##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063931_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1395055
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.245210403890887	32.0	32.0	32.0	32.0	32.0
2	30.835878872159164	32.0	32.0	32.0	32.0	32.0
3	30.84909125446667	32.0	32.0	32.0	32.0	32.0
4	30.881506463902856	32.0	32.0	32.0	32.0	32.0
5	30.778992942930564	32.0	32.0	32.0	32.0	32.0
6	34.42622477250001	36.0	36.0	36.0	32.0	36.0
7	34.38844418320425	36.0	36.0	36.0	32.0	36.0
8	34.33641325969227	36.0	36.0	36.0	32.0	36.0
9	34.48987817684608	36.0	36.0	36.0	32.0	36.0
10	34.18391963040884	36.0	36.0	36.0	32.0	36.0
11	34.457731774016075	36.0	36.0	36.0	32.0	36.0
12	34.28259746031519	36.0	36.0	36.0	32.0	36.0
13	34.35357530706675	36.0	36.0	36.0	32.0	36.0
14	34.25146607123017	36.0	36.0	36.0	32.0	36.0
15	34.18044306496876	36.0	36.0	36.0	32.0	36.0
16	34.185637125418	36.0	36.0	36.0	32.0	36.0
17	34.12118446942952	36.0	36.0	36.0	32.0	36.0
18	34.11161638788435	36.0	36.0	36.0	32.0	36.0
19	34.12842217690342	36.0	36.0	36.0	32.0	36.0
20	34.108021547537554	36.0	36.0	36.0	32.0	36.0
21	34.10343678206235	36.0	36.0	36.0	32.0	36.0
22	34.0793287719839	36.0	36.0	36.0	32.0	36.0
23	34.02364781316866	36.0	36.0	36.0	32.0	36.0
24	34.00076986212013	36.0	36.0	36.0	32.0	36.0
25	33.60172322955009	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	10.0
5	15.0
6	32.0
7	24.0
8	72.0
9	102.0
10	136.0
11	35.0
12	81.0
13	73.0
14	120.0
15	254.0
16	412.0
17	545.0
18	758.0
19	893.0
20	1413.0
21	2119.0
22	3155.0
23	4971.0
24	7430.0
25	10999.0
26	15638.0
27	20672.0
28	28389.0
29	38371.0
30	50128.0
31	68351.0
32	96529.0
33	135342.0
34	287938.0
35	620045.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.593871929815755	16.78528735368422	11.159188842460493	26.461651874039532
2	16.873394700603374	19.254273689613324	37.436352544715405	26.435979065067894
3	19.37041006139631	22.74609597608662	28.3464572573448	29.53703670517227
4	12.962319131472647	15.251128132157232	35.21851176160659	36.568040974763534
5	14.864185777769496	36.04835131118737	33.96506128862754	15.122401622415602
6	35.12445721798814	34.82809124974819	16.463509492098698	13.583942040164972
7	30.66288201967653	29.944979702572063	20.47509317028474	18.91704510746667
8	28.088161057347566	33.59087727953282	18.93890938086196	19.382052282257646
9	27.257275756943354	14.166829820330165	18.10173760444524	40.474156818281244
10	15.770048133163398	27.18057000968975	32.01153015548783	25.03785170165902
11	37.26209371160705	20.855282833882832	22.292707661699534	19.58991579281058
12	24.569063214581846	23.226043845379195	29.23376742611385	22.971125513925113
13	30.111572959964995	19.534771958641613	24.63477877280503	25.718876308588356
14	23.594483664893975	19.030079319194844	25.207909826599543	32.167527189311635
15	25.27200049920851	27.181759953866248	21.891153896085733	25.65508565083951
16	25.83683493984027	25.553215572238297	23.59140735172388	25.018542136197546
17	24.379241734579846	25.640483093312067	25.044004297860685	24.936270874247406
18	24.80392037298352	25.235571746168617	25.67396655114355	24.286541329704313
19	25.719766506762394	24.87182191154833	24.90883878220235	24.49957279948693
20	25.898853754316892	24.62692134966878	24.79414495007683	24.680079945937493
21	26.33332711618558	24.27119485449722	24.436403600015495	24.959074429301705
22	25.936543396399674	24.418890775642087	24.756210682179653	24.888355145778586
23	25.030634089787295	24.457482466108797	24.834776794425885	25.677106649678016
24	25.036137819420663	24.762602324728885	25.037357358736102	25.163902497114353
25	25.28398289143869	24.340816774539753	25.01165775416415	25.363542579857413
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	53.0
1	53.0
2	148.0
3	243.0
4	243.0
5	243.0
6	638.0
7	1033.0
8	1033.0
9	1033.0
10	1375.5
11	1718.0
12	1718.0
13	1718.0
14	2453.0
15	3188.0
16	3188.0
17	3188.0
18	5631.0
19	8074.0
20	8074.0
21	8074.0
22	12608.5
23	17143.0
24	17143.0
25	17143.0
26	25631.0
27	34119.0
28	34119.0
29	34119.0
30	45153.0
31	56187.0
32	56187.0
33	56187.0
34	68761.5
35	81336.0
36	81336.0
37	81336.0
38	97090.5
39	112845.0
40	112845.0
41	112845.0
42	129931.0
43	147017.0
44	147017.0
45	147017.0
46	162968.0
47	178919.0
48	178919.0
49	178919.0
50	186624.0
51	194329.0
52	194329.0
53	194329.0
54	185269.0
55	176209.0
56	176209.0
57	176209.0
58	161715.0
59	147221.0
60	147221.0
61	147221.0
62	126374.0
63	105527.0
64	105527.0
65	105527.0
66	86476.5
67	67426.0
68	67426.0
69	67426.0
70	50526.0
71	33626.0
72	33626.0
73	33626.0
74	24684.0
75	15742.0
76	15742.0
77	15742.0
78	11717.5
79	7693.0
80	7693.0
81	7693.0
82	5197.0
83	2701.0
84	2701.0
85	2701.0
86	2007.5
87	1314.0
88	1314.0
89	1314.0
90	907.5
91	501.0
92	501.0
93	501.0
94	365.0
95	229.0
96	229.0
97	229.0
98	445.5
99	662.0
100	662.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03971169595463978
2	0.004730996268964306
3	0.0014336352330194867
4	0.003584088082548717
5	0.007024812641795485
6	0.011325718340853944
7	0.021289483210339376
8	0.03376210973760891
9	0.04759668973624696
10	0.05720204579747752
11	0.054191411808136594
12	0.06329499553781034
13	0.06451358548587689
14	0.06745253771356684
15	0.06078613388002624
16	0.06687908362035906
17	0.06257817792130059
18	0.07928002838597761
19	0.07856321076946787
20	0.07978180071753443
21	0.07605434911168377
22	0.0807136636189971
23	0.08479952403310263
24	0.07784639315295813
25	0.08121543595055392
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1395055.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.39458579626657
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.50264689296914	36.41562395701291
2	16.103154238745983	16.8743619270498
3	6.275969205985059	9.864804210531334
4	2.9090925103120573	6.096827884832863
5	1.60164551194864	4.195877659549915
6	0.9508548771713511	2.989178846505172
7	0.618453804964767	2.2682541661677798
8	0.4163860845607688	1.7453101145512566
9	0.29024870962330523	1.3686714826752535
>10	1.2275994015953817	10.786516020600427
>50	0.06013483499210841	2.17143556763939
>100	0.041058364982237715	4.193372509536799
>500	0.0024800059343604896	0.7702488272844926
>1k	2.755562149289433E-4	0.2595168260625821
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2173	0.15576446806756722	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1422	0.1019314650676855	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	7.168176165097434E-5	0.0
6	0.0	0.0	0.0	7.168176165097434E-5	0.0
7	0.0	0.0	0.0	7.168176165097434E-5	0.0
8	0.0	0.0	0.0	7.168176165097434E-5	0.0
9	0.0	0.0	0.0	1.4336352330194868E-4	0.0
10	0.0	0.0	0.0	1.4336352330194868E-4	0.0
11	0.0	0.0	0.0	1.4336352330194868E-4	0.0
12	0.0	0.0	0.0	1.4336352330194868E-4	7.168176165097434E-5
13	0.0	0.0	0.0	1.4336352330194868E-4	7.168176165097434E-5
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGTGCG	50	0.0015008418	13.298319	7
GGTATCA	520	0.0	13.153127	1
CGACCAT	150	1.8189894E-12	12.665974	10
TTTAGAC	70	1.0938632E-4	12.210551	3
GTATCAA	1295	0.0	12.17694	1
CTCGAAC	55	0.0030644864	12.09285	18
AGTGTCG	55	0.0030694306	12.090248	10
CCTATTC	165	0.0	12.087212	3
GGTCTAC	190	0.0	11.999344	1
TAGCACA	105	2.7246278E-7	11.759996	4
ATAGCAC	65	8.04312E-4	11.688732	3
CCGCGCG	75	2.073886E-4	11.399785	9
GTCCTAT	200	0.0	11.399376	1
CCTACAC	200	0.0	11.396515	3
GTCTTAA	110	4.9696973E-7	11.226659	1
TAGACAG	220	0.0	11.225048	5
GTCCTAC	305	0.0	11.212502	1
GTCTAAC	85	5.3246848E-5	11.1758585	1
TGGACTA	85	5.3324715E-5	11.174255	5
AAGGTAC	60	0.005882249	11.081534	6
>>END_MODULE