FastQCFastQC Report
Thu 2 Feb 2017
SRR4063931_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063931_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1395055
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT21730.15576446806756722No Hit
TATCAACGCAGAGTACTTTTTTTTT14220.1019314650676855No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTGCG500.001500841813.2983197
GGTATCA5200.013.1531271
CGACCAT1501.8189894E-1212.66597410
TTTAGAC701.0938632E-412.2105513
GTATCAA12950.012.176941
CTCGAAC550.003064486412.0928518
AGTGTCG550.003069430612.09024810
CCTATTC1650.012.0872123
GGTCTAC1900.011.9993441
TAGCACA1052.7246278E-711.7599964
ATAGCAC658.04312E-411.6887323
CCGCGCG752.073886E-411.3997859
GTCCTAT2000.011.3993761
CCTACAC2000.011.3965153
GTCTTAA1104.9696973E-711.2266591
TAGACAG2200.011.2250485
GTCCTAC3050.011.2125021
GTCTAAC855.3246848E-511.17585851
TGGACTA855.3324715E-511.1742555
AAGGTAC600.00588224911.0815346
Produced by FastQC (version 0.11.5)