FastQCFastQC Report
Thu 2 Feb 2017
SRR4063937_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063937_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences126037
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA16601.3170735577647834No Hit
GTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAA8700.6902734911176877No Hit
TATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAA7720.6125185461412125No Hit
GTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7330.5815752517117989No Hit
GTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAA6860.5442846148353262No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6600.5236557518823838No Hit
TCCATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATA6470.5133413204059125No Hit
GTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATC5980.47446384791767493No Hit
ACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAA4810.3816339646294342No Hit
GGTATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAA4230.33561573188825505No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4180.33164864285884305No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3460.2745225608353103No Hit
GTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGGGAAGCAGTGGTA3230.25627395130001507No Hit
GAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA3110.24675293762942627No Hit
GTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAA2990.23723192395883747No Hit
TATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAA2980.23643850615295506No Hit
CCCATGTACTCTGCGTTGATACCACTGCTTCCCATGTACTCTGCGTTGAT2960.23485167054119027No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG2830.22453723906471912No Hit
GTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAA2620.2078754651411887No Hit
TATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA2600.2062886295294239No Hit
GAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTA2390.1896268556058935No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG2190.1737584994882455No Hit
CATGTACTCTGCGTTGATACCACTGCTTCCATGTACTCTGCGTTGATACC2180.17296508168236313No Hit
GTACATGGGCAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA2100.16661773923530393No Hit
ACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAA2010.15947697898236232No Hit
ATCAACGCAGAGTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAA1970.15630330775883272No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG1710.13567444480589033No Hit
GTATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATG1690.13408760919412555No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT1650.13091393797059594No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGG1620.12853368455294872No Hit
CATGGAAGCAGTGGTATCAACGCAGAGTACATGGAAGCAGTGGTATCAAC1570.12456659552353673No Hit
GGTATCAACGCAGAGTACATGGGAGTGGTATCAACGCAAAAAAAAAAAAA1560.12377317771765434No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGG1530.12139292430000713No Hit
ACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAA1520.12059950649412474No Hit
GTATCAACGCAGAGTACATGGAAGCAGTGGTATCAACGCAGAGTACATGG1480.11742583527059514No Hit
GGTATCAACGCAGAGTACATGGGTGGTATCAACGCAAAAAAAAAAAAAAA1430.11345874624118316No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1390.11028507501765356No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGT1380.10949165721177115No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGT1370.10869823940588876No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT1370.10869823940588876No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTT1360.10790482160000635No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGTT1310.10393773257059435No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGGTT1280.10155747915294715No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1280.10155747915294715No Hit
TATCAACGCAGAGTACATGGGAAGCAGTGGTATCAACGCAGAGTACATGG1270.10076406134706475No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCAAGAA300.005729540729.32936520
GGCAAGA300.005729540729.32936519
TATTAAG300.005729540729.3293652
ATACCAT300.005729540729.3293656
GGGGGTA407.0025295E-427.496287
TGGGTGG2500.022.8769046
ATGGGTG3150.022.3461825
CCTATAG500.002569767221.9970251
TTCTGGA500.002569767221.9970252
CATGGGT3650.020.4903774
TAACCCC654.9029983E-420.30494543
TAGGACG654.9029983E-420.3049454
AATCATG550.004454983420.00523234
CACTGTT550.004465237719.9972937
TGGTATA550.004465237719.99729344
ATGGGGG1001.1086086E-619.7973215
GGACGTG708.0756494E-418.8545916
AAATCAT600.007365102418.3381333
TGGGAGT2900.018.2044336
TGGGGGT750.001283351217.5976186
Produced by FastQC (version 0.11.5)