##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063932_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1158551
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.244342286183343	32.0	32.0	32.0	32.0	32.0
2	30.864151858657927	32.0	32.0	32.0	32.0	32.0
3	30.88111442655524	32.0	32.0	32.0	32.0	32.0
4	30.91286788410696	32.0	32.0	32.0	32.0	32.0
5	30.82489247344312	32.0	32.0	32.0	32.0	32.0
6	34.47256357294586	36.0	36.0	36.0	32.0	36.0
7	34.39776410360873	36.0	36.0	36.0	32.0	36.0
8	34.35977699730094	36.0	36.0	36.0	32.0	36.0
9	34.48713436007564	36.0	36.0	36.0	32.0	36.0
10	34.199992922193324	36.0	36.0	36.0	32.0	36.0
11	34.47803851535237	36.0	36.0	36.0	32.0	36.0
12	34.29465427072265	36.0	36.0	36.0	32.0	36.0
13	34.3834669341272	36.0	36.0	36.0	32.0	36.0
14	34.2779981200655	36.0	36.0	36.0	32.0	36.0
15	34.2203174482608	36.0	36.0	36.0	32.0	36.0
16	34.21852037588332	36.0	36.0	36.0	32.0	36.0
17	34.13991788017964	36.0	36.0	36.0	32.0	36.0
18	34.14187722422233	36.0	36.0	36.0	32.0	36.0
19	34.155442444916105	36.0	36.0	36.0	32.0	36.0
20	34.123410190833205	36.0	36.0	36.0	32.0	36.0
21	34.099450088947314	36.0	36.0	36.0	32.0	36.0
22	34.096283202034265	36.0	36.0	36.0	32.0	36.0
23	34.034046839543535	36.0	36.0	36.0	32.0	36.0
24	34.02813687097072	36.0	36.0	36.0	32.0	36.0
25	33.62760810702334	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1.0
3	6.0
4	16.0
5	12.0
6	44.0
7	24.0
8	62.0
9	95.0
10	109.0
11	35.0
12	61.0
13	59.0
14	121.0
15	203.0
16	339.0
17	390.0
18	553.0
19	699.0
20	1132.0
21	1697.0
22	2542.0
23	3958.0
24	6009.0
25	8662.0
26	12539.0
27	16755.0
28	23152.0
29	31322.0
30	41359.0
31	56632.0
32	79535.0
33	112828.0
34	241686.0
35	515914.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.657110784906315	17.084621362576634	11.225714532423797	26.032553320093253
2	16.674435426927907	19.822682363015176	37.76230355826484	25.740578651792077
3	18.907588049915454	24.112336526202853	28.45883408040783	28.521241343473868
4	12.3455223603605	15.487945198148298	36.193753824985905	35.972778616505295
5	14.447471509049253	36.69415195288829	33.656354504945455	15.202022033117007
6	33.76119544714886	35.76975038738945	17.233154493933416	13.235899671528278
7	30.0153505591126	30.459808817333528	20.96033374083974	18.56450688271413
8	28.103426689059376	32.716871813248254	19.599694330873877	19.580007166818493
9	27.769986459225688	13.773679083648824	18.401117804736508	40.05521665238898
10	15.703398672037475	26.78604741144038	31.701305161918842	25.809248754603303
11	37.29528195233847	20.903415141384247	22.653175261881053	19.148127644396233
12	24.820824905035906	23.58745765698226	29.203165297122315	22.38855214085952
13	29.672480464481936	19.486803430408468	25.35648487643844	25.48423122867115
14	23.630532481206775	19.747765240802913	25.430086954568893	31.191615323421416
15	25.04093815747199	27.333302241061837	22.51261828018282	25.11314132128335
16	25.473332711436342	25.529821688312136	24.271603146798785	24.725242453452736
17	23.837076230379697	25.70131275625263	25.491948906237987	24.969662107129686
18	24.64091872284808	24.90784869130888	26.476343007601027	23.974889578242013
19	25.49177977111897	24.66595602056612	25.647528748341443	24.19473545997346
20	25.76458483969699	24.306036080163167	25.06200387184052	24.86737520829932
21	27.11474073024681	23.84367797822459	24.495869167855872	24.545712123672725
22	25.856611701613012	24.274177943026164	25.100122323736336	24.769088031624488
23	24.32109292053364	24.128618930007022	25.734584587049124	25.815703562410206
24	24.80157701783302	24.82576440790997	25.502579412384634	24.87007916187238
25	24.812392504165935	24.441280819071824	25.5750028291219	25.17132384764034
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	136.0
1	136.0
2	336.0
3	536.0
4	536.0
5	536.0
6	1524.0
7	2512.0
8	2512.0
9	2512.0
10	2485.0
11	2458.0
12	2458.0
13	2458.0
14	2610.5
15	2763.0
16	2763.0
17	2763.0
18	4982.5
19	7202.0
20	7202.0
21	7202.0
22	11755.0
23	16308.0
24	16308.0
25	16308.0
26	25146.0
27	33984.0
28	33984.0
29	33984.0
30	42934.5
31	51885.0
32	51885.0
33	51885.0
34	63186.0
35	74487.0
36	74487.0
37	74487.0
38	84398.5
39	94310.0
40	94310.0
41	94310.0
42	108197.5
43	122085.0
44	122085.0
45	122085.0
46	136621.0
47	151157.0
48	151157.0
49	151157.0
50	154165.0
51	157173.0
52	157173.0
53	157173.0
54	145535.0
55	133897.0
56	133897.0
57	133897.0
58	122466.0
59	111035.0
60	111035.0
61	111035.0
62	97257.5
63	83480.0
64	83480.0
65	83480.0
66	69086.0
67	54692.0
68	54692.0
69	54692.0
70	41229.5
71	27767.0
72	27767.0
73	27767.0
74	21265.0
75	14763.0
76	14763.0
77	14763.0
78	11890.5
79	9018.0
80	9018.0
81	9018.0
82	6175.0
83	3332.0
84	3332.0
85	3332.0
86	2592.0
87	1852.0
88	1852.0
89	1852.0
90	1358.0
91	864.0
92	864.0
93	864.0
94	578.0
95	292.0
96	292.0
97	292.0
98	427.5
99	563.0
100	563.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.038927936707145394
2	0.005610456509899003
3	0.002762070897181048
4	0.004315735776845388
5	0.009753562855670575
6	0.013465095623757606
7	0.024772323359092522
8	0.03763321597409178
9	0.048940443709426684
10	0.05929820957385562
11	0.05852137713402345
12	0.06430446307499627
13	0.06801599584308331
14	0.07077806674026435
15	0.06102450388459377
16	0.06931071657613691
17	0.06542655437697607
18	0.0814810914668409
19	0.07932322357841821
20	0.08303475634650524
21	0.07897796471627058
22	0.08346632992418979
23	0.08562419781261248
24	0.07966848244056585
25	0.08182635032898854
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1158551.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.4235090742924
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.48698405046753	42.171135796101694
2	13.749140731573494	14.965529707370266
3	4.152192282168275	6.779306230403758
4	1.6519543362817142	3.596206072437781
5	0.832755464120845	2.2660737279123695
6	0.4779636670246919	1.5607475981700234
7	0.3063268841360199	1.1669968770933477
8	0.22541330678099947	0.9814226517649585
9	0.15678561241552685	0.7679540880013425
>10	0.78830134895976	7.667531013622699
>50	0.0745631110598005	2.8384342170947456
>100	0.0859940821155031	9.769186914967042
>500	0.008440157718901794	3.1216256945878578
>1k	0.003184965176944073	2.347849410472127
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3248	0.28035019606387634	No Hit
TATCAACGCAGAGTACTTTTTTTTT	2484	0.21440575339367884	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1903	0.16425690366673543	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1309	0.11298596263781223	No Hit
GAGTACTTTTTTTTTTTTTTTTTTT	1287	0.11108703889600026	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1236	0.10668498840361797	No Hit
ACGCAGAGTACTTTTTTTTTTTTTT	1203	0.10383660279090001	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1193	0.10297345563553092	No Hit
GTATCTGATCGTCTTCGAACCTCCG	1183	0.10211030848016187	No Hit
CTATTGGAGCTGGAATTACCGCGGC	1169	0.10090190246264516	No Hit
GTCCTATTCCATTATTCCTAGCTGC	1164	0.10047032888496062	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	8.631471553690775E-5	0.0
12	0.0	0.0	0.0	8.631471553690775E-5	3.45258862147631E-4
13	0.0	0.0	0.0	8.631471553690775E-5	3.45258862147631E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGAACCG	160	0.0	13.060689	5
GTATAGA	95	7.3894626E-8	12.998758	1
GGTTCTA	220	0.0	12.955547	13
CGGTCCA	215	0.0	12.814943	10
GTTCTAG	60	4.09392E-4	12.665458	1
CGAGCCG	230	0.0	12.392797	15
GACGACC	85	3.9355564E-6	12.295599	15
GCGACGA	85	3.937457E-6	12.295067	13
CGTTATT	125	1.4169927E-9	12.155688	2
GCGTAAC	55	0.0030658576	12.091844	11
TACGCCT	55	0.0030708206	12.089232	5
CGAACGT	55	0.0030728076	12.088188	4
TCGAACG	55	0.0030757904	12.086622	3
AAGCTCG	120	1.0000804E-8	11.874893	7
CTTATAG	80	2.875295E-5	11.8707905	3
CGCGGTT	265	0.0	11.831103	10
TAGAACC	170	1.8189894E-12	11.732654	4
ATTCGAA	65	8.027136E-4	11.691192	1
GAACCGC	130	2.6157068E-9	11.691192	6
GTCTTAC	65	8.027136E-4	11.691192	1
>>END_MODULE