FastQCFastQC Report
Thu 2 Feb 2017
SRR4063932_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063932_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1158551
Sequences flagged as poor quality0
Sequence length50
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA13530.11678381012143618No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT12860.11100072418046335No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA12260.10582184124824888No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGTA407.032196E-427.499849
ATACCGT3900.019.1785476
GTATCAA25300.019.13281
TACCGTC3950.018.935787
TAATACT1901.8189894E-1218.5254084
TCTACCG600.00741401818.3324343
TACACCG600.00741401818.3324345
GTCCTAA2650.018.2664091
CGTCGTA3950.017.8226810
ACCGTCG4000.017.5991388
TCTATAC1254.854537E-717.5991383
TTAGGAC3550.017.3512613
TAAGACT1651.6607373E-917.3324834
ATTCGTG1154.272815E-617.2172944
TAGGACG3450.017.2165474
AAATTCG902.215371E-417.1110121
GTAGGAC4250.017.0815163
CCGTCGT4150.016.9637579
GTCGTAG4150.016.96375711
AACGCAG29950.016.2329256
Produced by FastQC (version 0.11.5)