##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4063931_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1395055
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.328442247796684	32.0	32.0	32.0	32.0	32.0
2	31.379740583704585	32.0	32.0	32.0	32.0	32.0
3	31.450294074427173	32.0	32.0	32.0	32.0	32.0
4	31.535752353849848	32.0	32.0	32.0	32.0	32.0
5	31.4788728759798	32.0	32.0	32.0	32.0	32.0
6	35.054641573271304	36.0	36.0	36.0	36.0	36.0
7	35.07847575902025	36.0	36.0	36.0	36.0	36.0
8	35.01547967642853	36.0	36.0	36.0	36.0	36.0
9	35.13487855317532	36.0	36.0	36.0	36.0	36.0
10	34.985860055696726	36.0	36.0	36.0	36.0	36.0
11	35.132378293328934	36.0	36.0	36.0	36.0	36.0
12	35.0385246459817	36.0	36.0	36.0	36.0	36.0
13	35.084284848984446	36.0	36.0	36.0	36.0	36.0
14	35.026051302636816	36.0	36.0	36.0	36.0	36.0
15	34.99298522280483	36.0	36.0	36.0	36.0	36.0
16	35.012985151123075	36.0	36.0	36.0	36.0	36.0
17	34.97736074921777	36.0	36.0	36.0	36.0	36.0
18	34.97179681087842	36.0	36.0	36.0	36.0	36.0
19	34.97004777589414	36.0	36.0	36.0	36.0	36.0
20	34.96526015103347	36.0	36.0	36.0	36.0	36.0
21	34.965751171100784	36.0	36.0	36.0	36.0	36.0
22	34.95058115988259	36.0	36.0	36.0	36.0	36.0
23	34.89588940937813	36.0	36.0	36.0	32.0	36.0
24	34.873468071151315	36.0	36.0	36.0	32.0	36.0
25	34.845143023034936	36.0	36.0	36.0	32.0	36.0
26	34.7827325804359	36.0	36.0	36.0	32.0	36.0
27	34.778605144600036	36.0	36.0	36.0	32.0	36.0
28	34.76479565321797	36.0	36.0	36.0	32.0	36.0
29	34.73980667428883	36.0	36.0	36.0	32.0	36.0
30	34.72284461902936	36.0	36.0	36.0	32.0	36.0
31	34.705375056897395	36.0	36.0	36.0	32.0	36.0
32	34.66842024149585	36.0	36.0	36.0	32.0	36.0
33	34.660700832583665	36.0	36.0	36.0	32.0	36.0
34	34.65515624831996	36.0	36.0	36.0	32.0	36.0
35	34.62848418162725	36.0	36.0	36.0	32.0	36.0
36	34.60493743974252	36.0	36.0	36.0	32.0	36.0
37	34.58581991391021	36.0	36.0	36.0	32.0	36.0
38	34.55522183713187	36.0	36.0	36.0	32.0	36.0
39	34.55446631136407	36.0	36.0	36.0	32.0	36.0
40	34.54085967936748	36.0	36.0	36.0	32.0	36.0
41	34.52665952238442	36.0	36.0	36.0	32.0	36.0
42	34.48096454978477	36.0	36.0	36.0	32.0	36.0
43	34.46299106486841	36.0	36.0	36.0	32.0	36.0
44	34.41816344158474	36.0	36.0	36.0	32.0	36.0
45	34.398975667626004	36.0	36.0	36.0	32.0	36.0
46	34.380135550211286	36.0	36.0	36.0	32.0	36.0
47	34.35049155768052	36.0	36.0	36.0	32.0	36.0
48	34.3094996254628	36.0	36.0	36.0	32.0	36.0
49	34.29597542749211	36.0	36.0	36.0	32.0	36.0
50	33.69494679421241	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	1.0
18	0.0
19	3.0
20	10.0
21	54.0
22	123.0
23	392.0
24	1086.0
25	2580.0
26	5188.0
27	9568.0
28	15825.0
29	24457.0
30	35106.0
31	50432.0
32	72986.0
33	116394.0
34	243598.0
35	817252.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.620824500897164	17.690058374726604	11.698386970743817	26.990730153632413
2	16.16300491021827	20.015985090140138	36.9027633418157	26.918246657825883
3	18.570073782248823	23.28323480646944	28.676155925482295	29.47053548579944
4	12.736343728383467	15.821168341033149	35.17997498306518	36.262512947518196
5	14.51756382364853	36.532824870704026	33.624982527570594	15.324628778076848
6	35.2334072374415	35.11341162437789	16.277039334045853	13.37614180413476
7	30.628183118228314	30.036521857561173	20.44808269208024	18.887212332130275
8	28.009935092164824	33.782395676156135	18.84198114052851	19.365688091150528
9	27.250434037111425	14.03886047875261	17.89437163899008	40.81633384514588
10	15.592339739821826	27.306880643191185	32.120492256875046	24.980287360111937
11	37.47837898864203	20.80111536821129	22.196400858747506	19.52410478439918
12	24.471884242473767	23.258812920503434	29.212775992900635	23.056526844122164
13	30.23255813953488	19.45687929241195	24.584799530339076	25.72576303771409
14	23.579618765805908	18.966444165362052	25.155263474805167	32.29867359402687
15	25.322244070357847	27.193664725663325	21.79402903117816	25.69006217280067
16	25.8347221774208	25.59731048102391	23.518596174316958	25.049371167238334
17	24.33939880506503	25.713896584722466	25.011773729351173	24.934930880861327
18	24.90550538223279	25.175029192439286	25.635083398265156	24.28438202706277
19	25.692392056227177	24.951489367802704	24.92711756884137	24.42900100712875
20	25.970541473184394	24.59725342390143	24.826134765448597	24.606070337465574
21	26.411145080301495	24.36721132858561	24.37151223428467	24.850131356828225
22	26.013401631764793	24.400560838505175	24.737321996137783	24.848715533592248
23	25.152128363436432	24.399532916284063	24.816582188741677	25.63175653153783
24	25.13562257356019	24.78767755661454	24.9196443419868	25.157055527838466
25	25.407392380527742	24.30118040387633	25.000878116687016	25.290549098908915
26	24.906844943246742	24.970429810765125	25.300038853436526	24.82268639255161
27	25.25027258474859	24.771197272503745	24.775785155188714	25.20274498755895
28	25.009677336108936	24.67799560147153	25.0426519628505	25.269675099569035
29	25.11223813193312	24.750094084335405	24.916686080894607	25.220981702836866
30	25.101593889671435	24.89980394476045	25.03499962365996	24.963602541908152
31	25.408935870654542	24.47693915676556	24.80927795882612	25.30484701375378
32	25.206090235265442	24.825880632535736	24.66810511677252	25.2999240154263
33	24.908350860290437	24.718099145946148	24.908709284771117	25.4648407089923
34	25.52638615199232	24.63572494087945	25.027185775799964	24.810703131328264
35	25.39855945130693	24.50552822030302	25.23970913762057	24.856203190769477
36	25.074966253536836	24.793954813073626	24.938116549494932	25.192962383894603
37	25.59536866190974	24.58405554623668	24.732942514003405	25.08763327785018
38	25.089107519728394	24.668588846577354	24.82916876949899	25.413134864195264
39	25.64687986557745	24.41448196564311	24.63311933155747	25.305518837221975
40	25.6558088590019	24.636119719031736	24.841992985112178	24.866078436854185
41	25.07883439079062	24.683497573140894	25.27202255462852	24.965645481439957
42	25.674304978606177	24.812387412734786	24.879411109016374	24.63389649964266
43	25.20990452596666	24.27466185533164	25.12904657145786	25.38638704724384
44	24.986398229726912	24.624114451280573	24.93808416825623	25.45140315073629
45	25.079961750255546	24.686783623718856	24.88541563623174	25.347838989793857
46	25.021504698059704	24.56517500523281	24.8191454893179	25.594174807389585
47	25.164244132909257	24.566692974401214	25.204601774732676	25.064461117956853
48	25.175391122022905	24.864500636189316	24.60622569488002	25.353882546907762
49	25.126679956503384	24.694366012425352	24.793287127137287	25.38566690393398
50	25.121716940016057	24.975627938983827	24.783375387085673	25.11927973391444
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	8.0
1	13.0
2	18.0
3	56.0
4	94.0
5	122.0
6	150.0
7	146.0
8	142.0
9	183.5
10	225.0
11	343.5
12	462.0
13	872.0
14	1282.0
15	1671.0
16	2060.0
17	2095.0
18	2130.0
19	2199.5
20	2269.0
21	2673.5
22	3078.0
23	3813.0
24	4548.0
25	5944.0
26	7340.0
27	9326.0
28	11312.0
29	13583.5
30	15855.0
31	18446.0
32	21037.0
33	25043.0
34	29049.0
35	33276.0
36	37503.0
37	42469.5
38	47436.0
39	51787.5
40	56139.0
41	61487.0
42	66835.0
43	71356.0
44	75877.0
45	83320.0
46	90763.0
47	96245.0
48	101727.0
49	106233.0
50	110739.0
51	110980.5
52	111222.0
53	111305.0
54	111388.0
55	108849.0
56	106310.0
57	101930.0
58	97550.0
59	90253.0
60	82956.0
61	73292.0
62	63628.0
63	54730.0
64	45832.0
65	38722.5
66	31613.0
67	26424.5
68	21236.0
69	17711.5
70	14187.0
71	10935.5
72	7684.0
73	6442.5
74	5201.0
75	4174.5
76	3148.0
77	2605.0
78	2062.0
79	1686.5
80	1311.0
81	1028.0
82	745.0
83	617.0
84	489.0
85	353.5
86	218.0
87	143.5
88	69.0
89	60.5
90	52.0
91	43.0
92	34.0
93	22.0
94	10.0
95	13.0
96	16.0
97	11.5
98	7.0
99	8.0
100	9.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0073115396883993815
2	3.5840880825487167E-4
3	2.8672704660389736E-4
4	0.0
5	0.0
6	2.8672704660389736E-4
7	0.0
8	0.0
9	9.318629014626663E-4
10	7.884993781607176E-4
11	0.0
12	7.168176165097434E-5
13	4.30090569905846E-4
14	5.017723315568203E-4
15	0.005161086838870152
16	7.168176165097433E-4
17	0.0
18	1.4336352330194868E-4
19	0.0
20	6.45135854858769E-4
21	0.0
22	7.168176165097434E-5
23	0.0011469081864155894
24	5.017723315568203E-4
25	0.001720362279623384
26	0.004946041553917229
27	0.005447813885474049
28	0.0030823157509918967
29	0.00215045284952923
30	0.0028672704660389734
31	0.006666403833540614
32	0.0025088616577841017
33	0.004372587460709434
34	0.001720362279623384
35	0.0022221346111802043
36	0.0067380855951915865
37	0.0026522251810860505
38	0.007956675543258152
39	0.0036557698441996907
40	0.0015769987563214352
41	0.003010633989340922
42	0.0018637258029253327
43	0.0011469081864155894
44	0.0011469081864155894
45	9.318629014626663E-4
46	7.884993781607176E-4
47	0.0020070893262272814
48	0.00215045284952923
49	2.8672704660389736E-4
50	0.001075226424764615
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1395055.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.9350169469741
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.69131125117188	38.83428381586948
2	15.591096179625435	17.12994265699253
3	6.089341702936612	10.035542689402169
4	2.8512600372660453	6.265360738697609
5	1.5048019443574396	4.133316015755777
6	0.8904207393933345	2.934916704510603
7	0.566674920999222	2.1791207471962233
8	0.3788125525720396	1.6648059196217213
9	0.2639946174152424	1.3052293903454657
>10	1.0906774461523296	9.866380316380392
>50	0.04878017065813035	1.8546240489901704
>100	0.03138401261114867	3.319586442436745
>500	0.0014444248411698004	0.4768905138010431
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.4336352330194868E-4
2	0.0	0.0	0.0	0.0	1.4336352330194868E-4
3	0.0	0.0	0.0	0.0	1.4336352330194868E-4
4	0.0	0.0	0.0	0.0	1.4336352330194868E-4
5	0.0	0.0	0.0	7.168176165097434E-5	1.4336352330194868E-4
6	0.0	0.0	0.0	7.168176165097434E-5	2.15045284952923E-4
7	0.0	0.0	0.0	7.168176165097434E-5	2.15045284952923E-4
8	0.0	0.0	0.0	7.168176165097434E-5	2.15045284952923E-4
9	0.0	0.0	0.0	1.4336352330194868E-4	2.15045284952923E-4
10	0.0	0.0	0.0	1.4336352330194868E-4	2.15045284952923E-4
11	0.0	0.0	0.0	1.4336352330194868E-4	2.15045284952923E-4
12	0.0	0.0	0.0	1.4336352330194868E-4	3.5840880825487167E-4
13	0.0	0.0	0.0	1.4336352330194868E-4	4.30090569905846E-4
14	0.0	0.0	0.0	1.4336352330194868E-4	4.30090569905846E-4
15	0.0	0.0	0.0	1.4336352330194868E-4	4.30090569905846E-4
16	0.0	0.0	0.0	1.4336352330194868E-4	4.30090569905846E-4
17	0.0	0.0	0.0	1.4336352330194868E-4	4.30090569905846E-4
18	0.0	0.0	0.0	1.4336352330194868E-4	5.017723315568203E-4
19	0.0	0.0	0.0	1.4336352330194868E-4	6.45135854858769E-4
20	0.0	0.0	0.0	1.4336352330194868E-4	7.168176165097433E-4
21	0.0	0.0	0.0	2.15045284952923E-4	7.168176165097433E-4
22	0.0	0.0	0.0	2.8672704660389736E-4	7.168176165097433E-4
23	0.0	0.0	0.0	5.017723315568203E-4	7.168176165097433E-4
24	0.0	0.0	0.0	5.734540932077947E-4	7.168176165097433E-4
25	0.0	0.0	0.0	5.734540932077947E-4	7.168176165097433E-4
26	0.0	0.0	0.0	6.45135854858769E-4	7.168176165097433E-4
27	0.0	0.0	0.0	0.001075226424764615	8.60181139811692E-4
28	0.0	0.0	0.0	0.002580543419435076	8.60181139811692E-4
29	0.0	0.0	0.0	0.006021267978681844	8.60181139811692E-4
30	0.0	0.0	0.0	0.011684127149108816	8.60181139811692E-4
31	0.0	0.0	0.0	0.02695234238076635	8.60181139811692E-4
32	0.0	0.0	0.0	0.043940919892047264	8.60181139811692E-4
33	0.0	0.0	0.0	0.0617179967814889	8.60181139811692E-4
34	0.0	0.0	0.0	0.08221898061366756	9.318629014626663E-4
35	0.0	0.0	0.0	0.10644741605169689	9.318629014626663E-4
36	0.0	0.0	0.0	0.14028120755095677	9.318629014626663E-4
37	0.0	0.0	0.0	0.19260889355616803	9.318629014626663E-4
38	0.0	0.0	0.0	0.2725340577970044	9.318629014626663E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	1340	0.0	29.224215	1
GGTATCA	440	0.0	26.500301	1
CGCCCTA	50	8.339651E-5	26.401247	24
TATACGG	45	0.0013991046	24.442097	5
TAATACC	110	2.0190782E-10	23.997692	4
TCAACGC	1970	0.0	19.094612	4
TATCAAC	2050	0.0	18.778683	2
CAACGCA	2020	0.0	18.730871	5
ATCAACG	2045	0.0	18.50189	3
AACGCAG	2075	0.0	18.340405	6
TAGGACG	295	0.0	17.896585	4
CGCGGTT	240	0.0	17.41749	10
TACGAAT	170	2.4683686E-9	16.823118	41
ATACGAA	170	2.4683686E-9	16.823118	40
TATACTG	250	0.0	16.718393	5
TACGACG	200	7.2759576E-11	16.498415	5
CGCAATA	175	3.621608E-9	16.3448	36
ATACCGT	270	0.0	16.294731	6
GTATAGA	230	1.8189894E-12	16.261055	1
GTGTATA	420	0.0	16.23828	1
>>END_MODULE