FastQCFastQC Report
Thu 2 Feb 2017
SRR4063935_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063935_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences852277
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA9400.11029278039886092No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT8560.10043682981002655No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCGTA2350.025.27133810
CCGTCGT2400.024.744859
ACCGTAT450.001399570424.4393628
ACCGTCG2550.024.1518368
TACCGTC2550.023.289277
GTCGTAG2650.022.41043511
CGGACCG602.872437E-421.99929421
ATACCGT2700.021.9954266
TATGCGA500.002582936821.99542412
CTCGACA500.002582936821.99542412
TCGAACG1451.2732926E-1121.23945444
TACGACG2250.020.5290625
GTATAGG1101.266526E-719.9970131
ATGCGAA550.00448794519.9958413
CGACGGT2350.019.6554857
ACGACGG2350.019.6554856
ACGGTAT2400.019.2459959
TAATCCC1504.656613E-1019.06275
TATAGGA1557.221388E-1018.4488562
GCGAATC600.007419251418.3295215
Produced by FastQC (version 0.11.5)