FastQCFastQC Report
Thu 2 Feb 2017
SRR4063931_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063931_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1395055
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13400.029.2242151
GGTATCA4400.026.5003011
CGCCCTA508.339651E-526.40124724
TATACGG450.001399104624.4420975
TAATACC1102.0190782E-1023.9976924
TCAACGC19700.019.0946124
TATCAAC20500.018.7786832
CAACGCA20200.018.7308715
ATCAACG20450.018.501893
AACGCAG20750.018.3404056
TAGGACG2950.017.8965854
CGCGGTT2400.017.4174910
TACGAAT1702.4683686E-916.82311841
ATACGAA1702.4683686E-916.82311840
TATACTG2500.016.7183935
TACGACG2007.2759576E-1116.4984155
CGCAATA1753.621608E-916.344836
ATACCGT2700.016.2947316
GTATAGA2301.8189894E-1216.2610551
GTGTATA4200.016.238281
Produced by FastQC (version 0.11.5)