FastQCFastQC Report
Thu 2 Feb 2017
SRR4063933_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063933_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1204514
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT43810.36371515814677124No Hit
GTACTTTTTTTTTTTTTTTTTTTTT33450.27770536498538No Hit
TATCAACGCAGAGTACTTTTTTTTT33390.27720723877015957No Hit
GAGTACTTTTTTTTTTTTTTTTTTT21360.17733293261846686No Hit
GTACATGGAAGCAGTGGTATCAACG21230.17625365915215596No Hit
TCCATGTACTCTGCGTTGATACCAC18340.1522605797857061No Hit
ACGCAGAGTACTTTTTTTTTTTTTT16980.14096971890737675No Hit
GGTATCAACGCAGAGTACTTTTTTT15510.12876562663447663No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG14300.11872008129419832No Hit
GTACATGGGGTGGTATCAACGCAAA13540.1124104825680731No Hit
CTATTGGAGCTGGAATTACCGCGGC13280.11025193563545131No Hit
GTCCTATTCCATTATTCCTAGCTGC12770.1060178628060778No Hit
GTATCTGATCGTCTTCGAACCTCCG12770.1060178628060778No Hit
GAATAGGACCGCGGTTCTATTTTGT12650.10502161037563697No Hit
GATTAAGAGGGACGGCCGGGGGCAT12610.1046895262321567No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAT551.1282607E-515.544917
ACGCGTA551.1291497E-515.5436196
TACGCGT551.1295942E-515.5429735
ATACGCG551.1304841E-515.5416814
CTATTAC400.005292522314.2441761
GCGCCGA1750.013.57490219
CGCATCG1900.013.50233313
CCGTCGT1850.013.352559
CGTCGTA1850.013.3525510
CGTCTTA1001.002627E-813.30340315
TTATACG802.0112748E-613.0549932
CGTATGC604.0877398E-412.6678039
ATAATAC604.106405E-412.6609643
ATACCGT2050.012.5107186
CGTTATT1001.4521902E-712.3429012
ATCCCCG2350.012.129251512
GTATTAC550.00307716312.0859671
GAGTTAG550.00307716312.0859671
GTTAGAC550.0030781212.08546453
GCATCGC2150.011.9327914