##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063933_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1204514
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.237620318236235	32.0	32.0	32.0	32.0	32.0
2	31.016962858048974	32.0	32.0	32.0	32.0	32.0
3	31.03990987236346	32.0	32.0	32.0	32.0	32.0
4	31.043612610563265	32.0	32.0	32.0	32.0	32.0
5	31.027065687904003	32.0	32.0	32.0	32.0	32.0
6	34.597048270090674	36.0	36.0	36.0	32.0	36.0
7	34.54030671291492	36.0	36.0	36.0	32.0	36.0
8	34.49558162047099	36.0	36.0	36.0	32.0	36.0
9	34.57536400573177	36.0	36.0	36.0	32.0	36.0
10	34.41032897915674	36.0	36.0	36.0	32.0	36.0
11	34.560686716800305	36.0	36.0	36.0	32.0	36.0
12	34.45005537503093	36.0	36.0	36.0	32.0	36.0
13	34.501147350715726	36.0	36.0	36.0	32.0	36.0
14	34.44380472124027	36.0	36.0	36.0	32.0	36.0
15	34.38764265089488	36.0	36.0	36.0	32.0	36.0
16	34.38352563772609	36.0	36.0	36.0	32.0	36.0
17	34.329613437452785	36.0	36.0	36.0	32.0	36.0
18	34.31643301779805	36.0	36.0	36.0	32.0	36.0
19	34.31307232626603	36.0	36.0	36.0	32.0	36.0
20	34.26756600587457	36.0	36.0	36.0	32.0	36.0
21	34.23391591961571	36.0	36.0	36.0	32.0	36.0
22	34.22634108030293	36.0	36.0	36.0	32.0	36.0
23	34.18091778094734	36.0	36.0	36.0	32.0	36.0
24	34.15312067771732	36.0	36.0	36.0	32.0	36.0
25	33.68194558137141	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	6.0
4	16.0
5	17.0
6	49.0
7	18.0
8	69.0
9	83.0
10	116.0
11	43.0
12	85.0
13	63.0
14	128.0
15	293.0
16	482.0
17	605.0
18	824.0
19	1138.0
20	1497.0
21	1880.0
22	2738.0
23	3779.0
24	5232.0
25	7236.0
26	10334.0
27	13664.0
28	19288.0
29	26918.0
30	36983.0
31	53585.0
32	79428.0
33	118609.0
34	271557.0
35	547751.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.90922983392744	17.306312351958223	11.326504170916206	25.457953643198135
2	16.20936326229688	20.185030204607575	38.125222092702735	25.48038444039281
3	18.686612070855002	24.962058567623753	28.776708443753694	27.57462091776755
4	11.929060443583941	15.775757143023192	36.640840474834405	35.65434193855846
5	13.88909415629974	37.293453703988156	34.04658453940622	14.77086760030588
6	33.25209847491586	36.17298291909091	17.495912654951688	13.079005951041536
7	29.317858708794493	30.92713218978781	21.527842366102867	18.227166735314828
8	27.916798166204345	33.038220686676745	19.912296729398868	19.132684417720046
9	27.57006667558222	13.96316437811127	18.72348696599105	39.74328198031546
10	15.456536150233182	26.931454366322143	32.303606466951656	25.30840301649302
11	36.79773390871673	21.118093426424718	23.100507959977904	18.983664704880653
12	24.609401934485362	23.89337817170115	29.719596820219802	21.777623073593684
13	29.595632827895134	19.73850801024833	25.688206774400435	24.977652387456097
14	23.341256146688078	20.212894108733344	25.80508217599612	30.64076756858246
15	24.91287736871793	27.59139465160501	23.2323140020087	24.26341397766836
16	25.06548629336364	25.760268242491613	24.908967198283275	24.265278265861472
17	23.286675467111728	25.830524695383588	26.334459557690405	24.548340279814276
18	24.146030400844147	24.890888470693803	27.645990935413785	23.31709019304827
19	25.010115377617225	25.248148698958882	26.078897452918838	23.662838470505054
20	25.273669788267867	24.521311554411163	25.987558462513782	24.21746019480719
21	26.544468427690514	24.450448564245466	24.75395017356005	24.25113283450397
22	25.197017219533475	24.865172506906507	25.47859501900588	24.459215254554138
23	23.8196014500882	24.61527212418977	26.045252523238222	25.519873902483813
24	24.21485728718954	25.58419902107977	25.930169470683488	24.2707742210472
25	24.5267216861794	24.788276240826274	26.037556508828295	24.64744556416603
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	206.0
1	206.0
2	567.5
3	929.0
4	929.0
5	929.0
6	2623.5
7	4318.0
8	4318.0
9	4318.0
10	4126.0
11	3934.0
12	3934.0
13	3934.0
14	3749.5
15	3565.0
16	3565.0
17	3565.0
18	6313.0
19	9061.0
20	9061.0
21	9061.0
22	14651.0
23	20241.0
24	20241.0
25	20241.0
26	31052.5
27	41864.0
28	41864.0
29	41864.0
30	51824.5
31	61785.0
32	61785.0
33	61785.0
34	72022.0
35	82259.0
36	82259.0
37	82259.0
38	91135.0
39	100011.0
40	100011.0
41	100011.0
42	112838.0
43	125665.0
44	125665.0
45	125665.0
46	144670.0
47	163675.0
48	163675.0
49	163675.0
50	160701.0
51	157727.0
52	157727.0
53	157727.0
54	143639.0
55	129551.0
56	129551.0
57	129551.0
58	117819.5
59	106088.0
60	106088.0
61	106088.0
62	93547.0
63	81006.0
64	81006.0
65	81006.0
66	67395.5
67	53785.0
68	53785.0
69	53785.0
70	40417.5
71	27050.0
72	27050.0
73	27050.0
74	21000.0
75	14950.0
76	14950.0
77	14950.0
78	12339.0
79	9728.0
80	9728.0
81	9728.0
82	6611.5
83	3495.0
84	3495.0
85	3495.0
86	2737.0
87	1979.0
88	1979.0
89	1979.0
90	1377.0
91	775.0
92	775.0
93	775.0
94	509.0
95	243.0
96	243.0
97	243.0
98	433.5
99	624.0
100	624.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.038355718571971766
2	0.0051473042239442626
3	0.002158546932621788
4	0.004317093865243576
5	0.010211587412018457
6	0.015524933707702858
7	0.02540443697624104
8	0.03769155028501121
9	0.05188814741879297
10	0.060771398256890326
11	0.05637128335577668
12	0.06500547108626384
13	0.06691495491127541
14	0.06782818630584617
15	0.062348797938421636
16	0.06865839666454686
17	0.06533755522974412
18	0.07712654232329387
19	0.07480195331893194
20	0.08011529961461636
21	0.0743868481395816
22	0.07795675268199456
23	0.08310405690593883
24	0.07828883682547483
25	0.0781227947537347
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1204514.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.48613013252631
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.39658017324584	36.07202267694925
2	14.84121335972307	14.391860045682417
3	4.942407372474324	7.189146210892426
4	2.1195222295481653	4.110697225626185
5	1.0690745331825724	2.591764346863001
6	0.6006539758281683	1.7474032101974333
7	0.38552681300511266	1.308489225746082
8	0.2571531382920425	0.9974688421772407
9	0.19812240114481378	0.8645569671668419
>10	0.9588320109360905	8.551946200899957
>50	0.10363976870632842	3.5605777548547346
>100	0.11046442342197736	11.218474405919398
>500	0.01286464323333906	4.207364137484944
>1k	0.003945157258223978	3.1882287495401243
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4381	0.36371515814677124	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	3345	0.27770536498538	No Hit
TATCAACGCAGAGTACTTTTTTTTT	3339	0.27720723877015957	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	2136	0.17733293261846686	No Hit
GTACATGGAAGCAGTGGTATCAACG	2123	0.17625365915215596	No Hit
TCCATGTACTCTGCGTTGATACCAC	1834	0.1522605797857061	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1698	0.14096971890737675	No Hit
GGTATCAACGCAGAGTACTTTTTTT	1551	0.12876562663447663	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1430	0.11872008129419832	No Hit
GTACATGGGGTGGTATCAACGCAAA	1354	0.1124104825680731	No Hit
CTATTGGAGCTGGAATTACCGCGGC	1328	0.11025193563545131	No Hit
GTCCTATTCCATTATTCCTAGCTGC	1277	0.1060178628060778	No Hit
GTATCTGATCGTCTTCGAACCTCCG	1277	0.1060178628060778	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1265	0.10502161037563697	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1261	0.1046895262321567	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.302103587006876E-5
2	0.0	0.0	0.0	0.0	8.302103587006876E-5
3	0.0	0.0	0.0	0.0	8.302103587006876E-5
4	0.0	0.0	0.0	0.0	8.302103587006876E-5
5	0.0	0.0	0.0	0.0	8.302103587006876E-5
6	0.0	0.0	0.0	0.0	8.302103587006876E-5
7	0.0	0.0	0.0	8.302103587006876E-5	1.6604207174013752E-4
8	0.0	0.0	0.0	8.302103587006876E-5	1.6604207174013752E-4
9	0.0	0.0	0.0	1.6604207174013752E-4	4.151051793503438E-4
10	0.0	0.0	0.0	1.6604207174013752E-4	4.151051793503438E-4
11	0.0	0.0	0.0	1.6604207174013752E-4	4.981262152204126E-4
12	0.0	0.0	0.0	1.6604207174013752E-4	6.641682869605501E-4
13	0.0	0.0	0.0	1.6604207174013752E-4	9.132313945707563E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGTAT	55	1.1282607E-5	15.54491	7
ACGCGTA	55	1.1291497E-5	15.543619	6
TACGCGT	55	1.1295942E-5	15.542973	5
ATACGCG	55	1.1304841E-5	15.541681	4
CTATTAC	40	0.0052925223	14.244176	1
GCGCCGA	175	0.0	13.574902	19
CGCATCG	190	0.0	13.502333	13
CCGTCGT	185	0.0	13.35255	9
CGTCGTA	185	0.0	13.35255	10
CGTCTTA	100	1.002627E-8	13.303403	15
TTATACG	80	2.0112748E-6	13.054993	2
CGTATGC	60	4.0877398E-4	12.667803	9
ATAATAC	60	4.106405E-4	12.660964	3
ATACCGT	205	0.0	12.510718	6
CGTTATT	100	1.4521902E-7	12.342901	2
ATCCCCG	235	0.0	12.1292515	12
GTATTAC	55	0.003077163	12.085967	1
GAGTTAG	55	0.003077163	12.085967	1
GTTAGAC	55	0.00307812	12.0854645	3
GCATCGC	215	0.0	11.93279	14
>>END_MODULE