FastQCFastQC Report
Thu 2 Feb 2017
SRR4063936_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063936_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1285221
Sequences flagged as poor quality0
Sequence length50
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA31390.2442381504815125No Hit
GTCCTAAAGTGTGTATTTCTCATTTTCCGTGATTTTCAGTTTTCTCGCCA26190.20377818289617117No Hit
CTTTAGGACGTGAAATATGGCGAGGAAAACTGAAAAAGGTGGAAAATTTA24690.1921070384003996No Hit
GTCCTACAGTGGACATTTCTAAATTTTCCACCTTTTTCAGTTTTCCTCGC22110.17203266986767257No Hit
CTGAAGGACCTGGAATATGGCGAGAAAACTGAAAATCACGGAAAATGAGA14260.11095368033980148No Hit
ATTTAGAAATGTCCACTGTAGGACGTGGAATATGGCAAGAAAACTGAAAA14110.10978656589022433No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTACCG250.002353460535.198135
TACCGGA300.005746635629.3317787
AGTCGAG300.005747728529.33063718
ACCGGAG450.001398693724.4431488
GTATCAA37100.024.252081
TAGGACC17450.022.9443424
AGCGGCG2400.022.00140231
GAGTTCG1205.984475E-1021.99883344
GTCCTAC35100.021.6227841
TAGCGGC2500.021.12216630
GGTATCA17000.020.9635931
TCCTACA36900.020.8644772
GGACGTT856.1167357E-620.7047846
AGGACCT24500.020.5621745
TAGAACG755.475215E-520.5322444
ATACCCG755.475215E-520.5322446
TGTAGGA40350.020.4979362
GGACCTG23400.020.3066166
TACCCGG654.934161E-420.3066147
CCTACAG37150.020.1319543
Produced by FastQC (version 0.11.5)