FastQCFastQC Report
Thu 2 Feb 2017
SRR4063936_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063936_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1285221
Sequences flagged as poor quality0
Sequence length25
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAAAGTGTGTATTTCTCATTT67840.5278469617287611No Hit
GTCCTACAGTGGACATTTCTAAATT57520.44754948759785285No Hit
CTGTAGGACGTGGAATATGGCAAGA56840.4422585687597697No Hit
CTTTAGGACGTGAAATATGGCGAGG55210.4295759250743646No Hit
GTATCAACGCAGAGTACTTTTTTTT42710.3323163876096018No Hit
GTCCTACAGTGTGCATTTCTCATTT38930.3029051034802575No Hit
CTGTAGGACCTGGAATATGGCGAGA30050.23381192806528994No Hit
ATTTAGAAATGTCCACTGTAGGACG27670.21529371213199908No Hit
TATCAACGCAGAGTACTTTTTTTTT27620.21490467398214003No Hit
CTGAAGGACCTGGAATATGGCGAGA26560.2066570652051281No Hit
GTCCTTCAGTGTGCATTTCTCATTT23210.1805915091645717No Hit
TTTCTAAATTTTCCACCTTTTTCAG21420.16666394339961765No Hit
GAATATGGCAAGAAAACTGAAAATC19100.14861257324615768No Hit
GTACTTTTTTTTTTTTTTTTTTTTT15580.12122428749608044No Hit
GGAATATGGCGAGAAAACTGAAAAT15260.11873444333698252No Hit
CTGTAGGACATGGAATATGGCAAGA14440.11235421767929407No Hit
GGTATCAACGCAGAGTACTTTTTTT14380.11188737189946321No Hit
GCCATATTCCACGTCCTACAGTGGA13530.10527372335185933No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATCGCC1100.017.27580815
CGGAATT402.756895E-416.62796415
TCGCGTA651.910339E-716.0785379
GTCGCGT651.9120307E-716.0772868
CGCATCG1200.015.83615713
ATCGCCA1200.015.83554216
CCGCATC1250.015.20271112
GCATCGC1250.015.20271114
TCGCCAG1300.014.61742217
GGGTCGC801.2886812E-714.2492116
CGCGTAA759.657997E-713.93473310
ACGATCA551.9580647E-413.8195719
CGAACGA903.630157E-813.72413416
CGCCAGT1400.013.57279118
GGTCGCG852.698307E-713.4115447
AGGCCCG2200.013.38770910
CCACCTT7400.013.2253313
ATCGCTC655.443923E-513.1556816
CGGTCCA2100.013.12036410
GTTTTCG801.994631E-613.06482915
Produced by FastQC (version 0.11.5)