##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4063930_2.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 863988 Sequences flagged as poor quality 0 Sequence length 25 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.301125710079308 32.0 32.0 32.0 32.0 32.0 2 30.998065945360352 32.0 32.0 32.0 32.0 32.0 3 30.993622596610138 32.0 32.0 32.0 32.0 32.0 4 31.012183039579252 32.0 32.0 32.0 32.0 32.0 5 30.960726306383886 32.0 32.0 32.0 32.0 32.0 6 34.60227456862827 36.0 36.0 36.0 32.0 36.0 7 34.5629441612615 36.0 36.0 36.0 32.0 36.0 8 34.54242883003005 36.0 36.0 36.0 32.0 36.0 9 34.650470839872774 36.0 36.0 36.0 32.0 36.0 10 34.428133261110105 36.0 36.0 36.0 32.0 36.0 11 34.627386028509655 36.0 36.0 36.0 32.0 36.0 12 34.488892206836205 36.0 36.0 36.0 32.0 36.0 13 34.52334407422325 36.0 36.0 36.0 32.0 36.0 14 34.44466821298444 36.0 36.0 36.0 32.0 36.0 15 34.40426371662569 36.0 36.0 36.0 32.0 36.0 16 34.39643258934152 36.0 36.0 36.0 32.0 36.0 17 34.35921795210119 36.0 36.0 36.0 32.0 36.0 18 34.34221192886938 36.0 36.0 36.0 32.0 36.0 19 34.33834497701357 36.0 36.0 36.0 32.0 36.0 20 34.334519692403134 36.0 36.0 36.0 32.0 36.0 21 34.33540280652046 36.0 36.0 36.0 32.0 36.0 22 34.30490469775043 36.0 36.0 36.0 32.0 36.0 23 34.25141205664894 36.0 36.0 36.0 32.0 36.0 24 34.23830192086001 36.0 36.0 36.0 32.0 36.0 25 33.86201544465895 36.0 36.0 36.0 32.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 4.0 5 10.0 6 52.0 7 9.0 8 47.0 9 51.0 10 74.0 11 27.0 12 64.0 13 47.0 14 71.0 15 151.0 16 242.0 17 291.0 18 397.0 19 432.0 20 716.0 21 955.0 22 1577.0 23 2345.0 24 3707.0 25 5638.0 26 8081.0 27 10903.0 28 15153.0 29 20902.0 30 27736.0 31 38113.0 32 54211.0 33 76986.0 34 172269.0 35 422726.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.919670925796204 16.930531995576835 11.76492383935343 26.38487323927354 2 16.58404180821696 19.227612868874754 37.65170236531261 26.53664295759568 3 19.302432712095502 22.629153192109946 28.717878084294203 29.350536011500346 4 13.496636402779771 15.214895202996448 34.85594173777368 36.4325266564501 5 15.062282018722225 35.70500050353458 33.752212368892906 15.480505108850288 6 34.81056690439616 34.36941103202229 16.688564327610617 14.131457735970931 7 30.483833436174805 30.378367865300437 20.222299658828717 18.915499039696037 8 27.96519995322325 33.41179630811055 19.04797742674634 19.57502631191985 9 27.37830551811348 14.908124082283026 18.29840797817397 39.41516242142953 10 16.466874122011074 26.909976965276517 31.69388022175409 24.92926869095832 11 36.456459934106526 21.562451144450296 22.30534501427306 19.675743907170112 12 24.560107197993165 23.58738037799569 28.619408905447802 23.233103518563343 13 29.622599116085574 19.985476563296242 24.780179562490158 25.61174475812803 14 23.717645805247088 19.19153312671123 25.225153229281037 31.865667838760636 15 25.259940750417503 26.71245885767361 22.1857027375716 25.841897654337288 16 25.542501343513145 25.978100735689267 23.371916868965773 25.107481051831808 17 24.111696879010104 26.097118052821028 25.03868230018391 24.752502767984954 18 24.4017791986656 25.56752499102292 25.737104863838017 24.293590946473458 19 25.43794622794053 25.178954488804052 25.483003377548798 23.90009590570662 20 25.762028260635383 24.651780739724856 25.070396758563735 24.51579424107603 21 26.11629558161111 24.541766035148356 24.843606847130253 24.498331536110285 22 26.07438107472278 24.318930258041423 25.04636220319213 24.560326464043662 23 25.176478492443916 24.53508603254108 25.239146601674083 25.04928887334092 24 24.85055082777641 24.85970136806369 25.10560765958137 25.18414014457854 25 24.92922670620396 24.537251540564334 25.316916091368206 25.216605661863507 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 45.0 1 45.0 2 110.0 3 175.0 4 175.0 5 175.0 6 428.0 7 681.0 8 681.0 9 681.0 10 896.0 11 1111.0 12 1111.0 13 1111.0 14 1617.0 15 2123.0 16 2123.0 17 2123.0 18 3787.5 19 5452.0 20 5452.0 21 5452.0 22 8192.5 23 10933.0 24 10933.0 25 10933.0 26 16210.0 27 21487.0 28 21487.0 29 21487.0 30 28149.0 31 34811.0 32 34811.0 33 34811.0 34 42550.5 35 50290.0 36 50290.0 37 50290.0 38 61542.5 39 72795.0 40 72795.0 41 72795.0 42 83256.5 43 93718.0 44 93718.0 45 93718.0 46 102164.5 47 110611.0 48 110611.0 49 110611.0 50 114825.0 51 119039.0 52 119039.0 53 119039.0 54 114374.0 55 109709.0 56 109709.0 57 109709.0 58 99984.0 59 90259.0 60 90259.0 61 90259.0 62 77379.5 63 64500.0 64 64500.0 65 64500.0 66 52076.5 67 39653.0 68 39653.0 69 39653.0 70 29747.0 71 19841.0 72 19841.0 73 19841.0 74 14701.5 75 9562.0 76 9562.0 77 9562.0 78 6910.0 79 4258.0 80 4258.0 81 4258.0 82 2922.5 83 1587.0 84 1587.0 85 1587.0 86 1096.0 87 605.0 88 605.0 89 605.0 90 410.0 91 215.0 92 215.0 93 215.0 94 172.0 95 129.0 96 129.0 97 129.0 98 264.0 99 399.0 100 399.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04085704894049454 2 0.004976920975754293 3 0.0016203928758269793 4 0.003703755144747381 5 0.010995523085968786 6 0.014004824141076035 7 0.023032727306397775 8 0.03599587031301361 9 0.050000694454089646 10 0.05891285527113802 11 0.05474613073329722 12 0.06238512571933869 13 0.06435274563998575 14 0.06666759260545285 15 0.060417505798691644 16 0.06713056199854628 17 0.06296383746070548 18 0.07847331212933513 19 0.07407510289494762 20 0.07720014629832822 21 0.07164451358120714 22 0.07835756978106177 23 0.0821770672740825 24 0.07534826872595453 25 0.07731588864660158 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 863988.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 48.26549879305223 #Duplication Level Percentage of deduplicated Percentage of total 1 63.216106579826224 30.511569158300638 2 17.409901592053203 16.80595168556804 3 7.742722346492181 11.211190682086707 4 4.074489620035556 7.866290953525199 5 2.3137739291023895 5.583772639124354 6 1.426128615580829 4.129968538443225 7 0.9624079246131826 3.2515768966689027 8 0.655195701628665 2.5298677876937075 9 0.46448514569404725 2.017674651499805 >10 1.6742381926664256 12.670109503472057 >50 0.04197600950548012 1.3991976918176519 >100 0.01785066711353905 1.5511777144071135 >500 2.412252294433514E-4 0.09116356264092079 >1k 4.824504588867028E-4 0.38048853475163363 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 2002 0.23171618124325802 No Hit TATCAACGCAGAGTACTTTTTTTTT 1266 0.14652981291406825 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.1574234827335565E-4 2 0.0 0.0 0.0 0.0 1.1574234827335565E-4 3 0.0 0.0 0.0 0.0 1.1574234827335565E-4 4 0.0 0.0 0.0 0.0 1.1574234827335565E-4 5 0.0 0.0 0.0 0.0 1.1574234827335565E-4 6 0.0 0.0 0.0 0.0 2.314846965467113E-4 7 0.0 0.0 0.0 0.0 2.314846965467113E-4 8 0.0 0.0 0.0 0.0 2.314846965467113E-4 9 0.0 0.0 0.0 0.0 2.314846965467113E-4 10 0.0 0.0 0.0 0.0 2.314846965467113E-4 11 0.0 0.0 0.0 0.0 2.314846965467113E-4 12 0.0 0.0 0.0 0.0 4.629693930934226E-4 13 0.0 0.0 0.0 0.0 5.787117413667783E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATAGG 65 3.3782799E-6 14.611064 1 ACTGTCG 40 0.0052768 14.249913 8 GGTATCA 240 0.0 13.850071 1 TTCTAAC 55 1.9663782E-4 13.811698 2 GGCCGTT 70 7.2584007E-6 13.57056 7 GTATCAA 785 0.0 13.550134 1 AAGACGG 50 0.0015006386 13.297608 5 GTTCTAA 50 0.0015019489 13.296068 1 CCTAGCC 50 0.0015039163 13.293759 3 GTATAGC 65 5.4621458E-5 13.149958 1 TGAACTG 95 7.389099E-8 12.997662 5 GTCCTAT 60 4.098964E-4 12.662922 1 TTTTAGA 75 1.48392555E-5 12.660724 2 CCTACAC 85 3.958303E-6 12.288348 3 GTTTATA 55 0.003073345 12.087336 1 GTTTTAG 55 0.003073345 12.087336 1 ATACCTT 80 2.864779E-5 11.87424 6 GTAAGGG 80 2.8766892E-5 11.869429 3 TTTAGAC 65 8.048716E-4 11.686821 3 GTATTAT 90 7.474273E-6 11.607678 1 >>END_MODULE