FastQCFastQC Report
Thu 2 Feb 2017
SRR4063930_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4063930_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences863988
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT20020.23171618124325802No Hit
TATCAACGCAGAGTACTTTTTTTTT12660.14652981291406825No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGG653.3782799E-614.6110641
ACTGTCG400.005276814.2499138
GGTATCA2400.013.8500711
TTCTAAC551.9663782E-413.8116982
GGCCGTT707.2584007E-613.570567
GTATCAA7850.013.5501341
AAGACGG500.001500638613.2976085
GTTCTAA500.001501948913.2960681
CCTAGCC500.001503916313.2937593
GTATAGC655.4621458E-513.1499581
TGAACTG957.389099E-812.9976625
GTCCTAT604.098964E-412.6629221
TTTTAGA751.48392555E-512.6607242
CCTACAC853.958303E-612.2883483
GTTTATA550.00307334512.0873361
GTTTTAG550.00307334512.0873361
ATACCTT802.864779E-511.874246
GTAAGGG802.8766892E-511.8694293
TTTAGAC658.048716E-411.6868213
GTATTAT907.474273E-611.6076781
Produced by FastQC (version 0.11.5)