##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063936_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1285221
Sequences flagged as poor quality	0
Sequence length	25
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.341103203262318	32.0	32.0	32.0	32.0	32.0
2	31.013722153621828	32.0	32.0	32.0	32.0	32.0
3	31.03052782361944	32.0	32.0	32.0	32.0	32.0
4	31.042763851508806	32.0	32.0	32.0	32.0	32.0
5	31.01915935080426	32.0	32.0	32.0	32.0	32.0
6	34.688226382855554	36.0	36.0	36.0	32.0	36.0
7	34.60136894744172	36.0	36.0	36.0	32.0	36.0
8	34.57716610606269	36.0	36.0	36.0	32.0	36.0
9	34.667118728996805	36.0	36.0	36.0	32.0	36.0
10	34.43135460749552	36.0	36.0	36.0	32.0	36.0
11	34.6549729579582	36.0	36.0	36.0	32.0	36.0
12	34.50832580544513	36.0	36.0	36.0	32.0	36.0
13	34.57419930113187	36.0	36.0	36.0	32.0	36.0
14	34.491754336413734	36.0	36.0	36.0	32.0	36.0
15	34.45933500930968	36.0	36.0	36.0	32.0	36.0
16	34.45281006146025	36.0	36.0	36.0	32.0	36.0
17	34.3980194845867	36.0	36.0	36.0	32.0	36.0
18	34.397731596355804	36.0	36.0	36.0	32.0	36.0
19	34.35378273464252	36.0	36.0	36.0	32.0	36.0
20	34.34607433274122	36.0	36.0	36.0	32.0	36.0
21	34.31848919368731	36.0	36.0	36.0	32.0	36.0
22	34.29724615455241	36.0	36.0	36.0	32.0	36.0
23	34.263131399191266	36.0	36.0	36.0	32.0	36.0
24	34.24026295866625	36.0	36.0	36.0	32.0	36.0
25	33.893359196589536	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	16.0
5	12.0
6	44.0
7	17.0
8	76.0
9	69.0
10	131.0
11	51.0
12	76.0
13	72.0
14	127.0
15	214.0
16	347.0
17	463.0
18	559.0
19	675.0
20	1028.0
21	1405.0
22	2263.0
23	3351.0
24	5206.0
25	7829.0
26	11386.0
27	15503.0
28	21586.0
29	29598.0
30	40187.0
31	55246.0
32	80227.0
33	116155.0
34	264905.0
35	626395.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.35327816726822	20.42426134112539	12.522193430977943	24.700267060628445
2	12.676934186046331	21.51378084943972	45.29110541702039	20.518179547493567
3	17.348455257477834	28.42931594976027	30.109757407029893	24.112471385732004
4	10.338849585892005	17.613906932301667	40.627324591572176	31.41991889023415
5	10.918978983138421	40.72014012797338	36.00871831304844	12.352162575839753
6	28.4463760446983	40.11050067701119	19.920081552612327	11.523041725678178
7	26.656191559055557	32.88187642952922	23.66214742818987	16.799784583225353
8	23.92970865534373	39.46404289649407	21.009334766537435	15.59691368162476
9	27.69891980930851	14.748775482878557	21.305741607425883	36.24656310038705
10	14.967476731557497	29.31251532739312	34.43791773228543	21.28209020876396
11	33.32422475912882	22.20335631496671	27.393453037125614	17.07896588877886
12	24.94822404584313	25.18506983914418	32.81427614880331	17.052429966209377
13	28.932136800848347	23.559575140651738	26.789309689203645	20.718978369296266
14	19.92128099414863	24.478885631640214	28.323191740344072	27.276641633867083
15	20.593497454103797	36.28540508556391	23.462730259572414	19.658367200759876
16	19.573687472553484	28.797974953204665	31.536241859480064	20.092095714761786
17	19.19827215706304	31.257902670246096	29.437485596118268	20.106339576572594
18	19.747686490224233	27.22107840236226	34.52977582861183	18.50145927880168
19	23.495671661432585	26.360465686713475	28.71880088240951	21.42506176944443
20	24.463604202860516	27.399735082422694	29.40094519975112	18.735715514965662
21	23.058869700504427	26.61019166408405	27.122640407529513	23.208298227882008
22	23.65373443951091	30.527682213268836	27.56571887785376	18.252864469366493
23	19.360983203400906	30.378490011517535	30.121117115180795	20.139409669900765
24	22.612818020981287	27.248962771921953	30.88759313249109	19.250626074605666
25	20.538116452250186	29.134788421899373	30.466062858642424	19.861032267208024
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	115.0
1	115.0
2	346.5
3	578.0
4	578.0
5	578.0
6	1471.0
7	2364.0
8	2364.0
9	2364.0
10	2788.0
11	3212.0
12	3212.0
13	3212.0
14	4452.5
15	5693.0
16	5693.0
17	5693.0
18	10719.5
19	15746.0
20	15746.0
21	15746.0
22	28173.0
23	40600.0
24	40600.0
25	40600.0
26	62375.0
27	84150.0
28	84150.0
29	84150.0
30	110931.0
31	137712.0
32	137712.0
33	137712.0
34	151666.5
35	165621.0
36	165621.0
37	165621.0
38	169890.0
39	174159.0
40	174159.0
41	174159.0
42	172836.0
43	171513.0
44	171513.0
45	171513.0
46	167512.5
47	163512.0
48	163512.0
49	163512.0
50	145074.0
51	126636.0
52	126636.0
53	126636.0
54	97747.5
55	68859.0
56	68859.0
57	68859.0
58	56975.5
59	45092.0
60	45092.0
61	45092.0
62	38513.0
63	31934.0
64	31934.0
65	31934.0
66	26441.0
67	20948.0
68	20948.0
69	20948.0
70	15619.5
71	10291.0
72	10291.0
73	10291.0
74	8469.5
75	6648.0
76	6648.0
77	6648.0
78	5867.5
79	5087.0
80	5087.0
81	5087.0
82	3528.0
83	1969.0
84	1969.0
85	1969.0
86	1618.0
87	1267.0
88	1267.0
89	1267.0
90	948.5
91	630.0
92	630.0
93	630.0
94	446.5
95	263.0
96	263.0
97	263.0
98	442.5
99	622.0
100	622.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03843696920607429
2	0.0052909188380831
3	0.0017895754893516367
4	0.0038125738686187044
5	0.008558839296899132
6	0.012527028425461459
7	0.02100806009238878
8	0.03532466400720188
9	0.049252229772155924
10	0.05882256825868858
11	0.055788070689787986
12	0.06543621680629245
13	0.06559183206623609
14	0.07049371275446013
15	0.06232391160742004
16	0.07041590512448832
17	0.0657474473261797
18	0.07975282072110555
19	0.0785857062715284
20	0.0815423962104572
21	0.07741859182195124
22	0.08084212754071089
23	0.08496593192921685
24	0.08006405124099279
25	0.08076431991073908
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1285221.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.175963128117985
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.56387610967204	44.72977507832094
2	11.581024675749306	12.548263316384379
3	2.7637957546059626	4.49193890685545
4	0.9913158337275247	2.148219602253677
5	0.4614855943020698	1.2500713270533275
6	0.2868414554053038	0.9323947266992064
7	0.1781922016102683	0.6757613900909243
8	0.14301091508260722	0.6198203249946993
9	0.1113133403763504	0.5427456681507126
>10	0.7133171749549219	7.78333741330625
>50	0.09551445691228994	3.6612124528167014
>100	0.0943063683649013	10.72317270699604
>500	0.010959144702458394	3.9930944102793298
>1k	0.004470177444423819	4.045513832537369
>5k	5.767970896030733E-4	1.8546788432609769
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTAAAGTGTGTATTTCTCATTT	6784	0.5278469617287611	No Hit
GTCCTACAGTGGACATTTCTAAATT	5752	0.44754948759785285	No Hit
CTGTAGGACGTGGAATATGGCAAGA	5684	0.4422585687597697	No Hit
CTTTAGGACGTGAAATATGGCGAGG	5521	0.4295759250743646	No Hit
GTATCAACGCAGAGTACTTTTTTTT	4271	0.3323163876096018	No Hit
GTCCTACAGTGTGCATTTCTCATTT	3893	0.3029051034802575	No Hit
CTGTAGGACCTGGAATATGGCGAGA	3005	0.23381192806528994	No Hit
ATTTAGAAATGTCCACTGTAGGACG	2767	0.21529371213199908	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2762	0.21490467398214003	No Hit
CTGAAGGACCTGGAATATGGCGAGA	2656	0.2066570652051281	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	2321	0.1805915091645717	No Hit
TTTCTAAATTTTCCACCTTTTTCAG	2142	0.16666394339961765	No Hit
GAATATGGCAAGAAAACTGAAAATC	1910	0.14861257324615768	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1558	0.12122428749608044	No Hit
GGAATATGGCGAGAAAACTGAAAAT	1526	0.11873444333698252	No Hit
CTGTAGGACATGGAATATGGCAAGA	1444	0.11235421767929407	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1438	0.11188737189946321	No Hit
GCCATATTCCACGTCCTACAGTGGA	1353	0.10527372335185933	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	7.78076299718103E-5	0.0
13	0.0	0.0	0.0	7.78076299718103E-5	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATCGCC	110	0.0	17.275808	15
CGGAATT	40	2.756895E-4	16.627964	15
TCGCGTA	65	1.910339E-7	16.078537	9
GTCGCGT	65	1.9120307E-7	16.077286	8
CGCATCG	120	0.0	15.836157	13
ATCGCCA	120	0.0	15.835542	16
CCGCATC	125	0.0	15.202711	12
GCATCGC	125	0.0	15.202711	14
TCGCCAG	130	0.0	14.617422	17
GGGTCGC	80	1.2886812E-7	14.249211	6
CGCGTAA	75	9.657997E-7	13.934733	10
ACGATCA	55	1.9580647E-4	13.819571	9
CGAACGA	90	3.630157E-8	13.724134	16
CGCCAGT	140	0.0	13.572791	18
GGTCGCG	85	2.698307E-7	13.411544	7
AGGCCCG	220	0.0	13.387709	10
CCACCTT	740	0.0	13.22533	13
ATCGCTC	65	5.443923E-5	13.15568	16
CGGTCCA	210	0.0	13.120364	10
GTTTTCG	80	1.994631E-6	13.064829	15
>>END_MODULE