##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063933_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1204514
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.174346665958222	32.0	32.0	32.0	32.0	32.0
2	31.24035337073708	32.0	32.0	32.0	32.0	32.0
3	31.372688901913968	32.0	32.0	32.0	32.0	32.0
4	31.492122964116646	32.0	32.0	32.0	32.0	32.0
5	31.396943497543408	32.0	32.0	32.0	32.0	32.0
6	34.98198609563691	36.0	36.0	36.0	36.0	36.0
7	35.011318257820164	36.0	36.0	36.0	36.0	36.0
8	34.94997733525721	36.0	36.0	36.0	36.0	36.0
9	35.094907987785945	36.0	36.0	36.0	36.0	36.0
10	34.904011078327024	36.0	36.0	36.0	32.0	36.0
11	35.09799637032031	36.0	36.0	36.0	36.0	36.0
12	34.98139830670295	36.0	36.0	36.0	36.0	36.0
13	35.04105805328954	36.0	36.0	36.0	36.0	36.0
14	34.97838464310087	36.0	36.0	36.0	36.0	36.0
15	34.93452546006107	36.0	36.0	36.0	36.0	36.0
16	34.943774003456994	36.0	36.0	36.0	36.0	36.0
17	34.90012071258616	36.0	36.0	36.0	32.0	36.0
18	34.90090111032333	36.0	36.0	36.0	32.0	36.0
19	34.90789895343682	36.0	36.0	36.0	32.0	36.0
20	34.88224711377369	36.0	36.0	36.0	32.0	36.0
21	34.87213017034256	36.0	36.0	36.0	32.0	36.0
22	34.847516923838164	36.0	36.0	36.0	32.0	36.0
23	34.78855538416324	36.0	36.0	36.0	32.0	36.0
24	34.7594440579354	36.0	36.0	36.0	32.0	36.0
25	34.72378403239813	36.0	36.0	36.0	32.0	36.0
26	34.66449954089367	36.0	36.0	36.0	32.0	36.0
27	34.653436987863984	36.0	36.0	36.0	32.0	36.0
28	34.62291015297456	36.0	36.0	36.0	32.0	36.0
29	34.57930003304237	36.0	36.0	36.0	32.0	36.0
30	34.55790883294009	36.0	36.0	36.0	32.0	36.0
31	34.543930581130645	36.0	36.0	36.0	32.0	36.0
32	34.49309431023633	36.0	36.0	36.0	32.0	36.0
33	34.45707646403446	36.0	36.0	36.0	32.0	36.0
34	34.440558598737745	36.0	36.0	36.0	32.0	36.0
35	34.381748157348106	36.0	36.0	36.0	32.0	36.0
36	34.349495315122944	36.0	36.0	36.0	32.0	36.0
37	34.332804766071625	36.0	36.0	36.0	32.0	36.0
38	34.261861630499936	36.0	36.0	36.0	32.0	36.0
39	34.25576373541528	36.0	36.0	36.0	32.0	36.0
40	34.20833381762271	36.0	36.0	36.0	32.0	36.0
41	34.19438877422761	36.0	36.0	36.0	32.0	36.0
42	34.10088965342038	36.0	36.0	36.0	32.0	36.0
43	34.097694173749744	36.0	36.0	36.0	32.0	36.0
44	34.015480102348334	36.0	36.0	36.0	32.0	36.0
45	33.948702132146245	36.0	36.0	36.0	32.0	36.0
46	33.93888821549604	36.0	36.0	36.0	32.0	36.0
47	33.88098602423882	36.0	36.0	36.0	32.0	36.0
48	33.837437339873176	36.0	36.0	36.0	32.0	36.0
49	33.81710714860932	36.0	36.0	36.0	32.0	36.0
50	33.14409380048717	36.0	36.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	5.0
19	3.0
20	13.0
21	37.0
22	130.0
23	438.0
24	1097.0
25	2588.0
26	5308.0
27	9695.0
28	16018.0
29	24609.0
30	35290.0
31	51004.0
32	75092.0
33	119282.0
34	239438.0
35	624466.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.705632392525565	18.378547125352657	11.890145181190045	26.025675300931727
2	15.416310366871175	21.075042963528738	37.577023021809694	25.931623647790385
3	17.939538749048367	25.575110812971612	29.130673379233162	27.35467705874686
4	11.671761390901226	16.47876238881408	36.7540767479664	35.09539947231829
5	13.569456228819258	37.8627396609753	33.524641473656594	15.043162636548848
6	33.226374476655714	36.635054997330876	17.162786952071084	12.975783573942332
7	29.27090684178629	31.145694099675385	21.325020132668058	18.25837892587027
8	27.96364342797178	33.244694540702724	19.64551678104198	19.146145250283517
9	27.60261087154691	13.748088421604946	18.42114002301366	40.22816068383448
10	15.361190466109258	27.159168553336936	32.17493024093755	25.304710739616255
11	37.145634791820434	21.02193998736419	22.899545293409037	18.932879927406347
12	24.585724343136473	23.97942071145825	29.514110278685475	21.9207446667198
13	29.712371472098496	19.624576070668034	25.668718685269056	24.994333771964417
14	23.329613984750672	20.190417364044528	25.694887224037615	30.78508142716718
15	25.032545813213602	27.64658899951513	23.023589072577163	24.297276114694103
16	25.201680023445277	25.857300953834223	24.71915895881053	24.221860063909965
17	23.45917108476946	25.98151619657389	25.983010575219552	24.576302143437104
18	24.383006879970377	24.92853946539301	27.275799058705154	23.41265459593146
19	25.217079433762855	25.387189677487914	25.784943790560998	23.61078709818823
20	25.544933395876313	24.475614464032944	25.774986405203798	24.204465734886945
21	26.721565710319684	24.383942403326156	24.61498994615256	24.27950194020161
22	25.294455103431844	24.886904499992944	25.305662952579176	24.51297744399604
23	23.921860017185484	24.57914246931312	25.854106043561465	25.644891469939935
24	24.30959362660937	25.67006611828232	25.590863655534047	24.429476599574265
25	24.804086719076253	24.66327988595975	25.895920836007363	24.636712558956635
26	23.953428708778322	25.695991391800128	26.44970704057428	23.900872858847276
27	24.864147243216493	25.204640539209866	25.52470921905733	24.40650299851631
28	23.75495659451238	25.31698957869777	26.24653785609437	24.681515970695482
29	23.94607784125392	25.16627548720316	26.1131594874788	24.77448718406412
30	24.124417994513706	25.45672071042861	26.348243522386873	24.070617772670815
31	24.481943796361026	25.270714360439918	25.396253422849107	24.85108842034995
32	24.032352872803596	25.612552595834842	25.265013267268206	25.09008126409336
33	23.803166427138052	25.02596623020153	26.067106203749596	25.103761138910823
34	24.374951223197666	25.372156623765218	26.252214674386824	24.00067747865029
35	25.196001185596494	25.239838366998573	25.917322079576106	23.646838367828824
36	24.087607604017215	25.90857311759392	25.533287501992668	24.470531776396196
37	24.96193295883446	25.743780999523437	25.247871649726765	24.04641439191534
38	24.246067495028583	25.617223728292863	25.6029425806532	24.533766196025358
39	24.600457805737268	25.189693808164947	25.38704677107471	24.822801615023067
40	25.177006432672798	25.75410385927158	25.56647039874601	23.50241930930961
41	23.834830546468957	25.726051508559856	26.462564136625545	23.97655380834565
42	25.46119487247609	26.340744553666312	25.177337938363443	23.020722635494156
43	24.272307329563002	25.339147125925287	25.719473437764634	24.669072106747077
44	23.992375202471415	25.873667798264993	25.327794064048632	24.80616293521496
45	24.220627084900382	26.19772286121807	25.25342591992986	24.328224133951686
46	23.94437175382899	25.662268699937567	25.435285795884756	24.958073750348696
47	24.59976736984293	25.448095330211117	25.714349760520456	24.237787539425497
48	25.055293387043747	26.312346199700453	24.518050883199777	24.114309530056026
49	24.081347669465316	26.215329233737567	25.306992309729527	24.396330787067594
50	24.177996440004783	26.7125835204102	24.504606075902284	24.604813963682734
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	62.0
1	48.0
2	34.0
3	129.5
4	225.0
5	269.5
6	314.0
7	304.0
8	294.0
9	407.5
10	521.0
11	750.5
12	980.0
13	1678.5
14	2377.0
15	3196.5
16	4016.0
17	4541.5
18	5067.0
19	5288.5
20	5510.0
21	6229.0
22	6948.0
23	7721.5
24	8495.0
25	9109.0
26	9723.0
27	12407.5
28	15092.0
29	17171.5
30	19251.0
31	21127.5
32	23004.0
33	26816.0
34	30628.0
35	35327.5
36	40027.0
37	45542.5
38	51058.0
39	53493.5
40	55929.0
41	59795.5
42	63662.0
43	64020.0
44	64378.0
45	71410.0
46	78442.0
47	83466.5
48	88491.0
49	89412.0
50	90333.0
51	85737.0
52	81141.0
53	79248.5
54	77356.0
55	78447.5
56	79539.0
57	76959.5
58	74380.0
59	67742.5
60	61105.0
61	54189.0
62	47273.0
63	41215.0
64	35157.0
65	30046.0
66	24935.0
67	21746.5
68	18558.0
69	16787.0
70	15016.0
71	11758.0
72	8500.0
73	7351.5
74	6203.0
75	4837.5
76	3472.0
77	3068.5
78	2665.0
79	2192.5
80	1720.0
81	1426.0
82	1132.0
83	929.0
84	726.0
85	549.0
86	372.0
87	266.5
88	161.0
89	120.5
90	80.0
91	73.0
92	66.0
93	52.0
94	38.0
95	32.5
96	27.0
97	22.5
98	18.0
99	15.5
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.007305851156566051
2	3.3208414348027504E-4
3	5.811472510904813E-4
4	0.0
5	0.0
6	8.302103587006876E-5
7	3.3208414348027504E-4
8	0.0
9	9.962524304408251E-4
10	9.132313945707564E-4
11	8.302103587006876E-5
12	1.6604207174013752E-4
13	7.471893228306188E-4
14	1.6604207174013752E-4
15	0.004815220080463988
16	5.811472510904813E-4
17	0.0
18	2.490631076102063E-4
19	8.302103587006876E-5
20	7.471893228306188E-4
21	0.0
22	8.302103587006876E-5
23	7.471893228306188E-4
24	4.981262152204126E-4
25	0.0022415679684918565
26	0.006724703905475569
27	0.007056788048955844
28	0.004815220080463988
29	0.0030717783271925437
30	0.004815220080463988
31	0.008966271873967426
32	0.003818967650023163
33	0.00539636733155447
34	0.0039850097217633005
35	0.004732199044593919
36	0.008136061515266737
37	0.004981262152204125
38	0.01070971362723887
39	0.006060535618515019
40	0.003154799363062613
41	0.004483135936983713
42	0.0028227152195823375
43	0.0018264627891415128
44	0.0019094838250115814
45	0.0016604207174013752
46	0.0014943786456612376
47	0.0022415679684918565
48	0.0024906310761020627
49	4.151051793503438E-4
50	0.0014943786456612376
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1204514.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.39871572025559
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.99711313371034	40.581482397871014
2	14.132657239554817	15.093314926135992
3	4.626874958544225	7.412075417534174
4	1.9435320661954687	4.151284651838911
5	1.0038164818801938	2.680125547561378
6	0.5667848032814723	1.8159348350992994
7	0.34413749867331844	1.2863550322235457
8	0.23380651129675767	0.9987973944224231
9	0.1616381561267221	0.776814295370141
>10	0.8030888600127137	7.895804820287032
>50	0.08814103612788235	3.2776077141280378
>100	0.08730106061724167	9.51400753240032
>500	0.009230752742984846	3.316033437127093
>1k	0.0018774412358613245	1.2003619980006568
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1359	0.11282558774742345	No Hit
GTACATGGGGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1339	0.11116516703002206	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1335	0.1108330828865418	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1294	0.10742922041586897	No Hit
CTATTGGAGCTGGAATTACCGCGGCTGCTGGCACCAGACTTGCCCTCCAA	1292	0.10726317834412882	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	2.490631076102063E-4
7	0.0	0.0	0.0	0.0	2.490631076102063E-4
8	0.0	0.0	0.0	0.0	2.490631076102063E-4
9	0.0	0.0	0.0	2.490631076102063E-4	2.490631076102063E-4
10	0.0	0.0	0.0	2.490631076102063E-4	2.490631076102063E-4
11	0.0	0.0	0.0	2.490631076102063E-4	2.490631076102063E-4
12	0.0	0.0	0.0	2.490631076102063E-4	9.132313945707563E-4
13	0.0	0.0	0.0	2.490631076102063E-4	9.962524304408251E-4
14	0.0	0.0	0.0	2.490631076102063E-4	9.962524304408251E-4
15	0.0	0.0	0.0	2.490631076102063E-4	0.001079273466310894
16	0.0	0.0	0.0	5.811472510904813E-4	0.0011622945021809625
17	0.0	0.0	0.0	5.811472510904813E-4	0.0011622945021809625
18	0.0	0.0	0.0	5.811472510904813E-4	0.0011622945021809625
19	0.0	0.0	0.0	5.811472510904813E-4	0.0011622945021809625
20	0.0	0.0	0.0	6.641682869605501E-4	0.0011622945021809625
21	0.0	0.0	0.0	7.471893228306188E-4	0.0011622945021809625
22	0.0	0.0	0.0	0.0014943786456612376	0.0011622945021809625
23	0.0	0.0	0.0	0.0033208414348027504	0.0013283365739211002
24	0.0	0.0	0.0	0.005147304223944263	0.0013283365739211002
25	0.0	0.0	0.0	0.005728451475034744	0.0013283365739211002
26	0.0	0.0	0.0	0.006890745977215707	0.0013283365739211002
27	0.0	0.0	0.0	0.008219082551136807	0.0014943786456612376
28	0.0	0.0	0.0	0.011539923985939558	0.0014943786456612376
29	0.0	0.0	0.0	0.01743441753271444	0.0014943786456612376
30	0.0	0.0	0.0	0.02872527841104379	0.0014943786456612376
31	0.0	0.0	0.0	0.05130700016770249	0.0015773996815313064
32	0.0	0.0	0.0	0.079700194435266	0.0016604207174013752
33	0.0	0.0	0.0	0.10419140001693629	0.0016604207174013752
34	0.0	0.0	0.0	0.13416199396603112	0.0016604207174013752
35	0.0	0.0	0.0	0.1679515555651491	0.0016604207174013752
36	0.0	0.0	0.0	0.21427729358064745	0.0016604207174013752
37	0.0	0.0	0.0	0.2878339313615284	0.0016604207174013752
38	0.0	0.0	0.0	0.3818967650023163	0.001743441753271444
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTAGAC	60	2.8727137E-4	22.000065	3
GATATAC	490	0.0	21.101225	1
CCGTCGT	310	0.0	19.16214	9
CGTCGTA	305	0.0	18.754152	10
ATACACT	545	0.0	18.568861	4
ACCGTCG	320	0.0	18.561783	8
GTTATAT	180	1.4551915E-11	18.332624	1
CGTCTTA	195	1.8189894E-12	18.052084	15
GTCATAC	110	2.797442E-6	17.999306	1
AAACGCT	270	0.0	17.925976	29
ATATACA	595	0.0	17.377478	2
GTAAACG	280	0.0	17.286482	27
GTATTAC	140	8.3757186E-8	17.285046	1
GTCGTAG	320	0.0	17.18755	11
TAAACGC	295	0.0	17.152592	28
ATCCCGT	335	0.0	17.074675	20
AACGCTT	285	0.0	16.983208	30
CCCGTTT	350	0.0	16.971476	22
AATCCCG	325	0.0	16.923126	19
ACTGTTC	590	0.0	16.779013	8
>>END_MODULE