764044944No Hit
GTATGACCCACTGGGGCAATGTGACCCTCAGGACAACATGCCCTGTGGGA70.2808988764044944No Hit
TCTCAGGACAGTATGACCCACTGGGGCAATGTGACCCTCAGGACAACATG70.2808988764044944No Hit
GTATCAACGCAGAGTACATGGGGCCTCTCCTAGCATGGACAGGCCAGCTT70.2808988764044944No Hit
ATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTT70.2808988764044944Illumina PCR Primer Index 10 (95% over 22bp)
CCATAGAGCATCCTCCCCTCTGCTGACTCCTTCTGGGGAGAATGCAGGAC70.2808988764044944No Hit
TATCAACGCAGAGTACATGGGATGGGTGGGCTAGGTGGTGGACCATAGAG60.2407704654895666No Hit
GTAATAAGACAGGGCATCAAGTGGAGGAATGGAATTGCCATCCCATAGTA60.2407704654895666No Hit
ACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTCTTCT60.2407704654895666Illumina PCR Primer Index 10 (95% over 24bp)
GTATCAACGCAGAGTACATGGGACCTCCTTTCCCTCAGTCTCTTCTCCAC60.2407704654895666No Hit
ATTCTGAATTACAATTTTGGACATGGTTGGGTGACCCCATCTTTCAACCA60.2407704654895666No Hit
CTCTAGAATATCCCAAAGACCTGGGAGGTGAGAGATGCTCAGGTCTCAAA60.2407704654895666No Hit
GTGGTGGACCATAGAGCATCCTCCCCTCTGCTGACTCCTTCTGGGGAGAA60.2407704654895666No Hit
GACATGAGAAGGTCACACTGACATGAGAAGGTCAAACTGACATGAGAAGG50.20064205457463882No Hit
CTCTACAAGTTCCCTTTCCCCACTGTTGGGCATTTCCTCTAAGGTCCCTC50.20064205457463882No Hit
CCTCTGGATATGGTCCCCACAGGCTCGCTCTCCCCTTGTAGGCTATTTCA50.20064205457463882No Hit
GGTATGACATTAGCTGAAATAGCCTACAAGGGGAGAGCGAGCCTGTGGGG50.20064205457463882No Hit
GACATGAGAAGGTCACACTGACATGAGAAGGTCAAACTGCCCCAGGGGCT50.20064205457463882No Hit
ACCATAGAGCATCCTCCCCTCTGCTGACTCCTTCTGGGGAGAATGCAGGA50.20064205457463882No Hit
AGCCTGTGGGGACCATATCCAGAGGTTAGGCGTGGCCCCCTGGTTGAAAG50.20064205457463882No Hit
GTCCTGAGAAGGTCACACTGACATGAGAAGGTCACACTGACATGAGAAGG50.20064205457463882No Hit
ATGCAGGACAAGTGCTGGGCAAAGGGCCTGAAGCCCCTGGGGCAGTTTGA40.16051364365971107No Hit
CTTTTAGACAGGAGCAATTCTGAATTACAATTTTGGACATGGTTGGGTGA40.16051364365971107No Hit
CCTGTGTACTCTGTGGATAGCATTTTCCCACAGGGCATGTTGTCCTGAGG40.16051364365971107No Hit
CCTTAGAGGAAATGCCCAACAGTGGGGAAAGGGAACTTGTAGAGTCCACC40.16051364365971107No Hit
ACGCAGAGTACATGGGGTGAGGAGGAAGAGATGTACAGAGGATCAGGAAA40.16051364365971107No Hit
GTCTCGGGATGGCCTTTCCTTCAGTTTTTGTTCCACTCTTCATCCCTCTA40.16051364365971107No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCGCGTATGCCGG40.16051364365971107No Hit
GTACATGGGGTGAGGAGGAAGAGATGTACAGAGGATCAGGAAATTGAACA40.16051364365971107No Hit
TTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTC40.16051364365971107No Hit
GTACATGGGATGGGTGGGCTAGGTGGTGGACCATAGAGCATCCTCCCCTC40.16051364365971107No Hit
GTATCAACGCAGAGTACATGGGGTGAGGAGGAAGAGATGTACAGAGGATC40.16051364365971107No Hit
GGTCAAACTGCCCCAGGGGCTTCAGGCCCTTTGCCCAGCACTTGTCCTGC40.16051364365971107No Hit
ATTTCAGCTAATGTCATACCTGTAGGGTTCTCAGAGGCTCTTGTTTTCCT30.1203852327447833No Hit
GGGATGAAGAGTGGAACAAAAACTGAAGGAAAGGCCATCCCGAGACTGAC30.1203852327447833No Hit
CGCCTAACCTCTGGATATGGTCCCCACAGGCTCGCTCTCCCCTTGTAGGC30.1203852327447833No Hit
GCCCAGCACTTGTCCTGCATTCTCCCCAGAAGGAGTCAGCAGAGGGGAGG30.1203852327447833No Hit
AGTTTGACCTTCTCATGTCAGTGTGACCTTCTCATGTCAGTGTGACCTTC30.1203852327447833No Hit
GTCCTGAGGGTCACATTGCCCCAGTGGGTCATACTGTCCTGAGAAGGTCA30.1203852327447833No Hit
CTACACACCTCTGTTCAATTTCCTGATCCTCTGTACATCTCTTCCTCCTC30.1203852327447833No Hit
CTATTTCCAAGGCTATAGCTCGATTTAACCATTCCTTGGCCAAGTTTACC30.1203852327447833No Hit
CCTCTAAGGTCCCTCCCTTTGAGACCTGAGCATCTCTCACCTCCCAGGTC30.1203852327447833No Hit
TGTAAGATTTTACCTGAAAGCACTGAGGAAGCAACTCTCTGCCCTGCCAT30.1203852327447833No Hit
GGCTTCAGGCCCTTTGCCCAGCACTTGTCCTGCATTCTCCCCAGAAGGAG30.1203852327447833No Hit
ACTCTACAAGTTCCCTTTCCCCACTGTTGGGCATTTCCTCTAAGGTCCCT30.1203852327447833No Hit
CTAGGTGGGTCAGTCTCGGGATGGCCTTTCCTTCAGTTTTTGTTCCACTC30.1203852327447833No Hit
CCTCAGGACAACATGCCCTGTGGGAAAATGCTATCCACAGAGTACACAGG30.1203852327447833No Hit
GTGTGTAGCAATGGGGGATGGGGAACTGGGGATAGCTCCCAGAAACTCCC30.1203852327447833No Hit
CTCTACCTCTTACCTCCAGAGGTTGCCTGTTTCTATTTTTTTCTGCTGGC30.1203852327447833No Hit
ATACACATCTCCGAGCCCACGAGACCGGAGCCTATCGCGTATGCCGTGGT30.1203852327447833No Hit
GGTGTGTAGCAATGGGGGATGGGGAACTGGGGATAGCTCCCAGAAACTCC30.1203852327447833No Hit
ACCATATCCAGAGGTTAGGCGTGGCCCCCTGGTTGAAAGATGGGGTCACC30.1203852327447833No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGGCGT30.1203852327447833No Hit
GCTAGGTGGTGGACCATAGAGCATCCTCCCCTCTGCTGACTCCTTCTGGG30.1203852327447833No Hit
CCTCTGGAGGTAAGAGGTAGAGGGGCCCTCTAGAATATCCCAAAGACCTG30.1203852327447833No Hit
TCTCATGTCAGTGTGACCTTCTCAGGACAGTATGACCCACTGGGGCAATG30.1203852327447833No Hit
TTACAATTTTGGACATGGTTGGGTGACCCCATCTTTCAACCAGGGGGCCA30.1203852327447833No Hit
TCCCAGGTCTTTGGGATATTCTAGAGGGCCCCTCTACCTCTTACCTCCAG30.1203852327447833No Hit
ATACACATCTCCGAGCCCACGAGACCGGAGCCTATCTCGTATGCCGTGTT30.1203852327447833No Hit
CCCAACCATGTCCAAAATTGTAATTCAGAATTGCTCCTGTCTAAAAGAAA30.1203852327447833No Hit
ACGCAGAGTACATGGGGCCTCTCCTAGCATGGACAGGCCAGCTTGGATGT30.1203852327447833No Hit
CCCTACAGGTATGACATTAGCTGAAATAGCCTACAAGGGGAGAGCGAGCC30.1203852327447833No Hit
CCTCTACCTCTTACCTCCAGAGGTTGCCTGTTTCTATTTTTTTCTGCTGG30.1203852327447833No Hit
CATCTAACCTCCTGCTGGAAGGCCTTCCTGCGCTCCAGTCACGGCTGCCA30.1203852327447833No Hit
GAGCATCCTCCCCTCTGCTGACTCCTTCTGGGGAGAATGCAGGACAAGTG30.1203852327447833No Hit
GGTCCCCACAGGCTCGCTCTCCCCTTGTAGGCTATTTCAGCTAATGTCAT30.1203852327447833No Hit
CTCCTGTGTACTCTGTGGATAGCATTTTCCCACAGGGCATGTTGTCCTGA30.1203852327447833No Hit
GTGAAACCAAAGAAAACTGTCCCAACGACATTTGAATTTACAGATATCGC30.1203852327447833No Hit
GATGAAGAGTGGAACAAAAACTGAAGGAAAGGCCATCCCGAGACTGACCC30.1203852327447833No Hit
GTACATGGGGAAGGGGGAACATGAAAAGGAGGGGGGATCAGGAGTAAACC30.1203852327447833No Hit
CAGCTAATGTCATACCTGTAGGGTTCTCAGAGGCTCTTGTTTTCCTGGCA30.1203852327447833No Hit
GGGCATGTTGTCCTGAGGGTCACATTGCCCCAGTGGGTCATACTGTCCTG30.1203852327447833No Hit
TCTCATGTCAGTGTGACCTTCTCATGTCAGTGTGACCTTCTCAGGACAGT30.1203852327447833No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers