##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063932_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1158551
Sequences flagged as poor quality	0
Sequence length	50
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.314800988476122	32.0	32.0	32.0	32.0	32.0
2	31.400436407201756	32.0	32.0	32.0	32.0	32.0
3	31.47838463736167	32.0	32.0	32.0	32.0	32.0
4	31.564790846497047	32.0	32.0	32.0	32.0	32.0
5	31.505245776836755	32.0	32.0	32.0	32.0	32.0
6	35.10005774454469	36.0	36.0	36.0	36.0	36.0
7	35.117591715858865	36.0	36.0	36.0	36.0	36.0
8	35.072824588645645	36.0	36.0	36.0	36.0	36.0
9	35.17806898444695	36.0	36.0	36.0	36.0	36.0
10	35.02662032141874	36.0	36.0	36.0	36.0	36.0
11	35.18631808181081	36.0	36.0	36.0	36.0	36.0
12	35.08012077154998	36.0	36.0	36.0	36.0	36.0
13	35.12455645025553	36.0	36.0	36.0	36.0	36.0
14	35.06719773233979	36.0	36.0	36.0	36.0	36.0
15	35.03763321597409	36.0	36.0	36.0	36.0	36.0
16	35.059722877974295	36.0	36.0	36.0	36.0	36.0
17	35.00816278264833	36.0	36.0	36.0	36.0	36.0
18	35.010412144135216	36.0	36.0	36.0	36.0	36.0
19	35.008448484356755	36.0	36.0	36.0	36.0	36.0
20	34.99635492956288	36.0	36.0	36.0	36.0	36.0
21	34.99547106687578	36.0	36.0	36.0	36.0	36.0
22	34.979299141772785	36.0	36.0	36.0	36.0	36.0
23	34.92605590949384	36.0	36.0	36.0	32.0	36.0
24	34.89768253620255	36.0	36.0	36.0	32.0	36.0
25	34.87467534877619	36.0	36.0	36.0	32.0	36.0
26	34.811209001589056	36.0	36.0	36.0	32.0	36.0
27	34.81098458332866	36.0	36.0	36.0	32.0	36.0
28	34.77877797352037	36.0	36.0	36.0	32.0	36.0
29	34.74921777289045	36.0	36.0	36.0	32.0	36.0
30	34.728249339045064	36.0	36.0	36.0	32.0	36.0
31	34.73058933098327	36.0	36.0	36.0	32.0	36.0
32	34.68400959474378	36.0	36.0	36.0	32.0	36.0
33	34.65450118294318	36.0	36.0	36.0	32.0	36.0
34	34.641560017642725	36.0	36.0	36.0	32.0	36.0
35	34.59374857041252	36.0	36.0	36.0	32.0	36.0
36	34.579472979609875	36.0	36.0	36.0	32.0	36.0
37	34.56577914998994	36.0	36.0	36.0	32.0	36.0
38	34.52244829964326	36.0	36.0	36.0	32.0	36.0
39	34.51228128930017	36.0	36.0	36.0	32.0	36.0
40	34.47800140002469	36.0	36.0	36.0	32.0	36.0
41	34.464506957397646	36.0	36.0	36.0	32.0	36.0
42	34.39761823173947	36.0	36.0	36.0	32.0	36.0
43	34.38365164761845	36.0	36.0	36.0	32.0	36.0
44	34.30422743582285	36.0	36.0	36.0	32.0	36.0
45	34.27911416933739	36.0	36.0	36.0	32.0	36.0
46	34.24298887144373	36.0	36.0	36.0	32.0	36.0
47	34.219875516917256	36.0	36.0	36.0	32.0	36.0
48	34.16209472004254	36.0	36.0	36.0	32.0	36.0
49	34.13726542897119	36.0	36.0	36.0	32.0	36.0
50	33.52183201257433	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	2.0
17	1.0
18	2.0
19	6.0
20	12.0
21	28.0
22	99.0
23	351.0
24	843.0
25	2034.0
26	4291.0
27	7803.0
28	13233.0
29	20432.0
30	29108.0
31	42452.0
32	61375.0
33	98554.0
34	205061.0
35	672864.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.87981830831994	17.945074694892984	11.759719486277307	26.41538751050977
2	15.983655374926848	20.703623334766164	37.16106222799958	26.15165906230741
3	18.17851467654799	24.690453317037086	28.846082470400475	28.284949536014448
4	12.050052177245542	16.164674666889933	36.22835766401307	35.55691549185146
5	14.09415727059059	37.27992984339921	33.35079767744363	15.275115208566564
6	33.801533121977364	36.213511532940714	16.869952207542006	13.11500313753991
7	29.939579646972508	30.642095723102152	20.945923784040396	18.47240084588494
8	28.070149695611157	33.10644071775865	19.39379448984119	19.429615096789007
9	27.885130790178973	13.623239694959583	18.247614444103977	40.24401507075746
10	15.663925575357283	27.01181917601535	31.644024682769107	25.680230565858263
11	37.56563155182638	20.8790981148003	22.59261784763899	18.962652485734335
12	24.763648322168503	23.77827782855969	29.09889870864331	22.359175140628494
13	29.822061292396928	19.420738944106617	25.337298076466602	25.41990168702985
14	23.72981422483775	19.733356580763385	25.436946668726712	31.099882525672157
15	25.14615895223043	27.512324136663047	22.31758508207603	25.023931829030495
16	25.614564487839907	25.556646963775222	24.103443632697594	24.725344915687273
17	23.976329052411156	25.781601327865584	25.2837380486487	24.958331571074556
18	24.804410348125412	24.868801292652527	26.487810604308148	23.83897775491391
19	25.640908341540424	24.603319146071257	25.666284867908274	24.089487644480045
20	26.004990751314367	24.19970601004883	25.09384632249299	24.701456916143812
21	27.273205927058886	23.91573612210425	24.433020212316936	24.37803773851993
22	25.969057820651365	24.276162921173743	25.145613302167884	24.609165956007
23	24.588318766173344	24.052731795653504	25.657852714877837	25.701096723295315
24	24.910577723150013	24.83505215148617	25.466704875413015	24.7876652499508
25	24.941434606148803	24.374338603787198	25.649570923253005	25.03465586681099
26	24.62101005287991	25.249854550037377	25.92859226879498	24.20054312828773
27	25.312112368221378	24.633117446440455	25.149053455528247	24.905716729809914
28	24.516290532112784	24.88789334438215	25.71025338909533	24.88556273440973
29	24.54556204947869	24.5912237952075	25.74597395467834	25.117240200635464
30	24.40341267114715	25.14747441964047	25.854419398777388	24.594693510434993
31	25.181793697818478	24.787216778194423	24.841513186517226	25.189476337469873
32	24.76148139196637	25.054704122986088	24.73834812970164	25.445466355345896
33	24.502826502224	24.54417310675279	25.429974475592985	25.523025915430225
34	25.250257443416395	24.671667931225276	25.522590713737596	24.555483911620733
35	25.649929651017256	24.575877635928908	25.58691767874252	24.187275034311316
36	24.487687229384914	25.205701993660618	25.17126007361421	25.135350703340265
37	25.426240828657747	25.161933534743202	24.859732412602504	24.552093223996547
38	24.677565684581072	24.966744442804327	25.32411759475784	25.03157227785676
39	25.426593467301984	24.766159105034184	24.721876942200126	25.08537048546371
40	25.772823909311178	25.004251146514793	25.14434476668758	24.078580177486447
41	24.52072058074596	25.391666882461095	25.71535852085869	24.37225401593426
42	25.760302593224626	25.5318212303812	24.977061230760995	23.730814945633174
43	25.014177436771224	24.576209009512908	25.37109494696301	25.038518606752863
44	24.64452767259171	25.289742423366185	24.803177132380803	25.262552771661305
45	24.817936600418637	25.452277680671543	24.92660926609266	24.803176452817162
46	24.604543328033532	25.20504361546575	24.86711654556534	25.32329651093537
47	25.018148232867077	24.962905098337096	25.27321614307972	24.745730525716112
48	25.773979845061607	25.40937830430936	24.258432058004793	24.55820979262424
49	24.8076033366018	25.714172271402724	24.47287284729799	25.005351544697486
50	24.869770424787944	25.871642623287595	24.36119534134175	24.897391610582712
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	21.0
1	20.5
2	20.0
3	71.0
4	122.0
5	148.5
6	175.0
7	180.5
8	186.0
9	254.5
10	323.0
11	492.5
12	662.0
13	1255.5
14	1849.0
15	2488.0
16	3127.0
17	3456.5
18	3786.0
19	3884.0
20	3982.0
21	4293.0
22	4604.0
23	5286.0
24	5968.0
25	6470.0
26	6972.0
27	8753.0
28	10534.0
29	12549.5
30	14565.0
31	16723.0
32	18881.0
33	21869.5
34	24858.0
35	28969.5
36	33081.0
37	38373.5
38	43666.0
39	47023.0
40	50380.0
41	54805.5
42	59231.0
43	60745.0
44	62259.0
45	68459.5
46	74660.0
47	79810.0
48	84960.0
49	88579.0
50	92198.0
51	88883.0
52	85568.0
53	84010.0
54	82452.0
55	82768.0
56	83084.0
57	80898.0
58	78712.0
59	71592.5
60	64473.0
61	56308.0
62	48143.0
63	41733.5
64	35324.0
65	30133.0
66	24942.0
67	21399.5
68	17857.0
69	16074.5
70	14292.0
71	11058.5
72	7825.0
73	6734.5
74	5644.0
75	4356.0
76	3068.0
77	2722.0
78	2376.0
79	1966.5
80	1557.0
81	1251.5
82	946.0
83	781.5
84	617.0
85	459.5
86	302.0
87	214.5
88	127.0
89	95.0
90	63.0
91	48.5
92	34.0
93	32.0
94	30.0
95	26.5
96	23.0
97	17.5
98	12.0
99	11.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.009063045131375312
2	4.3157357768453875E-4
3	1.726294310738155E-4
4	0.0
5	0.0
6	0.0
7	8.631471553690775E-5
8	0.0
9	0.001381035448590524
10	8.631471553690775E-4
11	0.0
12	1.726294310738155E-4
13	5.178882932214465E-4
14	0.0
15	0.004488365207919202
16	6.042030087583542E-4
17	0.0
18	2.5894414661072323E-4
19	0.0
20	6.90517724295262E-4
21	0.0
22	2.5894414661072323E-4
23	7.768324398321696E-4
24	2.5894414661072323E-4
25	0.0014673501641274316
26	0.006646233096341896
27	0.0063872889497311736
28	0.004833624070066833
29	0.002762070897181048
30	0.0038841621991608484
31	0.008199897976006235
32	0.003970476914697756
33	0.004488365207919202
34	0.003797847483623941
35	0.0035389033370132176
36	0.006818862527415711
37	0.004402050492382295
38	0.008458842122616958
39	0.0061283448031204495
40	0.00345258862147631
41	0.0035389033370132176
42	0.002762070897181048
43	0.001726294310738155
44	0.0018989237418119704
45	0.002244182603959601
46	0.0014673501641274316
47	0.0031073297593286783
48	0.002244182603959601
49	6.042030087583542E-4
50	0.001726294310738155
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1158551.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.810901783163395
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.32022022949792	45.27762559323231
2	13.392067954088946	15.484150503345717
3	4.036713554446799	7.000981524686649
4	1.5882579412368507	3.6727449538869155
5	0.7842175798998618	2.2668162744110503
6	0.43844353391287716	1.5208089645900247
7	0.2861477325698534	1.157972092414949
8	0.19783372562987256	0.9149556865428683
9	0.1400666318750004	0.7287640468581734
>10	0.6716817951809302	6.944817935665816
>50	0.06407843766822166	2.597215746042495
>100	0.07155232771108719	8.539794165316778
>500	0.00721535692286857	2.8942481461915572
>1k	0.001503199358930952	0.9991043668147227
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1353	0.11678381012143618	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1286	0.11100072418046335	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	1226	0.10582184124824888	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.631471553690775E-5
2	0.0	0.0	0.0	0.0	8.631471553690775E-5
3	0.0	0.0	0.0	8.631471553690775E-5	8.631471553690775E-5
4	0.0	0.0	0.0	8.631471553690775E-5	8.631471553690775E-5
5	0.0	0.0	0.0	8.631471553690775E-5	8.631471553690775E-5
6	0.0	0.0	0.0	8.631471553690775E-5	8.631471553690775E-5
7	0.0	0.0	0.0	8.631471553690775E-5	8.631471553690775E-5
8	0.0	0.0	0.0	8.631471553690775E-5	8.631471553690775E-5
9	0.0	0.0	0.0	8.631471553690775E-5	8.631471553690775E-5
10	0.0	0.0	0.0	8.631471553690775E-5	8.631471553690775E-5
11	0.0	0.0	0.0	8.631471553690775E-5	8.631471553690775E-5
12	0.0	0.0	0.0	8.631471553690775E-5	3.45258862147631E-4
13	0.0	0.0	0.0	8.631471553690775E-5	3.45258862147631E-4
14	0.0	0.0	0.0	8.631471553690775E-5	3.45258862147631E-4
15	0.0	0.0	0.0	1.726294310738155E-4	3.45258862147631E-4
16	0.0	0.0	0.0	3.45258862147631E-4	3.45258862147631E-4
17	0.0	0.0	0.0	3.45258862147631E-4	4.315735776845387E-4
18	0.0	0.0	0.0	3.45258862147631E-4	5.178882932214465E-4
19	0.0	0.0	0.0	3.45258862147631E-4	5.178882932214465E-4
20	0.0	0.0	0.0	3.45258862147631E-4	6.042030087583542E-4
21	0.0	0.0	0.0	4.315735776845387E-4	6.042030087583542E-4
22	0.0	0.0	0.0	5.178882932214465E-4	6.042030087583542E-4
23	0.0	0.0	0.0	0.001035776586442893	6.042030087583542E-4
24	0.0	0.0	0.0	0.0017262943107381548	6.042030087583542E-4
25	0.0	0.0	0.0	0.0018989237418119702	6.042030087583542E-4
26	0.0	0.0	0.0	0.0021578678884226934	6.042030087583542E-4
27	0.0	8.631471553690775E-5	0.0	0.00267575618164414	6.042030087583542E-4
28	0.0	8.631471553690775E-5	0.0	0.003625218052550125	6.042030087583542E-4
29	0.0	8.631471553690775E-5	0.0	0.005178882932214465	6.042030087583542E-4
30	0.0	8.631471553690775E-5	0.0	0.010098821717818205	6.042030087583542E-4
31	0.0	8.631471553690775E-5	0.0	0.023909176203723445	6.042030087583542E-4
32	0.0	8.631471553690775E-5	0.0	0.03866899256053467	6.042030087583542E-4
33	0.0	8.631471553690775E-5	0.0	0.052047773468755366	6.042030087583542E-4
34	0.0	8.631471553690775E-5	0.0	0.06646233096341896	6.042030087583542E-4
35	0.0	8.631471553690775E-5	0.0	0.08294844163096834	6.042030087583542E-4
36	0.0	8.631471553690775E-5	0.0	0.11065546531831573	6.042030087583542E-4
37	0.0	8.631471553690775E-5	0.0	0.1482886812924075	6.042030087583542E-4
38	0.0	8.631471553690775E-5	0.0	0.20128591663206885	6.042030087583542E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTGTA	40	7.032196E-4	27.49984	9
ATACCGT	390	0.0	19.178547	6
GTATCAA	2530	0.0	19.1328	1
TACCGTC	395	0.0	18.93578	7
TAATACT	190	1.8189894E-12	18.525408	4
TCTACCG	60	0.007414018	18.332434	3
TACACCG	60	0.007414018	18.332434	5
GTCCTAA	265	0.0	18.266409	1
CGTCGTA	395	0.0	17.82268	10
ACCGTCG	400	0.0	17.599138	8
TCTATAC	125	4.854537E-7	17.599138	3
TTAGGAC	355	0.0	17.351261	3
TAAGACT	165	1.6607373E-9	17.332483	4
ATTCGTG	115	4.272815E-6	17.21729	44
TAGGACG	345	0.0	17.216547	4
AAATTCG	90	2.215371E-4	17.11101	21
GTAGGAC	425	0.0	17.081516	3
CCGTCGT	415	0.0	16.963757	9
GTCGTAG	415	0.0	16.963757	11
AACGCAG	2995	0.0	16.232925	6
>>END_MODULE