##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063934_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2151236
Sequences flagged as poor quality	0
Sequence length	50
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.372481215450094	32.0	32.0	32.0	32.0	32.0
2	31.43041860586193	32.0	32.0	32.0	32.0	32.0
3	31.507893601631807	32.0	32.0	32.0	32.0	32.0
4	31.576564821339918	32.0	32.0	32.0	32.0	32.0
5	31.5553779315705	32.0	32.0	32.0	32.0	32.0
6	35.1416739028168	36.0	36.0	36.0	36.0	36.0
7	35.16316480386159	36.0	36.0	36.0	36.0	36.0
8	35.10462496908754	36.0	36.0	36.0	36.0	36.0
9	35.21028655154525	36.0	36.0	36.0	36.0	36.0
10	35.08992272349477	36.0	36.0	36.0	36.0	36.0
11	35.21199208269107	36.0	36.0	36.0	36.0	36.0
12	35.14322975257015	36.0	36.0	36.0	36.0	36.0
13	35.17665518799425	36.0	36.0	36.0	36.0	36.0
14	35.12982490066176	36.0	36.0	36.0	36.0	36.0
15	35.11130577956114	36.0	36.0	36.0	36.0	36.0
16	35.13133984369916	36.0	36.0	36.0	36.0	36.0
17	35.116032364649904	36.0	36.0	36.0	36.0	36.0
18	35.108439985199205	36.0	36.0	36.0	36.0	36.0
19	35.09556645574916	36.0	36.0	36.0	36.0	36.0
20	35.08967077531243	36.0	36.0	36.0	36.0	36.0
21	35.08714153165901	36.0	36.0	36.0	36.0	36.0
22	35.06301958501996	36.0	36.0	36.0	36.0	36.0
23	35.02576332861666	36.0	36.0	36.0	36.0	36.0
24	35.01671132316491	36.0	36.0	36.0	36.0	36.0
25	34.988739961584876	36.0	36.0	36.0	36.0	36.0
26	34.9455782629149	36.0	36.0	36.0	36.0	36.0
27	34.917615268617666	36.0	36.0	36.0	36.0	36.0
28	34.89171573923084	36.0	36.0	36.0	36.0	36.0
29	34.87737840013834	36.0	36.0	36.0	36.0	36.0
30	34.86025010738013	36.0	36.0	36.0	36.0	36.0
31	34.84553159207079	36.0	36.0	36.0	32.0	36.0
32	34.818444838223236	36.0	36.0	36.0	32.0	36.0
33	34.82360233837664	36.0	36.0	36.0	32.0	36.0
34	34.816605430552485	36.0	36.0	36.0	32.0	36.0
35	34.789629775626665	36.0	36.0	36.0	32.0	36.0
36	34.761362770054056	36.0	36.0	36.0	32.0	36.0
37	34.733081819010096	36.0	36.0	36.0	32.0	36.0
38	34.71107400582735	36.0	36.0	36.0	32.0	36.0
39	34.670011565444234	36.0	36.0	36.0	32.0	36.0
40	34.68724863287896	36.0	36.0	36.0	32.0	36.0
41	34.66805734005939	36.0	36.0	36.0	32.0	36.0
42	34.647481261935	36.0	36.0	36.0	32.0	36.0
43	34.63886342549121	36.0	36.0	36.0	32.0	36.0
44	34.59698703443044	36.0	36.0	36.0	32.0	36.0
45	34.571054035912375	36.0	36.0	36.0	32.0	36.0
46	34.5334254354241	36.0	36.0	36.0	32.0	36.0
47	34.523069528401344	36.0	36.0	36.0	32.0	36.0
48	34.495593695903196	36.0	36.0	36.0	32.0	36.0
49	34.46747869596827	36.0	36.0	36.0	32.0	36.0
50	33.94841849057937	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	3.0
18	1.0
19	2.0
20	13.0
21	43.0
22	155.0
23	489.0
24	1271.0
25	2930.0
26	6379.0
27	11876.0
28	20838.0
29	32528.0
30	48213.0
31	70124.0
32	101527.0
33	162783.0
34	343990.0
35	1348071.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.363254099492394	20.056446615364802	12.51025657594701	26.070042709195796
2	13.638354050259851	21.95893492994673	41.42323890629503	22.979472113498385
3	17.53059182941092	26.258072133683648	30.12023830110058	26.09109773580485
4	10.969972611094274	18.372089347705227	39.35486390149663	31.303074139703874
5	11.85862453027004	39.5371776969147	35.79063384956369	12.813563923251564
6	30.296471699498984	39.25663131021544	18.751748996157556	11.695147994128021
7	27.784551664508996	32.30088762966389	22.43520582363154	17.479354882195576
8	24.852270973524057	38.02632533111198	20.42718697530164	16.69421672006233
9	26.765628348417142	15.142225071274073	20.152491419917133	37.93965516039165
10	13.716931401397353	30.525704138601068	35.18336192189512	20.574002538106463
11	34.033815010361494	22.770000846026047	25.02744936162221	18.168734781990246
12	23.171269685803445	25.798553666664652	32.21282864292245	18.81734800460945
13	28.96097388187015	22.619938583858694	25.992015715689565	22.427071818581588
14	20.64298099226954	22.719885832756145	28.332814250452067	28.30431892452225
15	22.172708631526685	32.26429224567894	23.608778378438686	21.95422074435569
16	21.47724821682249	29.003642590981098	28.13334411793144	21.385765074264974
17	21.061287557478582	29.516426835549424	27.984656262725245	21.43762934424675
18	20.890261952220868	28.49994793681016	30.14714359027757	20.4626465206914
19	23.310506146234072	27.464955030503397	27.788582935577498	21.435955887685033
20	23.966400461133684	27.799760136108816	28.012895008413828	20.22094439434367
21	23.571286460434838	27.063929759449916	26.857955147645352	22.50682863246989
22	23.328413678654357	28.971358312783124	27.094808331028457	20.60541967753407
23	21.929219435369458	28.858476059077393	27.614293709778487	21.598010795774663
24	22.714954170319622	27.757241684509204	28.35513330538028	21.172670839790893
25	22.181325265920606	28.21617269568791	28.062677400490703	21.539824637900786
26	21.03075798794672	29.007919610100057	28.523380422069287	21.437941979883927
27	21.828231668057434	28.757539764631503	27.718534048937865	21.6956945183732
28	21.115917337046085	28.99623223910987	27.492242503410974	22.395607920433072
29	22.129570446056476	28.26567682463386	27.19840460025521	22.40634812905445
30	21.573390034418573	28.32145759226168	28.014182089324663	22.090970283995084
31	22.72477927448807	27.581995715656447	27.780360450153594	21.91286455970188
32	21.656614716352774	28.784171130154085	27.91157134901295	21.647642804480192
33	21.352024154578142	28.72879327056056	28.561161613931183	21.35802096093011
34	21.684939626018338	28.768928608783572	28.59534905342429	20.9507827117738
35	21.59162064177472	28.86322042068789	28.211302483136087	21.333856454401307
36	21.1379007049469	28.511020490134275	28.884134696738943	21.466944108179874
37	21.54093890345203	28.288804831233623	27.41509383967508	22.75516242563927
38	21.574960844940385	29.123275329453392	27.662509187318086	21.63925463828814
39	22.509977291057975	27.79393204052744	27.92623429027002	21.76985637814456
40	22.813176514777165	27.654634107338676	28.34290400062105	21.189285377263108
41	22.68564617634562	27.26717002252736	28.58320293869081	21.46398086243621
42	21.744969500431857	28.613113373230853	28.461150123327776	21.180767003009514
43	21.29156120202957	28.478636292851185	28.642870590532237	21.58693191458701
44	20.931169835738803	28.54981945028096	28.4548030687874	22.06420764519284
45	21.32835877094344	28.553405500436728	28.07646483771234	22.041770890907493
46	21.430610136936124	27.557871758470302	28.43588434852377	22.575633756069806
47	22.345652727702088	27.593357716759296	28.136360002008193	21.924629553530423
48	21.026009814112015	28.090989722888004	28.317794632547226	22.565205830452754
49	21.97989057422963	27.630983204957165	28.099877744360203	22.289248476453004
50	21.13932212072374	28.85874873152725	27.93145621834048	22.07047292940853
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	2.0
2	4.0
3	14.0
4	24.0
5	36.0
6	48.0
7	69.0
8	90.0
9	137.0
10	184.0
11	315.5
12	447.0
13	782.0
14	1117.0
15	1556.5
16	1996.0
17	2574.0
18	3152.0
19	3974.5
20	4797.0
21	6449.0
22	8101.0
23	10740.5
24	13380.0
25	17747.0
26	22114.0
27	29028.0
28	35942.0
29	45740.0
30	55538.0
31	67646.0
32	79754.0
33	95880.5
34	112007.0
35	129287.5
36	146568.0
37	153720.5
38	160873.0
39	161889.0
40	162905.0
41	164419.0
42	165933.0
43	167355.5
44	168778.0
45	170488.0
46	172198.0
47	170978.0
48	169758.0
49	165376.0
50	160994.0
51	151208.5
52	141423.0
53	129195.0
54	116967.0
55	103293.5
56	89620.0
57	76592.5
58	63565.0
59	52531.5
60	41498.0
61	33048.0
62	24598.0
63	18957.5
64	13317.0
65	10024.0
66	6731.0
67	5015.0
68	3299.0
69	2451.5
70	1604.0
71	1200.5
72	797.0
73	602.0
74	407.0
75	321.0
76	235.0
77	203.5
78	172.0
79	144.0
80	116.0
81	85.0
82	54.0
83	55.5
84	57.0
85	40.5
86	24.0
87	20.5
88	17.0
89	14.0
90	11.0
91	10.5
92	10.0
93	7.5
94	5.0
95	4.5
96	4.0
97	2.5
98	1.0
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.008227828095104394
2	7.437584718738437E-4
3	3.7187923593692184E-4
4	0.0
5	0.0
6	9.296980898423046E-5
7	4.648490449211523E-5
8	0.0
9	0.0015340018482398025
10	0.001208607516794996
11	9.296980898423046E-5
12	1.394547134763457E-4
13	4.648490449211523E-4
14	2.789094269526914E-4
15	0.004044186690814025
16	9.296980898423046E-4
17	0.0
18	1.8593961796846092E-4
19	0.0
20	7.437584718738437E-4
21	0.0
22	4.648490449211523E-5
23	0.0011156377078107656
24	4.648490449211523E-4
25	0.001394547134763457
26	0.005578188539053828
27	0.006554371533388248
28	0.004230126308782486
29	0.0023707301290978766
30	0.0033469131234322965
31	0.006879765864833054
32	0.003625822550384988
33	0.004230126308782486
34	0.002835579174019029
35	0.002091820702145185
36	0.007065705482801514
37	0.0031144886009717206
38	0.008041888477135935
39	0.004694975353703638
40	0.002091820702145185
41	0.003439882932416527
42	0.0026961244605426832
43	0.0019058810841767243
44	0.0013015773257792265
45	0.0011621226123028807
46	6.507886628896132E-4
47	0.002231275415621531
48	0.002231275415621531
49	2.789094269526914E-4
50	0.0012550924212871113
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2151236.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	76.84506164399193
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.12511508661945	63.10909531351202
2	13.030409435265167	20.026452325988114
3	3.0793772999819833	7.0990481532667475
4	0.9334725587343914	2.8693102527567667
5	0.35068934635306687	1.3474372219196329
6	0.1524612759156252	0.7029537687634727
7	0.07816793698648272	0.42047739554169583
8	0.049082875537083424	0.3017421277049247
9	0.035215034927583905	0.2435491376824962
>10	0.15117016957552815	2.0234302726202325
>50	0.008806898555443999	0.46495767664271404
>100	0.005423599885636164	0.8596648801025045
>500	3.0424083120466136E-4	0.15463138750431943
>1k	3.0424083120414496E-4	0.37725008599430604
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	2375	0.11040164816877368	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	4.648490449211523E-5	0.0
6	0.0	0.0	0.0	4.648490449211523E-5	0.0
7	0.0	0.0	0.0	4.648490449211523E-5	0.0
8	0.0	0.0	0.0	4.648490449211523E-5	0.0
9	0.0	0.0	0.0	4.648490449211523E-5	0.0
10	0.0	0.0	0.0	4.648490449211523E-5	0.0
11	0.0	0.0	0.0	4.648490449211523E-5	0.0
12	0.0	0.0	0.0	4.648490449211523E-5	0.0
13	0.0	0.0	0.0	4.648490449211523E-5	0.0
14	0.0	0.0	0.0	4.648490449211523E-5	0.0
15	0.0	0.0	0.0	4.648490449211523E-5	0.0
16	0.0	0.0	0.0	4.648490449211523E-5	0.0
17	0.0	0.0	0.0	4.648490449211523E-5	0.0
18	0.0	0.0	0.0	4.648490449211523E-5	0.0
19	0.0	0.0	0.0	4.648490449211523E-5	0.0
20	0.0	0.0	0.0	9.296980898423046E-5	0.0
21	0.0	0.0	0.0	1.394547134763457E-4	0.0
22	0.0	0.0	0.0	1.8593961796846092E-4	0.0
23	0.0	0.0	0.0	2.3242452246057614E-4	0.0
24	0.0	0.0	0.0	3.253943314448066E-4	0.0
25	0.0	0.0	0.0	4.1836414042903706E-4	0.0
26	0.0	0.0	0.0	6.507886628896132E-4	0.0
27	0.0	0.0	0.0	9.296980898423046E-4	0.0
28	0.0	0.0	0.0	0.0025101848425742226	0.0
29	0.0	0.0	0.0	0.005206309303116906	0.0
30	0.0	0.0	0.0	0.011388801600568231	0.0
31	0.0	0.0	0.0	0.033887495374752004	0.0
32	0.0	0.0	0.0	0.06247571163740287	0.0
33	0.0	0.0	0.0	0.09324871841118315	0.0
34	0.0	0.0	0.0	0.13108743066776496	0.0
35	0.0	0.0	0.0	0.17194766171633424	0.0
36	0.0	0.0	0.0	0.23079755080335213	0.0
37	0.0	0.0	0.0	0.3205134164731345	0.0
38	0.0	0.0	0.0	0.4429081700008739	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATCCG	30	0.00574794	29.331303	5
GGTATCA	1135	0.0	29.072203	1
GTATCAA	2985	0.0	27.26716	1
TGCGGCG	50	0.0025793365	22.00257	25
TAGGACC	1105	0.0	20.90353	4
GTCCTAC	2490	0.0	20.496098	1
TTACGCG	55	0.0044859205	19.999083	10
TAATACG	55	0.004486524	19.998617	4
TATCAAC	4165	0.0	19.17185	2
ATCAACG	4140	0.0	19.129112	3
TAGGACG	3100	0.0	19.089	4
AGGACGT	3065	0.0	19.019892	5
GTACCGT	105	1.7952007E-6	18.855839	6
ATACCGT	105	1.7952007E-6	18.855839	6
TCAACGC	4190	0.0	18.848337	4
GACGTGA	1650	0.0	18.665808	7
GTAGGAC	3010	0.0	18.5635	3
GGACGTG	3005	0.0	18.521181	6
TTAGGAC	1995	0.0	18.414766	3
CAACGCA	4305	0.0	18.395939	5
>>END_MODULE