FastQCFastQC Report
Thu 2 Feb 2017
SRR4063933_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063933_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1204514
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA13590.11282558774742345No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA13390.11116516703002206No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA13350.1108330828865418No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT12940.10742922041586897No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA12920.10726317834412882No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTAGAC602.8727137E-422.0000653
GATATAC4900.021.1012251
CCGTCGT3100.019.162149
CGTCGTA3050.018.75415210
ATACACT5450.018.5688614
ACCGTCG3200.018.5617838
GTTATAT1801.4551915E-1118.3326241
CGTCTTA1951.8189894E-1218.05208415
GTCATAC1102.797442E-617.9993061
AAACGCT2700.017.92597629
ATATACA5950.017.3774782
GTAAACG2800.017.28648227
GTATTAC1408.3757186E-817.2850461
GTCGTAG3200.017.1875511
TAAACGC2950.017.15259228
ATCCCGT3350.017.07467520
AACGCTT2850.016.98320830
CCCGTTT3500.016.97147622
AATCCCG3250.016.92312619
ACTGTTC5900.016.7790138
Produced by FastQC (version 0.11.5)