FastQCFastQC Report
Thu 2 Feb 2017
SRR4063934_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063934_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2151236
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTAAAGTGTGTATTTCTCATTT44980.2090891004055343No Hit
CTGTAGGACGTGGAATATGGCAAGA42830.1990948459397295No Hit
GTCCTACAGTGGACATTTCTAAATT39130.1818954312776469No Hit
CTTTAGGACGTGAAATATGGCGAGG35290.16404522795267465No Hit
GTCCTACAGTGTGCATTTCTCATTT25880.12030293282559422No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCCAA350.002169995816.28635210
GGTATCA9300.015.8295341
CCGGTTT902.2882887E-914.77870112
TCCAACG1550.013.48690918
GCCGGTT1001.0073563E-813.30052111
CCAACGA1102.737579E-912.95806819
GTATCAA28600.012.7521851
TAGGACC9150.012.6645124
CCAACGT550.00306286112.09419619
TATACGT550.003073017812.0888524
GACGTGG9800.011.9236597
GTCGAGG802.8595912E-511.87822819
GTCCTAA14750.011.7836121
AGGACCT16200.011.2581285
GGCGAGG9400.011.12004519
TGGCGAG21550.011.06745818
GGACGAG700.001492475710.8565586
TGTAGGA24450.010.8358742
GGACGTG19950.010.8089426
AGGACGT20400.010.7562725
Produced by FastQC (version 0.11.5)