##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063934_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2151236
Sequences flagged as poor quality	0
Sequence length	25
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.34261373461582	32.0	32.0	32.0	32.0	32.0
2	31.06701310316488	32.0	32.0	32.0	32.0	32.0
3	31.078923000544805	32.0	32.0	32.0	32.0	32.0
4	31.098997971398767	32.0	32.0	32.0	32.0	32.0
5	31.09163615707435	32.0	32.0	32.0	32.0	32.0
6	34.74171034698192	36.0	36.0	36.0	32.0	36.0
7	34.687605636945456	36.0	36.0	36.0	32.0	36.0
8	34.665924147792246	36.0	36.0	36.0	32.0	36.0
9	34.742910122366865	36.0	36.0	36.0	32.0	36.0
10	34.559467673467715	36.0	36.0	36.0	32.0	36.0
11	34.7167939733251	36.0	36.0	36.0	32.0	36.0
12	34.60369341160152	36.0	36.0	36.0	32.0	36.0
13	34.62796318023685	36.0	36.0	36.0	32.0	36.0
14	34.58521473236781	36.0	36.0	36.0	32.0	36.0
15	34.55678038113903	36.0	36.0	36.0	32.0	36.0
16	34.550799633327074	36.0	36.0	36.0	32.0	36.0
17	34.51836107242534	36.0	36.0	36.0	32.0	36.0
18	34.50284208706065	36.0	36.0	36.0	32.0	36.0
19	34.49176194522591	36.0	36.0	36.0	32.0	36.0
20	34.45770385025167	36.0	36.0	36.0	32.0	36.0
21	34.45104023919272	36.0	36.0	36.0	32.0	36.0
22	34.42986636519657	36.0	36.0	36.0	32.0	36.0
23	34.39862153664219	36.0	36.0	36.0	32.0	36.0
24	34.38133380066157	36.0	36.0	36.0	32.0	36.0
25	33.993751034289126	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	16.0
5	30.0
6	79.0
7	27.0
8	125.0
9	119.0
10	191.0
11	45.0
12	139.0
13	121.0
14	167.0
15	313.0
16	528.0
17	731.0
18	907.0
19	1118.0
20	1513.0
21	2236.0
22	3184.0
23	4892.0
24	7732.0
25	11837.0
26	17487.0
27	24006.0
28	34038.0
29	47602.0
30	63521.0
31	88471.0
32	126515.0
33	182585.0
34	421627.0
35	1109331.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.08231523574973	19.198145847129116	12.099333338293613	25.620205578827537
2	14.12984206839132	21.127542337227894	42.19125748853737	22.55135810584342
3	18.14812886527012	25.570749086094885	29.8850692262208	26.396052822414195
4	11.218222126562482	17.636716852389636	39.431920611507465	31.71314040954042
5	12.176841463221875	38.90649799119865	36.21421139786726	12.702449147712217
6	30.18883363141992	38.87628716919711	19.071745807997523	11.863133391385444
7	27.767078767139207	32.22366873259344	22.486551328128996	17.52270117213835
8	24.7137613126012	37.82428836751643	20.727717279600164	16.734233040282206
9	26.822141299141006	15.25041973034941	20.265046344741627	37.66239262576796
10	13.765554463062307	30.21958804219112	35.381060508105485	20.63379698664109
11	33.93114860996321	22.754064903576708	25.131171307099226	18.183615179360864
12	23.088920218934067	25.70042574647502	32.44673750192453	18.76391653266638
13	28.953685954611146	22.660237610259077	26.013629959303625	22.37244647582615
14	20.62795642504513	22.63031295949747	28.38730895022577	28.354421665231627
15	22.21442359124631	32.109490918393455	23.657387381101884	22.018698109258356
16	21.24230039641155	28.987955080180072	28.390221166199797	21.379523357208576
17	21.007035292749578	29.34789231932089	28.187964416535845	21.457107971393686
18	20.74172149691581	28.383778092991296	30.371018923821346	20.50348148627154
19	23.15545610599132	27.465772861123643	27.813277757360638	21.565493275524396
20	23.80383915931334	27.722971266087022	28.223929404353886	20.249260170245748
21	23.442870879549016	27.09408623784839	26.897725311486298	22.56531757111629
22	23.156293396417333	29.016685159210333	27.1330974661726	20.69392397819973
23	21.794441743451127	28.836171702533015	27.73108031720259	21.638306236813275
24	22.528202296378	27.70759025455919	28.5801413220107	21.184066127052105
25	22.013856065145465	28.33431339345196	28.177162614304162	21.474667927098416
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	52.0
1	52.0
2	222.5
3	393.0
4	393.0
5	393.0
6	892.0
7	1391.0
8	1391.0
9	1391.0
10	2495.5
11	3600.0
12	3600.0
13	3600.0
14	6292.5
15	8985.0
16	8985.0
17	8985.0
18	15929.5
19	22874.0
20	22874.0
21	22874.0
22	37483.5
23	52093.0
24	52093.0
25	52093.0
26	76473.0
27	100853.0
28	100853.0
29	100853.0
30	133892.5
31	166932.0
32	166932.0
33	166932.0
34	194325.0
35	221718.0
36	221718.0
37	221718.0
38	243746.0
39	265774.0
40	265774.0
41	265774.0
42	281887.5
43	298001.0
44	298001.0
45	298001.0
46	305226.5
47	312452.0
48	312452.0
49	312452.0
50	294628.5
51	276805.0
52	276805.0
53	276805.0
54	235360.0
55	193915.0
56	193915.0
57	193915.0
58	156908.5
59	119902.0
60	119902.0
61	119902.0
62	91408.5
63	62915.0
64	62915.0
65	62915.0
66	45117.5
67	27320.0
68	27320.0
69	27320.0
70	18392.5
71	9465.0
72	9465.0
73	9465.0
74	6264.0
75	3063.0
76	3063.0
77	3063.0
78	2078.0
79	1093.0
80	1093.0
81	1093.0
82	729.5
83	366.0
84	366.0
85	366.0
86	292.5
87	219.0
88	219.0
89	219.0
90	188.5
91	158.0
92	158.0
93	158.0
94	139.5
95	121.0
96	121.0
97	121.0
98	448.5
99	776.0
100	776.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03811762168353449
2	0.005345764016593252
3	0.0013015773257792265
4	0.004323096117766717
5	0.009389950707407276
6	0.013713046825173993
7	0.021661965493325698
8	0.035235557605023345
9	0.048623210098752534
10	0.05624673443545943
11	0.05508461182315655
12	0.0634518946317373
13	0.06568317004735882
14	0.06828632469891727
15	0.05977958717686019
16	0.06740311151356708
17	0.06326595501376883
18	0.08009349043991454
19	0.07558445470417936
20	0.08158100738366222
21	0.07544499999070302
22	0.08000052063093031
23	0.08464901108014183
24	0.07795518483327724
25	0.07939621687253282
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2151236.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	70.93942713205365
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.07265073263972	57.512473990505306
2	13.39824774844988	19.00928039696724
3	3.295672764746249	7.013794138374309
4	1.085168880378485	3.0792503486232707
5	0.4244650615419975	1.5055654151680598
6	0.2049745539965396	0.8724466462297631
7	0.10257457490650364	0.5093607107526906
8	0.06874535847871799	0.39014050787743365
9	0.04931809278134991	0.3148737524238989
>10	0.2555583688705345	3.3547617161285044
>50	0.02273104028360204	1.118054538963632
>100	0.01600916578692757	2.328907134104195
>500	0.0030279360749307987	1.5601954975895524
>1k	8.557210646542788E-4	1.4308952062921547
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTCCTAAAGTGTGTATTTCTCATTT	4498	0.2090891004055343	No Hit
CTGTAGGACGTGGAATATGGCAAGA	4283	0.1990948459397295	No Hit
GTCCTACAGTGGACATTTCTAAATT	3913	0.1818954312776469	No Hit
CTTTAGGACGTGAAATATGGCGAGG	3529	0.16404522795267465	No Hit
GTCCTACAGTGTGCATTTCTCATTT	2588	0.12030293282559422	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	4.648490449211523E-5	0.0
6	0.0	0.0	0.0	4.648490449211523E-5	0.0
7	0.0	0.0	0.0	4.648490449211523E-5	0.0
8	0.0	0.0	0.0	4.648490449211523E-5	0.0
9	0.0	0.0	0.0	9.296980898423046E-5	0.0
10	0.0	0.0	0.0	1.394547134763457E-4	0.0
11	0.0	0.0	0.0	1.394547134763457E-4	0.0
12	0.0	0.0	0.0	1.394547134763457E-4	0.0
13	0.0	0.0	0.0	1.394547134763457E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCCAA	35	0.0021699958	16.286352	10
GGTATCA	930	0.0	15.829534	1
CCGGTTT	90	2.2882887E-9	14.778701	12
TCCAACG	155	0.0	13.486909	18
GCCGGTT	100	1.0073563E-8	13.300521	11
CCAACGA	110	2.737579E-9	12.958068	19
GTATCAA	2860	0.0	12.752185	1
TAGGACC	915	0.0	12.664512	4
CCAACGT	55	0.003062861	12.094196	19
TATACGT	55	0.0030730178	12.088852	4
GACGTGG	980	0.0	11.923659	7
GTCGAGG	80	2.8595912E-5	11.878228	19
GTCCTAA	1475	0.0	11.783612	1
AGGACCT	1620	0.0	11.258128	5
GGCGAGG	940	0.0	11.120045	19
TGGCGAG	2155	0.0	11.067458	18
GGACGAG	70	0.0014924757	10.856558	6
TGTAGGA	2445	0.0	10.835874	2
GGACGTG	1995	0.0	10.808942	6
AGGACGT	2040	0.0	10.756272	5
>>END_MODULE