##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063937_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	126037
Sequences flagged as poor quality	0
Sequence length	25
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.39570126232773	32.0	32.0	32.0	27.0	32.0
2	30.603552924934743	32.0	32.0	32.0	32.0	32.0
3	30.71744805096916	32.0	32.0	32.0	32.0	32.0
4	30.53549354554615	32.0	32.0	32.0	32.0	32.0
5	30.77080539841475	32.0	32.0	32.0	32.0	32.0
6	34.21423074176631	36.0	36.0	36.0	32.0	36.0
7	33.845711973468106	36.0	36.0	36.0	32.0	36.0
8	33.61688234407357	36.0	36.0	36.0	27.0	36.0
9	33.52271158469338	36.0	36.0	36.0	21.0	36.0
10	33.701476550536746	36.0	36.0	36.0	32.0	36.0
11	34.05375405634853	36.0	36.0	36.0	32.0	36.0
12	33.888850099573936	36.0	36.0	36.0	32.0	36.0
13	33.89447543181764	36.0	36.0	36.0	32.0	36.0
14	33.859231812880346	36.0	36.0	36.0	32.0	36.0
15	33.966993819275295	36.0	36.0	36.0	32.0	36.0
16	33.93370994231852	36.0	36.0	36.0	32.0	36.0
17	33.8392773550624	36.0	36.0	36.0	32.0	36.0
18	33.86210398533764	36.0	36.0	36.0	32.0	36.0
19	33.75527027777557	36.0	36.0	36.0	32.0	36.0
20	33.53568396581956	36.0	36.0	36.0	27.0	36.0
21	33.443798249720324	36.0	36.0	36.0	21.0	36.0
22	33.5089695882955	36.0	36.0	36.0	27.0	36.0
23	33.58894610312845	36.0	36.0	36.0	27.0	36.0
24	33.63786824503916	36.0	36.0	36.0	27.0	36.0
25	33.17698770995818	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	1.0
5	2.0
6	4.0
7	4.0
8	14.0
9	6.0
10	11.0
11	2.0
12	6.0
13	2.0
14	40.0
15	68.0
16	105.0
17	132.0
18	200.0
19	273.0
20	354.0
21	543.0
22	652.0
23	970.0
24	1214.0
25	1586.0
26	2000.0
27	2385.0
28	3027.0
29	3791.0
30	4739.0
31	6395.0
32	8527.0
33	11755.0
34	24487.0
35	52742.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	51.58998539404331	16.8801994030609	13.233473042484285	18.29634216041151
2	13.209448469027462	23.18397854496116	41.83018463711309	21.776388348898287
3	19.329551315110884	34.397587971595186	27.665331058832866	18.607529654461064
4	10.110130760441791	16.17367017900216	35.77742160721087	37.93877745334518
5	10.162901600450697	42.96142890015632	30.943369278011858	15.932300221381132
6	26.273230808297228	36.49796060880192	21.628656223714074	15.600152359186783
7	25.128762687787187	34.10445451443174	22.75984667518471	18.006936122596365
8	30.970655702572486	28.290220419567095	21.47602530419805	19.26309857366236
9	30.701266948210716	12.463087035214174	21.453180071761977	35.382465944813134
10	17.35589022173178	28.439303605026872	30.54944705985091	23.65535911339044
11	31.96179921566137	24.397853389009732	23.958052172808536	19.682295222520363
12	25.645687608673214	27.201054378289967	31.01920588165239	16.134052131384426
13	31.578362327010566	21.883014283786036	29.012997530707363	17.52562585849603
14	24.881689693504843	19.803874861044942	32.3749404478323	22.939494997617913
15	23.794250510086616	25.89810970236347	32.79003485261077	17.517604934939147
16	19.246954947515523	27.63335503644535	31.56452970414953	21.5551603118896
17	17.561688208580776	26.132935310743434	32.50182603448823	23.803550446187558
18	18.873527987104254	24.651997490729197	37.22773220680838	19.24674231535817
19	20.490078530082023	25.90222250454585	36.17386194903882	17.433837016333303
20	23.16121296818301	21.77879767188877	37.09097261372569	17.969016746202527
21	25.226920786182248	22.01072066706373	32.81238832638475	19.949970220369266
22	22.348475825214194	24.037415533163404	33.35239048095476	20.261718160667634
23	20.053998252997697	25.861192726117682	34.60255697609783	19.482252044786787
24	19.29062584364231	28.110558828667166	34.23639453081577	18.36242079687475
25	20.97385117483106	25.55882890108232	34.19754949060215	19.269770433484474
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	152.0
1	152.0
2	430.5
3	709.0
4	709.0
5	709.0
6	2257.0
7	3805.0
8	3805.0
9	3805.0
10	3296.0
11	2787.0
12	2787.0
13	2787.0
14	1695.5
15	604.0
16	604.0
17	604.0
18	1233.0
19	1862.0
20	1862.0
21	1862.0
22	2826.5
23	3791.0
24	3791.0
25	3791.0
26	5705.0
27	7619.0
28	7619.0
29	7619.0
30	8668.5
31	9718.0
32	9718.0
33	9718.0
34	8964.5
35	8211.0
36	8211.0
37	8211.0
38	8160.0
39	8109.0
40	8109.0
41	8109.0
42	10004.5
43	11900.0
44	11900.0
45	11900.0
46	17507.5
47	23115.0
48	23115.0
49	23115.0
50	20629.0
51	18143.0
52	18143.0
53	18143.0
54	13363.0
55	8583.0
56	8583.0
57	8583.0
58	7354.5
59	6126.0
60	6126.0
61	6126.0
62	5267.5
63	4409.0
64	4409.0
65	4409.0
66	3647.5
67	2886.0
68	2886.0
69	2886.0
70	2285.0
71	1684.0
72	1684.0
73	1684.0
74	1306.0
75	928.0
76	928.0
77	928.0
78	698.0
79	468.0
80	468.0
81	468.0
82	340.5
83	213.0
84	213.0
85	213.0
86	130.0
87	47.0
88	47.0
89	47.0
90	31.5
91	16.0
92	16.0
93	16.0
94	12.5
95	9.0
96	9.0
97	9.0
98	76.0
99	143.0
100	143.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.04839848615882637
2	0.004760506835294397
3	0.0015868356117647992
4	0.003967089029411998
5	0.007934178058823996
6	0.015074938311765592
7	0.023802534176471987
8	0.03967089029411998
9	0.051572157382355974
10	0.05871291763529757
11	0.056332664217650374
12	0.06823393130588637
13	0.07140760252941597
14	0.07696152717059276
15	0.06188658885882717
16	0.07537469155882796
17	0.06426684227647436
18	0.08251545181176956
19	0.07775494497647516
20	0.07775494497647516
21	0.08886279425882876
22	0.07934178058823996
23	0.08489570522941677
24	0.07537469155882796
25	0.08251545181176956
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	126037.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.12163888381983
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.72877081240514	25.884462499107403
2	15.506444097719452	11.51249236335362
3	5.943958791972129	6.6194847544768605
4	2.9132023852779616	4.325713877670843
5	1.6136961121679099	2.9951522172060585
6	1.0323380426186761	2.2993248014471943
7	0.649753136555026	1.6883930909177465
8	0.4488426272255114	1.3329419138824314
9	0.34197533502896105	1.1425216404706555
>10	1.5474383910060487	9.796329649229989
>50	0.12824075063586038	3.2339709767766607
>100	0.1025926005086883	7.658862080182803
>500	0.02351080428324107	5.871291763529757
>1k	0.019236112595379058	15.63905837174798
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3756	2.9800772788942926	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	3471	2.753953204217809	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3038	2.41040329427073	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2324	1.8439029808706968	No Hit
GTACATGGGGTGGTATCAACGCAAA	1668	1.3234209002118424	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1661	1.3178669755706658	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1372	1.0885692296706524	No Hit
GTACATGGAAGCAGTGGTATCAACG	1302	1.0330299832588843	No Hit
TCCATGTACTCTGCGTTGATACCAC	1119	0.8878345247824052	No Hit
GTATCAACGCAGAGTACATGGGGTG	907	0.7196299499353365	No Hit
GTACATGGGTGGTATCAACGCAAAA	857	0.6799590596412165	No Hit
GCAGAGTACTTTTTTTTTTTTTTTT	797	0.6323539912882725	No Hit
GTACATGGGAGTGGTATCAACGCAA	740	0.5871291763529758	No Hit
GTACATGGGAAGCAGTGGTATCAAC	672	0.5331767655529726	No Hit
TATCAACGCAGAGTACATGGGGTGG	669	0.5307965121353253	No Hit
CCCATGTACTCTGCGTTGATACCAC	667	0.5292096765235605	No Hit
GTATCAACGCAGAGTACATGGGAAG	548	0.434792957623555	No Hit
GGTATCAACGCAGAGTACATGGGGT	517	0.4101970056412006	No Hit
CATGTACTCTGCGTTGATACCACTG	516	0.4094035878353182	No Hit
GAGTACATGGAAGCAGTGGTATCAA	510	0.40464308100002383	No Hit
ATCAACGCAGAGTACTTTTTTTTTT	453	0.35941826606472704	No Hit
GTATCAACGCAGAGTACATGGGAGT	439	0.34831041678237346	No Hit
ACGCAGAGTACATGGGGTGGTATCA	376	0.2983250950117823	No Hit
TATCAACGCAGAGTACATGGGAAGC	357	0.2832501567000167	No Hit
GGTATCAACGCAGAGTACATGGGAA	321	0.25468711568825025	No Hit
CATGGAAGCAGTGGTATCAACGCAG	311	0.24675293762942627	No Hit
GCTTCCATGTACTCTGCGTTGATAC	311	0.24675293762942627	No Hit
GTATCAACGCAGAGTACATGGGTGG	298	0.23643850615295506	No Hit
GCGTTGATACCACTGCTTCCATGTA	278	0.2205701500353071	No Hit
ACTTTTTTTTTTTTTTTTTTTTTTT	269	0.2134293897823655	No Hit
TATCAACGCAGAGTACATGGGAGTG	265	0.2102557185588359	No Hit
CTTTTTTTTTTTTTTTTTTTTTTTT	253	0.2007347048882471	No Hit
GAGTACATGGGGTGGTATCAACGCA	246	0.1951807802470703	No Hit
GCGTTGATACCACTGCTTCCCATGT	234	0.1856597665764815	No Hit
ACTCTGCGTTGATACCACTGCTTCC	234	0.1856597665764815	No Hit
GTATCAACGCAGAGTACATGGAAGC	230	0.18248609535295193	No Hit
TATCAACGCAGAGTACATGGGTGGT	220	0.17455191729412792	No Hit
GAGTACATGGGAAGCAGTGGTATCA	219	0.1737584994882455	No Hit
GTACATGGGCAGTGGTATCAACGCA	210	0.16661773923530393	No Hit
GGTATCAACGCAGAGTACATGGGAG	207	0.1642374858176567	No Hit
GTATCAACGCAGAGTACATGGGCAG	207	0.1642374858176567	No Hit
CAGTGGTATCAACGCAGAGTACATG	178	0.14122836944706713	No Hit
ACGCAGAGTACATGGGAAGCAGTGG	174	0.13805469822353753	No Hit
ATCAACGCAGAGTACATGGGGTGGT	173	0.13726128041765515	No Hit
GTATCAACGCAGAGTACATGGGGTA	172	0.13646786261177274	No Hit
GTACATGGGGTATCAACGCAAAAAA	170	0.13488102700000792	No Hit
AAAAAGTACTCTGCGTTGATACCAC	165	0.13091393797059594	No Hit
CAACGCAGAGTACTTTTTTTTTTTT	164	0.13012052016471354	No Hit
TATCAACGCAGAGTACATGGAAGCA	153	0.12139292430000713	No Hit
TCAACGCAGAGTACTTTTTTTTTTT	150	0.11901267088235994	No Hit
GGTGGTATCAACGCAAAAAAAAAAA	147	0.11663241746471276	No Hit
ACGCAGAGTACATGGAAGCAGTGGT	146	0.11583899965883035	No Hit
AACGCAGAGTACTTTTTTTTTTTTT	145	0.11504558185294796	No Hit
GCTTCCCATGTACTCTGCGTTGATA	141	0.11187191062941836	No Hit
GGTATCAACGCAGAGTACATGGGTG	140	0.11107849282353595	No Hit
ACGCAGAGTACATGGGTGGTATCAA	137	0.10869823940588876	No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG	134	0.10631798598824155	No Hit
ACGCAGAGTACATGGGAGTGGTATC	132	0.10473115037647675	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGGGTGT	40	1.2699606E-5	19.0	6
TAAAACA	25	0.006015814	18.99246	4
TATCACC	25	0.006015814	18.99246	3
GGGTGTG	45	3.497414E-5	16.88889	7
TCCTACA	65	3.3267952E-6	14.609585	2
GTCCTAC	60	2.503168E-5	14.266992	1
CTCATTT	60	2.5315067E-5	14.249999	19
CCTACAG	60	2.5410158E-5	14.244345	3
GTAGAAC	50	0.001490706	13.294723	3
TGTAGAA	50	0.001490706	13.294723	2
GGAGTGG	110	2.6448106E-9	12.954546	8
TAGAACA	60	4.0594244E-4	12.66164	4
AAGTACT	60	4.0594244E-4	12.66164	4
TCTCATT	70	1.0756363E-4	12.214286	18
AAAGTAC	55	0.0030506842	12.086111	3
GTGTGGT	80	2.8176992E-5	11.875	9
TCCATGT	275	0.0	11.759459	1
TTCTCAT	75	2.0451262E-4	11.400001	17
TACAGTG	95	1.3328148E-5	11.0	5
GGGAGTG	130	3.1473974E-8	10.961538	7
>>END_MODULE