FastQCFastQC Report
Thu 2 Feb 2017
SRR4063932_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063932_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1158551
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT32480.28035019606387634No Hit
TATCAACGCAGAGTACTTTTTTTTT24840.21440575339367884No Hit
GTACTTTTTTTTTTTTTTTTTTTTT19030.16425690366673543No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG13090.11298596263781223No Hit
GAGTACTTTTTTTTTTTTTTTTTTT12870.11108703889600026No Hit
GAATAGGACCGCGGTTCTATTTTGT12360.10668498840361797No Hit
ACGCAGAGTACTTTTTTTTTTTTTT12030.10383660279090001No Hit
GATTAAGAGGGACGGCCGGGGGCAT11930.10297345563553092No Hit
GTATCTGATCGTCTTCGAACCTCCG11830.10211030848016187No Hit
CTATTGGAGCTGGAATTACCGCGGC11690.10090190246264516No Hit
GTCCTATTCCATTATTCCTAGCTGC11640.10047032888496062No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAACCG1600.013.0606895
GTATAGA957.3894626E-812.9987581
GGTTCTA2200.012.95554713
CGGTCCA2150.012.81494310
GTTCTAG604.09392E-412.6654581
CGAGCCG2300.012.39279715
GACGACC853.9355564E-612.29559915
GCGACGA853.937457E-612.29506713
CGTTATT1251.4169927E-912.1556882
GCGTAAC550.003065857612.09184411
TACGCCT550.003070820612.0892325
CGAACGT550.003072807612.0881884
TCGAACG550.003075790412.0866223
AAGCTCG1201.0000804E-811.8748937
CTTATAG802.875295E-511.87079053
CGCGGTT2650.011.83110310
TAGAACC1701.8189894E-1211.7326544
ATTCGAA658.027136E-411.6911921
GAACCGC1302.6157068E-911.6911926
GTCTTAC658.027136E-411.6911921
Produced by FastQC (version 0.11.5)