##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063935_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	852277
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.24370128491089	32.0	32.0	32.0	32.0	32.0
2	31.362011411782788	32.0	32.0	32.0	32.0	32.0
3	31.44444939849368	32.0	32.0	32.0	32.0	32.0
4	31.542618186340825	32.0	32.0	32.0	32.0	32.0
5	31.47249427122872	32.0	32.0	32.0	32.0	32.0
6	35.05247941690319	36.0	36.0	36.0	36.0	36.0
7	35.07317925979464	36.0	36.0	36.0	36.0	36.0
8	35.02149770555817	36.0	36.0	36.0	36.0	36.0
9	35.13135518147269	36.0	36.0	36.0	36.0	36.0
10	34.9786325337889	36.0	36.0	36.0	36.0	36.0
11	35.13848314573783	36.0	36.0	36.0	36.0	36.0
12	35.03673688249243	36.0	36.0	36.0	36.0	36.0
13	35.08485386793261	36.0	36.0	36.0	36.0	36.0
14	35.03038214101753	36.0	36.0	36.0	36.0	36.0
15	34.982132569575384	36.0	36.0	36.0	36.0	36.0
16	34.99588631395661	36.0	36.0	36.0	36.0	36.0
17	34.96608966333716	36.0	36.0	36.0	36.0	36.0
18	34.959775988323045	36.0	36.0	36.0	36.0	36.0
19	34.96085075626821	36.0	36.0	36.0	36.0	36.0
20	34.957390613615054	36.0	36.0	36.0	36.0	36.0
21	34.95210712010297	36.0	36.0	36.0	36.0	36.0
22	34.9287731570839	36.0	36.0	36.0	36.0	36.0
23	34.881021076481005	36.0	36.0	36.0	32.0	36.0
24	34.85074922824387	36.0	36.0	36.0	32.0	36.0
25	34.826867321305166	36.0	36.0	36.0	32.0	36.0
26	34.76583786726616	36.0	36.0	36.0	32.0	36.0
27	34.75328561019481	36.0	36.0	36.0	32.0	36.0
28	34.7311918542915	36.0	36.0	36.0	32.0	36.0
29	34.70749650641751	36.0	36.0	36.0	32.0	36.0
30	34.69564237917954	36.0	36.0	36.0	32.0	36.0
31	34.669847948495615	36.0	36.0	36.0	32.0	36.0
32	34.63288109382278	36.0	36.0	36.0	32.0	36.0
33	34.600113578097265	36.0	36.0	36.0	32.0	36.0
34	34.57709172018018	36.0	36.0	36.0	32.0	36.0
35	34.52548173891822	36.0	36.0	36.0	32.0	36.0
36	34.496453617779196	36.0	36.0	36.0	32.0	36.0
37	34.47871877335655	36.0	36.0	36.0	32.0	36.0
38	34.43865668086784	36.0	36.0	36.0	32.0	36.0
39	34.44386742807796	36.0	36.0	36.0	32.0	36.0
40	34.40975527909353	36.0	36.0	36.0	32.0	36.0
41	34.39035313636294	36.0	36.0	36.0	32.0	36.0
42	34.31864757584682	36.0	36.0	36.0	32.0	36.0
43	34.30371815735964	36.0	36.0	36.0	32.0	36.0
44	34.22576110818431	36.0	36.0	36.0	32.0	36.0
45	34.18505837890733	36.0	36.0	36.0	32.0	36.0
46	34.16498861285709	36.0	36.0	36.0	32.0	36.0
47	34.10398379869456	36.0	36.0	36.0	32.0	36.0
48	34.05348026521894	36.0	36.0	36.0	32.0	36.0
49	34.02685981200948	36.0	36.0	36.0	32.0	36.0
50	33.4048085305599	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	8.0
21	35.0
22	102.0
23	286.0
24	810.0
25	1823.0
26	3574.0
27	6473.0
28	10879.0
29	16234.0
30	22768.0
31	32364.0
32	46186.0
33	74502.0
34	151720.0
35	484510.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.598283016033946	17.703991588749954	11.281377905448538	26.416347489767567
2	16.229836344095105	20.507910582956697	36.615368730317414	26.646884342630788
3	18.106212695257728	25.02384804833204	28.680548792578886	28.189390463831337
4	12.179021609171665	15.657350837814466	35.78226327825343	36.381364274760436
5	14.18940086380367	37.057670217546644	33.216430808293545	15.53649811035614
6	34.23343404417588	35.68777683259511	16.646856941714823	13.431932181514183
7	29.980663540918666	30.508778846289232	21.180579999906136	18.329977612885965
8	28.74370656488442	32.239635705293	19.33702305705774	19.67963467276484
9	28.075760117990917	13.311172880862731	18.17935417104224	40.43371283010411
10	15.825093602636715	26.505474184914927	31.572160563575284	26.097271648873072
11	37.50224398875014	20.647395154392292	22.569422851960102	19.280938004897468
12	24.823267552685337	23.507263483585735	28.953028182152046	22.716440781576882
13	29.81047108255473	19.160337498283997	25.39884614166151	25.63034527749976
14	23.92889618961016	19.02155994250682	25.527793259217972	31.521750608665045
15	25.467561405752598	26.823515883417954	22.48929586818752	25.21962684264193
16	25.95973107113943	25.187675267227522	23.709387870041184	25.143205791591868
17	24.150481592252284	25.333782326637937	25.259276033496153	25.256460047613626
18	25.059517174620865	24.431081517115956	26.18908216244757	24.32031914581561
19	25.65929346831308	24.417559569904583	25.72687720879152	24.196269752990816
20	26.109800884696167	23.46169641076185	25.13428842972297	25.29421427481901
21	27.20418361635947	23.57742846515863	24.687278901108442	24.53110901737346
22	26.190546031395893	23.53917799025434	25.16916448525538	25.10111149309438
23	24.770407534692605	23.490884941526815	25.687956060924282	26.050751462856297
24	24.981256049983866	24.414185562171834	25.382417646886275	25.222140740958025
25	24.969991516663363	23.938028228508003	25.72152362134046	25.370456633488175
26	25.04517570415429	24.70313108698611	25.791102850410923	24.460590358448677
27	25.49514970952234	24.37243096535272	24.979788011738794	25.15263131338614
28	24.883600863606496	24.072092368346944	25.96850652398385	25.075800244062705
29	24.854356628841437	24.313202769600107	25.627718529591565	25.204722071966895
30	24.822964665683365	24.63475278416298	25.60831511644143	24.93396743371222
31	25.16061276336093	24.44787345767744	24.937074999559965	25.454438779401674
32	24.985655466000814	24.577437602743824	24.668961009855195	25.767945921400166
33	24.823876730138316	24.180633715227096	25.19231581565843	25.80317373897616
34	25.466503176903355	24.126043567284057	25.425904522760472	24.981548733052115
35	26.00187738339689	24.128131416837782	25.34115576415371	24.528835435611615
36	25.03740297228953	24.917596118304868	24.8252474463304	25.2197534630752
37	25.885053101426013	24.610055653532996	24.773038485773323	24.73185275926767
38	25.0422434065083	24.56653571210649	25.182585390352546	25.208635491032666
39	25.426934729500445	24.520497795216826	24.69673964315133	25.3558278321314
40	25.62475506128455	24.777589779572757	24.967673956355735	24.62998120278696
41	24.639954755272488	25.185801416009777	25.317452308818734	24.856791519898998
42	25.918646796337736	25.292311374828547	24.575627831445605	24.213413997388116
43	25.07606255844761	24.358557668502183	25.059518291974626	25.505861481075588
44	24.936345865473296	24.975418357265724	24.540340881090554	25.547894896170426
45	25.044734053849826	25.229889036079406	24.583489291400852	25.141887618669912
46	24.804257810620825	24.99433864190766	24.567243440744647	25.634160106726867
47	25.059284178017975	24.94100916279359	24.918832597439017	25.080874061749412
48	25.82263628470185	25.334639663952313	24.04242836693028	24.800295684415556
49	25.053973126076528	25.438707648695964	24.295298706052968	25.212020519174537
50	25.18438992338109	25.535921714949488	24.102925129360415	25.176763232309007
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	92.0
1	70.0
2	48.0
3	132.0
4	216.0
5	250.0
6	284.0
7	269.0
8	254.0
9	314.5
10	375.0
11	589.0
12	803.0
13	1395.5
14	1988.0
15	2536.5
16	3085.0
17	3417.5
18	3750.0
19	3736.5
20	3723.0
21	3943.5
22	4164.0
23	4514.5
24	4865.0
25	4942.0
26	5019.0
27	5834.0
28	6649.0
29	7617.5
30	8586.0
31	9760.0
32	10934.0
33	12691.0
34	14448.0
35	16687.0
36	18926.0
37	22903.5
38	26881.0
39	30040.5
40	33200.0
41	37170.5
42	41141.0
43	42801.5
44	44462.0
45	49580.0
46	54698.0
47	58072.5
48	61447.0
49	64254.5
50	67062.0
51	65972.5
52	64883.0
53	64886.5
54	64890.0
55	64994.5
56	65099.0
57	63068.5
58	61038.0
59	55978.5
60	50919.0
61	44977.0
62	39035.0
63	33767.5
64	28500.0
65	24164.5
66	19829.0
67	16801.5
68	13774.0
69	12157.5
70	10541.0
71	8251.5
72	5962.0
73	5004.5
74	4047.0
75	3172.5
76	2298.0
77	2013.0
78	1728.0
79	1388.0
80	1048.0
81	884.0
82	720.0
83	592.5
84	465.0
85	326.0
86	187.0
87	130.5
88	74.0
89	62.0
90	50.0
91	40.0
92	30.0
93	21.5
94	13.0
95	16.5
96	20.0
97	19.0
98	18.0
99	13.5
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009973283333939553
2	1.173327451051712E-4
3	7.039964706310272E-4
4	0.0
5	0.0
6	2.346654902103424E-4
7	1.173327451051712E-4
8	0.0
9	9.386619608413696E-4
10	4.693309804206848E-4
11	0.0
12	0.0
13	7.039964706310272E-4
14	2.346654902103424E-4
15	0.004223978823786163
16	8.213292157361984E-4
17	0.0
18	2.346654902103424E-4
19	1.173327451051712E-4
20	8.213292157361984E-4
21	0.0
22	0.0
23	7.039964706310272E-4
24	2.346654902103424E-4
25	0.0021119894118930816
26	0.005749304510153389
27	0.0070399647063102725
28	0.004341311568891335
29	0.002815985882524109
30	0.005397306274837875
31	0.008447957647572326
32	0.004223978823786163
33	0.00387198058847065
34	0.0037546478433654784
35	0.0031679841178396226
36	0.008447957647572326
37	0.004693309804206848
38	0.008565290392677498
39	0.004575977059101677
40	0.0026986531374189374
41	0.0026986531374189374
42	0.0021119894118930816
43	0.0021119894118930816
44	0.0017599911765775681
45	0.0021119894118930816
46	9.386619608413696E-4
47	0.0030506513727344515
48	0.0019946566667879105
49	1.173327451051712E-4
50	8.213292157361984E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	852277.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.27949324375357
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.89097002673701	40.65043901584423
2	14.4317443071701	15.666955352332371
3	4.800995373649163	7.817867878418457
4	2.108865406317998	4.578725822968936
5	1.0667863057154816	2.895231003680616
6	0.6758233337022882	2.2010008845398623
7	0.3915768757931878	1.4878216068818526
8	0.2615660490624876	1.135813807430609
9	0.20018096211398903	0.9779149062534921
>10	1.0126602969582414	9.808688840947964
>50	0.07941684969050308	3.0133398520867045
>100	0.07353973706477321	7.647883967536185
>500	0.005874476024782193	2.118317061078768
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	940	0.11029278039886092	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	856	0.10043682981002655	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.346654902103424E-4
2	0.0	0.0	0.0	0.0	2.346654902103424E-4
3	0.0	0.0	0.0	0.0	2.346654902103424E-4
4	0.0	0.0	0.0	0.0	2.346654902103424E-4
5	0.0	0.0	0.0	0.0	2.346654902103424E-4
6	0.0	0.0	0.0	0.0	2.346654902103424E-4
7	0.0	0.0	0.0	0.0	2.346654902103424E-4
8	0.0	0.0	0.0	0.0	3.519982353155136E-4
9	0.0	0.0	0.0	0.0	3.519982353155136E-4
10	0.0	0.0	0.0	0.0	3.519982353155136E-4
11	0.0	0.0	0.0	0.0	3.519982353155136E-4
12	0.0	0.0	0.0	0.0	7.039964706310272E-4
13	0.0	0.0	0.0	0.0	9.386619608413696E-4
14	0.0	0.0	0.0	0.0	9.386619608413696E-4
15	0.0	0.0	0.0	0.0	0.0010559947059465408
16	0.0	0.0	0.0	0.0	0.0010559947059465408
17	0.0	0.0	0.0	0.0	0.0010559947059465408
18	0.0	0.0	0.0	0.0	0.0010559947059465408
19	0.0	0.0	0.0	1.173327451051712E-4	0.0010559947059465408
20	0.0	0.0	0.0	3.519982353155136E-4	0.0010559947059465408
21	0.0	0.0	0.0	9.386619608413696E-4	0.001173327451051712
22	0.0	0.0	0.0	0.0014079929412620545	0.0014079929412620545
23	0.0	0.0	0.0	0.002698653137418938	0.0014079929412620545
24	0.0	0.0	0.0	0.004223978823786163	0.0014079929412620545
25	0.0	0.0	0.0	0.005045308039522362	0.0014079929412620545
26	0.0	0.0	0.0	0.005514639019943046	0.0014079929412620545
27	0.0	0.0	0.0	0.006570633725889587	0.0017599911765775681
28	0.0	0.0	0.0	0.008565290392677498	0.0018773239216827392
29	0.0	0.0	0.0	0.012906601961568833	0.0018773239216827392
30	0.0	0.0	0.0	0.020063899412984275	0.0018773239216827392
31	0.0	0.0	0.0	0.03602115274728756	0.0018773239216827392
32	0.0	0.0	0.0	0.0542077282385891	0.0018773239216827392
33	0.0	0.0	0.0	0.07028231431799756	0.0018773239216827392
34	0.0	0.0	0.0	0.09034621373098183	0.0021119894118930816
35	0.0	0.0	0.0	0.11322609902649021	0.0021119894118930816
36	0.0	0.0	0.0	0.14654859863635883	0.0021119894118930816
37	0.0	0.0	0.0	0.18644173197211705	0.0021119894118930816
38	0.0	0.0	0.0	0.2566067135450094	0.0021119894118930816
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCGTA	235	0.0	25.271338	10
CCGTCGT	240	0.0	24.74485	9
ACCGTAT	45	0.0013995704	24.439362	8
ACCGTCG	255	0.0	24.151836	8
TACCGTC	255	0.0	23.28927	7
GTCGTAG	265	0.0	22.410435	11
CGGACCG	60	2.872437E-4	21.999294	21
ATACCGT	270	0.0	21.995426	6
TATGCGA	50	0.0025829368	21.995424	12
CTCGACA	50	0.0025829368	21.995424	12
TCGAACG	145	1.2732926E-11	21.239454	44
TACGACG	225	0.0	20.529062	5
GTATAGG	110	1.266526E-7	19.997013	1
ATGCGAA	55	0.004487945	19.99584	13
CGACGGT	235	0.0	19.655485	7
ACGACGG	235	0.0	19.655485	6
ACGGTAT	240	0.0	19.245995	9
TAATCCC	150	4.656613E-10	19.0627	5
TATAGGA	155	7.221388E-10	18.448856	2
GCGAATC	60	0.0074192514	18.32952	15
>>END_MODULE