##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063935_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	852277
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.213214717750215	32.0	32.0	32.0	32.0	32.0
2	30.88911586256581	32.0	32.0	32.0	32.0	32.0
3	30.91725342816948	32.0	32.0	32.0	32.0	32.0
4	30.92666703430927	32.0	32.0	32.0	32.0	32.0
5	30.869788812792084	32.0	32.0	32.0	32.0	32.0
6	34.49219678578678	36.0	36.0	36.0	32.0	36.0
7	34.42102743591579	36.0	36.0	36.0	32.0	36.0
8	34.37822445050142	36.0	36.0	36.0	32.0	36.0
9	34.48829312535713	36.0	36.0	36.0	32.0	36.0
10	34.26812996244179	36.0	36.0	36.0	32.0	36.0
11	34.47963396876837	36.0	36.0	36.0	32.0	36.0
12	34.32857392608272	36.0	36.0	36.0	32.0	36.0
13	34.40099286968908	36.0	36.0	36.0	32.0	36.0
14	34.32198569244506	36.0	36.0	36.0	32.0	36.0
15	34.26954851533011	36.0	36.0	36.0	32.0	36.0
16	34.25332139668206	36.0	36.0	36.0	32.0	36.0
17	34.194168093237295	36.0	36.0	36.0	32.0	36.0
18	34.18624226630544	36.0	36.0	36.0	32.0	36.0
19	34.19556083292169	36.0	36.0	36.0	32.0	36.0
20	34.16401005776291	36.0	36.0	36.0	32.0	36.0
21	34.13948986069083	36.0	36.0	36.0	32.0	36.0
22	34.13258482864139	36.0	36.0	36.0	32.0	36.0
23	34.091878579382055	36.0	36.0	36.0	32.0	36.0
24	34.07401349561234	36.0	36.0	36.0	32.0	36.0
25	33.67943286044326	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	2.0
4	7.0
5	7.0
6	41.0
7	17.0
8	45.0
9	51.0
10	100.0
11	19.0
12	43.0
13	37.0
14	99.0
15	192.0
16	313.0
17	371.0
18	526.0
19	656.0
20	996.0
21	1396.0
22	2139.0
23	3066.0
24	4440.0
25	6418.0
26	9064.0
27	12077.0
28	16450.0
29	22246.0
30	29239.0
31	39278.0
32	55438.0
33	79064.0
34	173141.0
35	395298.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.43589493786629	16.739655826084302	10.770077692079258	26.054371543970152
2	16.87906945635759	19.8194625178795	36.99199632025515	26.309471705507764
3	18.742571248546515	24.52576258737033	28.378915898026786	28.35275026605637
4	12.515620471529967	14.945315149246637	35.70830991484863	36.83075446437476
5	14.617494995294521	36.74556851811433	33.306813674756334	15.330122811834809
6	34.14664199202729	35.30440986813463	16.976801223248767	13.572146916589315
7	30.163672382955355	30.38208218123436	21.123900909317097	18.330344526493192
8	28.860397255225095	31.892749070950504	19.48750272904621	19.75935094477819
9	27.983997069886037	13.5160898004799	18.297012128981365	40.2029010006527
10	15.868291944169535	26.322827259674593	31.607679330541576	26.2012014656143
11	37.29574065182197	20.754358139321926	22.640136088762933	19.309765120093168
12	24.82151431935934	23.544025293836565	29.091081997161133	22.543378389642957
13	29.678729052460284	19.194304171250575	25.486461957166224	25.640504819122917
14	24.002944757615214	19.12025953318312	25.557866013528507	31.31892969567315
15	25.446043342839065	26.647076019615696	22.52752219797198	25.379358439573263
16	25.786128077059455	25.27245317046023	23.79177791397501	25.149640838505306
17	24.000544767822067	25.352484740043607	25.338278510200894	25.308691981933435
18	24.892354118841993	24.600797289918802	26.09427747802756	24.412571113211644
19	25.57916984676804	24.50466741032114	25.641754241765984	24.274408501144837
20	26.07176281189416	23.554885974934564	25.04206732886259	25.331283884308682
21	27.04260578057759	23.715632687328643	24.696322998432446	24.545438533661326
22	26.077011503700508	23.532153882102627	25.095844600822648	25.294990013374214
23	24.742332203278373	23.711543587750313	25.50561837335497	26.04050583561634
24	24.81041969521546	24.54703869575326	25.328609775744525	25.313931833286755
25	24.918243764509164	24.114243844357265	25.450290447466408	25.517221943667167
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	201.0
1	201.0
2	386.5
3	572.0
4	572.0
5	572.0
6	1693.0
7	2814.0
8	2814.0
9	2814.0
10	2544.0
11	2274.0
12	2274.0
13	2274.0
14	2097.5
15	1921.0
16	1921.0
17	1921.0
18	3489.5
19	5058.0
20	5058.0
21	5058.0
22	8093.5
23	11129.0
24	11129.0
25	11129.0
26	16808.5
27	22488.0
28	22488.0
29	22488.0
30	27631.5
31	32775.0
32	32775.0
33	32775.0
34	40991.0
35	49207.0
36	49207.0
37	49207.0
38	57259.5
39	65312.0
40	65312.0
41	65312.0
42	76098.5
43	86885.0
44	86885.0
45	86885.0
46	98845.0
47	110805.0
48	110805.0
49	110805.0
50	115769.5
51	120734.0
52	120734.0
53	120734.0
54	112451.0
55	104168.0
56	104168.0
57	104168.0
58	95175.0
59	86182.0
60	86182.0
61	86182.0
62	75582.5
63	64983.0
64	64983.0
65	64983.0
66	53822.5
67	42662.0
68	42662.0
69	42662.0
70	31665.5
71	20669.0
72	20669.0
73	20669.0
74	15687.0
75	10705.0
76	10705.0
77	10705.0
78	8442.0
79	6179.0
80	6179.0
81	6179.0
82	4202.5
83	2226.0
84	2226.0
85	2226.0
86	1699.5
87	1173.0
88	1173.0
89	1173.0
90	836.0
91	499.0
92	499.0
93	499.0
94	355.0
95	211.0
96	211.0
97	211.0
98	328.0
99	445.0
100	445.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03801580941407547
2	0.005162640784627533
3	0.0016426584314723968
4	0.0035199823531551363
5	0.009269286863308525
6	0.014079929412620545
7	0.024522543726980782
8	0.03883713862981167
9	0.05080507863053914
10	0.056789048630902866
11	0.05631971765048218
12	0.0619516894155304
13	0.0664103337295269
14	0.0696956505924717
15	0.05983970000363731
16	0.06699699745505276
17	0.0631250168665821
18	0.07532762235751991
19	0.07356763118094234
20	0.07814360824004403
21	0.0741542949064682
22	0.07485829137709923
23	0.08166359059319915
24	0.07732227902430783
25	0.077439611769413
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	852277.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	51.02028335502173
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.83521548340704	37.67093615542515
2	14.793344934324967	15.095213006356706
3	5.002772580628467	7.657286238731931
4	2.2001148464844347	4.49001931524904
5	1.1605048562335063	2.9604643299956126
6	0.6978885357157302	2.1363882505462657
7	0.4498406780529145	1.6065699201212362
8	0.3147017917284538	1.2844939669054998
9	0.211609448775586	0.9716736633417319
>10	1.1511953534806363	10.560935264447767
>50	0.088867592517785	3.1844205181914673
>100	0.08494190345532761	8.424923615743332
>500	0.007386252467929927	2.490498805451537
>1k	0.0016157427273596717	1.4661769494927008
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3041	0.3568088778648256	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2379	0.2791346006052023	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	2253	0.26435067472195073	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1338	0.15699121295071908	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1235	0.14490594020488645	No Hit
GTACATGGGGTGGTATCAACGCAAA	1125	0.1319993382433176	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1079	0.12660203196847974	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	890	0.10442614314360238	No Hit
GAATAGGACCGCGGTTCTATTTTGT	865	0.10149282451597308	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.173327451051712E-4
2	0.0	0.0	0.0	0.0	1.173327451051712E-4
3	0.0	0.0	0.0	0.0	1.173327451051712E-4
4	0.0	0.0	0.0	0.0	1.173327451051712E-4
5	0.0	0.0	0.0	0.0	1.173327451051712E-4
6	0.0	0.0	0.0	0.0	1.173327451051712E-4
7	0.0	0.0	0.0	0.0	1.173327451051712E-4
8	0.0	0.0	0.0	0.0	1.173327451051712E-4
9	0.0	0.0	0.0	0.0	1.173327451051712E-4
10	0.0	0.0	0.0	0.0	1.173327451051712E-4
11	0.0	0.0	0.0	0.0	1.173327451051712E-4
12	0.0	0.0	0.0	0.0	4.693309804206848E-4
13	0.0	0.0	0.0	0.0	7.039964706310272E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATTAC	55	5.921429E-7	17.267282	1
TCGCGTA	35	0.0021668763	16.288233	9
CTAGATT	35	0.0021711325	16.28345	4
CGAACGA	50	8.6994485E-5	15.201456	16
CTTATAC	50	8.730016E-5	15.195207	3
CTAAGAC	70	4.4449735E-7	14.923864	3
GTATAGA	85	1.7200364E-8	14.524832	1
CGTCTTA	60	2.5632502E-5	14.251366	15
CGCGTAA	40	0.005275208	14.250529	10
GTTTTAC	40	0.0052875574	14.245508	1
ACGAACG	55	1.9569203E-4	13.819507	15
TCTTATA	55	1.9647372E-4	13.813015	2
CGACCAT	90	3.6292477E-8	13.722732	10
CGGTCCA	155	0.0	13.484372	10
GCGAATG	100	1.00408215E-8	13.300493	14
CGAACGT	50	0.0015001483	13.298149	4
TAACTCC	50	0.0015001483	13.298149	4
ATTGCAC	50	0.0015021417	13.295806	3
GATAACG	80	1.9955405E-6	13.062985	11
ATAACGA	80	1.9968575E-6	13.062218	12
>>END_MODULE