FastQCFastQC Report
Thu 2 Feb 2017
SRR4063935_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063935_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences852277
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT30410.3568088778648256No Hit
TATCAACGCAGAGTACTTTTTTTTT23790.2791346006052023No Hit
GTACTTTTTTTTTTTTTTTTTTTTT22530.26435067472195073No Hit
GAGTACTTTTTTTTTTTTTTTTTTT13380.15699121295071908No Hit
ACGCAGAGTACTTTTTTTTTTTTTT12350.14490594020488645No Hit
GTACATGGGGTGGTATCAACGCAAA11250.1319993382433176No Hit
GGTATCAACGCAGAGTACTTTTTTT10790.12660203196847974No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG8900.10442614314360238No Hit
GAATAGGACCGCGGTTCTATTTTGT8650.10149282451597308No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAC555.921429E-717.2672821
TCGCGTA350.002166876316.2882339
CTAGATT350.002171132516.283454
CGAACGA508.6994485E-515.20145616
CTTATAC508.730016E-515.1952073
CTAAGAC704.4449735E-714.9238643
GTATAGA851.7200364E-814.5248321
CGTCTTA602.5632502E-514.25136615
CGCGTAA400.00527520814.25052910
GTTTTAC400.005287557414.2455081
ACGAACG551.9569203E-413.81950715
TCTTATA551.9647372E-413.8130152
CGACCAT903.6292477E-813.72273210
CGGTCCA1550.013.48437210
GCGAATG1001.00408215E-813.30049314
CGAACGT500.001500148313.2981494
TAACTCC500.001500148313.2981494
ATTGCAC500.001502141713.2958063
GATAACG801.9955405E-613.06298511
ATAACGA801.9968575E-613.06221812
Produced by FastQC (version 0.11.5)