FastQCFastQC Report
Thu 2 Feb 2017
SRR4063934_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063934_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2151236
Sequences flagged as poor quality0
Sequence length50
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA23750.11040164816877368No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATCCG300.0057479429.3313035
GGTATCA11350.029.0722031
GTATCAA29850.027.267161
TGCGGCG500.002579336522.0025725
TAGGACC11050.020.903534
GTCCTAC24900.020.4960981
TTACGCG550.004485920519.99908310
TAATACG550.00448652419.9986174
TATCAAC41650.019.171852
ATCAACG41400.019.1291123
TAGGACG31000.019.0894
AGGACGT30650.019.0198925
GTACCGT1051.7952007E-618.8558396
ATACCGT1051.7952007E-618.8558396
TCAACGC41900.018.8483374
GACGTGA16500.018.6658087
GTAGGAC30100.018.56353
GGACGTG30050.018.5211816
TTAGGAC19950.018.4147663
CAACGCA43050.018.3959395
Produced by FastQC (version 0.11.5)