FastQCFastQC Report
Thu 2 Feb 2017
SRR4063937_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063937_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126037
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT37562.9800772788942926No Hit
GTACTTTTTTTTTTTTTTTTTTTTT34712.753953204217809No Hit
TATCAACGCAGAGTACTTTTTTTTT30382.41040329427073No Hit
GAGTACTTTTTTTTTTTTTTTTTTT23241.8439029808706968No Hit
GTACATGGGGTGGTATCAACGCAAA16681.3234209002118424No Hit
ACGCAGAGTACTTTTTTTTTTTTTT16611.3178669755706658No Hit
GGTATCAACGCAGAGTACTTTTTTT13721.0885692296706524No Hit
GTACATGGAAGCAGTGGTATCAACG13021.0330299832588843No Hit
TCCATGTACTCTGCGTTGATACCAC11190.8878345247824052No Hit
GTATCAACGCAGAGTACATGGGGTG9070.7196299499353365No Hit
GTACATGGGTGGTATCAACGCAAAA8570.6799590596412165No Hit
GCAGAGTACTTTTTTTTTTTTTTTT7970.6323539912882725No Hit
GTACATGGGAGTGGTATCAACGCAA7400.5871291763529758No Hit
GTACATGGGAAGCAGTGGTATCAAC6720.5331767655529726No Hit
TATCAACGCAGAGTACATGGGGTGG6690.5307965121353253No Hit
CCCATGTACTCTGCGTTGATACCAC6670.5292096765235605No Hit
GTATCAACGCAGAGTACATGGGAAG5480.434792957623555No Hit
GGTATCAACGCAGAGTACATGGGGT5170.4101970056412006No Hit
CATGTACTCTGCGTTGATACCACTG5160.4094035878353182No Hit
GAGTACATGGAAGCAGTGGTATCAA5100.40464308100002383No Hit
ATCAACGCAGAGTACTTTTTTTTTT4530.35941826606472704No Hit
GTATCAACGCAGAGTACATGGGAGT4390.34831041678237346No Hit
ACGCAGAGTACATGGGGTGGTATCA3760.2983250950117823No Hit
TATCAACGCAGAGTACATGGGAAGC3570.2832501567000167No Hit
GGTATCAACGCAGAGTACATGGGAA3210.25468711568825025No Hit
CATGGAAGCAGTGGTATCAACGCAG3110.24675293762942627No Hit
GCTTCCATGTACTCTGCGTTGATAC3110.24675293762942627No Hit
GTATCAACGCAGAGTACATGGGTGG2980.23643850615295506No Hit
GCGTTGATACCACTGCTTCCATGTA2780.2205701500353071No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT2690.2134293897823655No Hit
TATCAACGCAGAGTACATGGGAGTG2650.2102557185588359No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT2530.2007347048882471No Hit
GAGTACATGGGGTGGTATCAACGCA2460.1951807802470703No Hit
GCGTTGATACCACTGCTTCCCATGT2340.1856597665764815No Hit
ACTCTGCGTTGATACCACTGCTTCC2340.1856597665764815No Hit
GTATCAACGCAGAGTACATGGAAGC2300.18248609535295193No Hit
TATCAACGCAGAGTACATGGGTGGT2200.17455191729412792No Hit
GAGTACATGGGAAGCAGTGGTATCA2190.1737584994882455No Hit
GTACATGGGCAGTGGTATCAACGCA2100.16661773923530393No Hit
GGTATCAACGCAGAGTACATGGGAG2070.1642374858176567No Hit
GTATCAACGCAGAGTACATGGGCAG2070.1642374858176567No Hit
CAGTGGTATCAACGCAGAGTACATG1780.14122836944706713No Hit
ACGCAGAGTACATGGGAAGCAGTGG1740.13805469822353753No Hit
ATCAACGCAGAGTACATGGGGTGGT1730.13726128041765515No Hit
GTATCAACGCAGAGTACATGGGGTA1720.13646786261177274No Hit
GTACATGGGGTATCAACGCAAAAAA1700.13488102700000792No Hit
AAAAAGTACTCTGCGTTGATACCAC1650.13091393797059594No Hit
CAACGCAGAGTACTTTTTTTTTTTT1640.13012052016471354No Hit
TATCAACGCAGAGTACATGGAAGCA1530.12139292430000713No Hit
TCAACGCAGAGTACTTTTTTTTTTT1500.11901267088235994No Hit
GGTGGTATCAACGCAAAAAAAAAAA1470.11663241746471276No Hit
ACGCAGAGTACATGGAAGCAGTGGT1460.11583899965883035No Hit
AACGCAGAGTACTTTTTTTTTTTTT1450.11504558185294796No Hit
GCTTCCCATGTACTCTGCGTTGATA1410.11187191062941836No Hit
GGTATCAACGCAGAGTACATGGGTG1400.11107849282353595No Hit
ACGCAGAGTACATGGGTGGTATCAA1370.10869823940588876No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG1340.10631798598824155No Hit
ACGCAGAGTACATGGGAGTGGTATC1320.10473115037647675No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGGTGT401.2699606E-519.06
TAAAACA250.00601581418.992464
TATCACC250.00601581418.992463
GGGTGTG453.497414E-516.888897
TCCTACA653.3267952E-614.6095852
GTCCTAC602.503168E-514.2669921
CTCATTT602.5315067E-514.24999919
CCTACAG602.5410158E-514.2443453
GTAGAAC500.00149070613.2947233
TGTAGAA500.00149070613.2947232
GGAGTGG1102.6448106E-912.9545468
TAGAACA604.0594244E-412.661644
AAGTACT604.0594244E-412.661644
TCTCATT701.0756363E-412.21428618
AAAGTAC550.003050684212.0861113
GTGTGGT802.8176992E-511.8759
TCCATGT2750.011.7594591
TTCTCAT752.0451262E-411.40000117
TACAGTG951.3328148E-511.05
GGGAGTG1303.1473974E-810.9615387
Produced by FastQC (version 0.11.5)